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ATP6V0A4 (NM_020632) Human Tagged ORF Clone
CAT＃: RC212179
ATP6V0A4 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a4 (ATP6V0A4), transcript variant 1
ORF Plasmid: tGFP
"NM_020632" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A4; ATP6N1B; ATP6N2; DRTA3; RDRTA2; RTA1C; RTADR; STV1; VPH1; VPP2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212179 representing NM_020632
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTCTGTGTTTCGAAGCGAGGAGATGTGTTTGTCACAACTGTTTCTCCAGGTGGAAGCTGCATATT GCTGTGTGGCTGAGCTCGGAGAGCTCGGATTGGTTCAGTTCAAAGATTTAAATATGAATGTGAACAGCTT TCAAAGGAAATTTGTGAATGAAGTCAGAAGGTGTGAATCACTGGAGAGAATCCTCCGTTTTCTGGAAGAC GAGATGCAAAATGAGATTGTAGTTCAGTTGCTCGAGAAAAGCCCACTGACCCCGCTCCCACGGGAAATGA TTACCCTGGAGACTGTTCTAGAAAAACTGGAAGGAGAGTTACAGGAAGCCAACCAGAACCAGCAGGCCTT GAAACAAAGCTTCCTAGAACTGACAGAACTGAAATACCTCCTGAAGAAAACCCAAGACTTCTTTGAGACG GAAACCAATTTAGCTGATGATTTCTTTACTGAGGACACTTCTGGCCTCCTGGAGTTGAAAGCAGTGCCTG CATATATGACCGGAAAGTTGGGGTTCATAGCCGGTGTGATCAACAGGGAGAGGATGGCTTCCTTTGAGCG GTTACTGTGGCGAATCTGCCGAGGAAACGTGTACTTGAAGTTCAGTGAGATGGACGCCCCTCTGGAGGAT CCTGTGACGAAAGAAGAAATTCAGAAGAACATATTCATCATATTTTACCAAGGAGAGCAGCTCAGGCAGA AAATCAAGAAGATCTGTGATGGGTTTCGAGCCACTGTCTACCCTTGCCCAGAGCCTGCGGTGGAGCGCAG AGAGATGTTGGAGAGCGTCAATGTGAGGCTGGAAGATTTAATCACCGTCATAACACAAACAGAGTCTCAC CGCCAGCGCCTGCTGCAGGAAGCCGCTGCCAACTGGCACTCCTGGCTCATCAAGGTGCAGAAGATGAAAG CTGTCTACCACATCCTGAACATGTGCAACATCGACGTCACCCAGCAGTGTGTCATCGCCGAGATCTGGTT CCCGGTGGCAGATGCCACACGTATCAAGAGGGCACTGGAGCAAGGCATGGAACTAAGTGGCTCCTCCATG GCCCCCATCATGACCACAGTGCAATCTAAAACAGCCCCTCCCACATTTAACAGGACCAATAAATTCACAG CTGGCTTCCAGAATATTGTTGATGCCTATGGTGTCGGCAGCTACCGGGAGATAAACCCAGCCCCCTACAC CATCATCACTTTCCCCTTCCTGTTCGCTGTGATGTTTGGAGACTGTGGTCATGGAACCGTGATGCTCCTG GCTGCACTTTGGATGATTCTGAATGAGAGACGCTTGCTCTCCCAGAAGACAGACAATGAGATTTGGAACA CCTTCTTCCACGGGCGCTATCTGATCCTACTTATGGGCATCTTCTCCATCTACACGGGTTTGATCTACAA TGACTGCTTCTCCAAGTCCTTGAACATCTTTGGCTCTTCTTGGAGTGTCCAACCCATGTTCAGAAACGGC ACATGGAATACTCATGTAATGGAGGAAAGTCTATATCTGCAGCTGGACCCAGCCATACCAGGAGTGTATT TTGGAAATCCATACCCGTTTGGGATTGATCCGATTTGGAACTTGGCTTCAAACAAACTCACATTTCTGAA CTCGTATAAAATGAAGATGTCGGTGATCCTGGGAATTGTCCAGATGGTTTTCGGTGTCATCCTCAGCCTT TTCAATCACATATACTTCAGAAGAACTCTCAACATCATTCTGCAATTTATCCCTGAGATGATTTTTATCC TGTGTCTGTTTGGATACCTGGTTTTCATGATCATTTTCAAATGGTGCTGCTTTGACGTCCATGTATCTCA GCACGCCCCCAGCATCCTCATCCACTTCATCAACATGTTTCTGTTTAACTACAGTGACTCTTCCAACGCA CCCCTCTACAAACATCAGCAAGAAGTCCAAAGTTTCTTTGTGGTTATGGCTTTGATTTCTGTGCCGTGGA TGCTTCTGATTAAGCCGTTTATTCTTAGAGCCAGTCATCGGAAATCCCAGCTGCAGGCATCCAGGATCCA AGAAGATGCCACTGAGAACATTGAAGGTGATAGCTCCAGCCCTTCTAGCCGTTCTGGCCAGAGGACTTCT GCAGATACCCACGGGGCTCTGGACGACCATGGAGAAGAGTTCAACTTTGGAGACGTCTTTGTCCACCAAG CCATCCACACCATCGAGTACTGCCTGGGCTGCATTTCAAACACAGCCTCCTACCTGCGGCTCTGGGCCCT CAGCCTGGCTCATGCACAACTGTCTGAAGTGCTCTGGACTATGGTGATGAACAGCGGCCTTCAGACGCGA GGCTGGGGAGGAATCGTCGGGGTTTTTATTATTTTTGCCGTATTTGCTGTCCTGACAGTAGCCATCCTTC TGATCATGGAGGGCCTCTCTGCTTTCCTGCACGCCCTGCGACTGCACTGGGTTGAGTTCCAGAACAAGTT CTATGTCGGGGATGGTTACAAGTTTTCTCCATTCTCCTTTAAACACATCCTGGATGGCACAGCCGAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212179 representing NM_020632
Red=Cloning site Green=Tags(s) MVSVFRSEEMCLSQLFLQVEAAYCCVAELGELGLVQFKDLNMNVNSFQRKFVNEVRRCESLERILRFLED EMQNEIVVQLLEKSPLTPLPREMITLETVLEKLEGELQEANQNQQALKQSFLELTELKYLLKKTQDFFET ETNLADDFFTEDTSGLLELKAVPAYMTGKLGFIAGVINRERMASFERLLWRICRGNVYLKFSEMDAPLED PVTKEEIQKNIFIIFYQGEQLRQKIKKICDGFRATVYPCPEPAVERREMLESVNVRLEDLITVITQTESH RQRLLQEAAANWHSWLIKVQKMKAVYHILNMCNIDVTQQCVIAEIWFPVADATRIKRALEQGMELSGSSM APIMTTVQSKTAPPTFNRTNKFTAGFQNIVDAYGVGSYREINPAPYTIITFPFLFAVMFGDCGHGTVMLL AALWMILNERRLLSQKTDNEIWNTFFHGRYLILLMGIFSIYTGLIYNDCFSKSLNIFGSSWSVQPMFRNG TWNTHVMEESLYLQLDPAIPGVYFGNPYPFGIDPIWNLASNKLTFLNSYKMKMSVILGIVQMVFGVILSL FNHIYFRRTLNIILQFIPEMIFILCLFGYLVFMIIFKWCCFDVHVSQHAPSILIHFINMFLFNYSDSSNA PLYKHQQEVQSFFVVMALISVPWMLLIKPFILRASHRKSQLQASRIQEDATENIEGDSSSPSSRSGQRTS ADTHGALDDHGEEFNFGDVFVHQAIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWTMVMNSGLQTR GWGGIVGVFIIFAVFAVLTVAILLIMEGLSAFLHALRLHWVEFQNKFYVGDGYKFSPFSFKHILDGTAEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020632 |
| ORF Size | 2520 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020632.3 |
| RefSeq Size | 3137 bp |
| RefSeq ORF | 2523 bp |
| Locus ID | 50617 |
| UniProt ID | Q9HBG4 |
| Protein Families | Transmembrane |
| Protein Pathways | Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection |
| MW | 96.2 kDa |
| Gene Summary | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212179L3 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal V0 subunit a4 (ATP6V0A4), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212179L4 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal V0 subunit a4 (ATP6V0A4), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG212179 | ATP6V0A4 (tGFP-tagged) - Human ATPase, H+ transporting, lysosomal V0 subunit a4 (ATP6V0A4), transcript variant 1 |
CNY 4,370.00 |
|
| SC304787 | ATP6V0A4 (untagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a4 (ATP6V0A4), transcript variant 1 |
CNY 7,220.00 |
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