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EPS15 (NM_001981) Human Tagged ORF Clone
CAT＃: RC212097
EPS15 (Myc-DDK-tagged)-Human epidermal growth factor receptor pathway substrate 15 (EPS15), transcript variant 1
ORF Plasmid: tGFP
"NM_001981" in other vectors (6)
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CNY 6,560.00
CNY 9,410.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AF-1P; AF1P; MLLT5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212097 representing NM_001981
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGGCGGCCCAGCTCTCTCTGACACAGTTATCAAGTGGGAATCCTGTATATGAAAAATACTATA GACAGGTTGATACAGGCAATACTGGAAGGGTGTTGGCTTCTGATGCTGCTGCTTTCCTGAAAAAATCAGG GCTTCCAGACTTGATACTTGGAAAGATTTGGGATTTAGCCGACACAGATGGCAAAGGTATCCTGAACAAA CAAGAATTCTTTGTTGCTTTGCGTCTTGTGGCATGTGCCCAGAATGGATTGGAAGTTTCACTAAGTAGTT TGAACCTGGCTGTTCCTCCACCAAGATTTCATGATACCAGTAGTCCTTTGCTAATCAGTGGAACCTCTGC AGCTGAGCTCCCATGGGCTGTAAAACCTGAAGATAAGGCCAAATATGATGCAATATTTGATAGTTTAAGC CCAGTGAATGGATTTCTGTCTGGTGATAAAGTGAAACCAGTGTTGCTCAACTCTAAGTTACCTGTGGATA TCCTTGGAAGAGTTTGGGAGTTGAGTGATATTGACCATGATGGAATGCTTGACAGAGATGAGTTTGCAGT TGCCATGTTTTTGGTATACTGTGCACTGGAGAAAGAACCTGTGCCAATGTCCTTGCCTCCAGCCTTGGTG CCACCATCTAAGAGAAAAACGTGGGTTGTATCCCCTGCAGAAAAAGCTAAATATGATGAAATCTTCCTGA AAACTGATAAAGATATGGACGGATTTGTGTCTGGATTGGAGGTCCGTGAAATATTCTTGAAAACAGGTTT ACCTTCTACCTTACTAGCCCATATATGGTCATTATGCGACACAAAGGACTGTGGGAAGCTTTCAAAGGAT CAGTTTGCCTTGGCTTTTCACTTAATCAGTCAGAAGTTAATCAAGGGCATTGATCCTCCTCACGTTCTTA CTCCTGAAATGATTCCACCATCAGACAGGGCCAGTTTACAAAAGAACATCATAGGATCAAGTCCTGTTGC AGATTTCTCTGCTATTAAGGAACTAGATACTCTTAACAATGAAATAGTTGACCTACAGAGGGAAAAGAAT AATGTGGAACAGGACCTTAAGGAGAAGGAAGATACTATTAAACAGAGGACAAGTGAGGTTCAGGATCTTC AAGATGAAGTTCAAAGGGAGAATACTAATCTGCAAAAACTACAGGCCCAGAAACAGCAGGTACAGGAACT CCTTGATGAACTGGATGAGCAGAAAGCCCAGCTGGAGGAGCAACTCAAGGAAGTCAGAAAGAAATGTGCT GAGGAGGCCCAACTGATCTCTTCTCTGAAAGCTGAATTAACTAGTCAGGAATCGCAGATCTCCACTTACG AAGAAGAATTGGCAAAAGCTAGAGAAGAGCTGAGCCGTCTACAGCAAGAAACAGCAGAATTGGAGGAGAG TGTAGAGTCAGGGAAGGCTCAGTTGGAACCTCTTCAGCAGCACCTACAAGATTCACAACAGGAAATTAGT TCAATGCAAATGAAACTGATGGAAATGAAAGATTTGGAAAATCATAATAGTCAGTTAAATTGGTGCAGTA GCCCACACAGCATTCTTGTAAACGGAGCTACAGATTATTGCAGCCTCAGCACCAGCAGCAGTGAAACAGC CAACCTTAATGAACATGTTGAAGGCCAGAGCAACCTAGAGTCTGAGCCCATACACCAGGAATCTCCAGCA AGAAGTAGTCCTGAACTACTGCCTTCTGGTGTGACTGATGAAAATGAGGTGACTACAGCTGTTACTGAAA AAGTTTGTTCTGAACTCGACAATAATAGACATTCAAAAGAGGAAGATCCATTTAATGTAGACTCAAGTTC GCTGACAGGTCCAGTTGCAGATACAAACTTGGATTTTTTCCAGTCTGATCCTTTTGTTGGCAGTGATCCT TTCAAGGATGATCCTTTTGGAAAAATCGATCCATTTGGTGGTGATCCTTTCAAAGGTTCAGATCCATTTG CATCAGACTGTTTCTTCAGGCAATCTACTGATCCTTTTGCCACTTCAAGCACTGACCCTTTCAGTGCAGC CAACAATAGCAGTATTACATCGGTAGAAACGTTGAAGCACAATGATCCTTTTGCTCCTGGTGGAACAGTT GTTGCAGCAAGCGATTCAGCCACAGACCCCTTTGCTTCTGTTTTTGGGAATGAATCATTTGGAGGTGGAT TTGCTGACTTCAGCACATTGTCAAAGGTCAACAATGAAGATCCTTTTCGTTCAGCCACATCGAGCTCTGT CAGCAACGTAGTGATTACAAAAAATGTATTTGAGGAAACATCGGTCAAAAGTGAAGATGAACCCCCAGCA CTGCCACCAAAGATCGGAACTCCAACAAGACCCTGCCCTCTACCACCTGGGAAAAGATCCATCAACAAAT TGGATTCTCCTGATCCCTTTAAACTGAATGATCCATTTCAGCCTTTCCCAGGCAACGATAGCCCCAAAGA AAAAGATCCTGAAATATTTTGTGATCCATTCACTTCTGCTACTACCACTACCAATAAAGAGGCTGATCCA AGCAATTTTGCCAACTTCAGTGCTTATCCCTCTGAAGAAGATATGATCGAATGGGCCAAGAGGGAAAGTG AGAGAGAGGAAGAGCAGAGGCTTGCCCGACTAAATCAGCAGGAACAAGAAGACTTAGAACTGGCTATTGC ACTCAGCAAATCTGAGATATCAGAAGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212097 representing NM_001981
Red=Cloning site Green=Tags(s) MAAAAQLSLTQLSSGNPVYEKYYRQVDTGNTGRVLASDAAAFLKKSGLPDLILGKIWDLADTDGKGILNK QEFFVALRLVACAQNGLEVSLSSLNLAVPPPRFHDTSSPLLISGTSAAELPWAVKPEDKAKYDAIFDSLS PVNGFLSGDKVKPVLLNSKLPVDILGRVWELSDIDHDGMLDRDEFAVAMFLVYCALEKEPVPMSLPPALV PPSKRKTWVVSPAEKAKYDEIFLKTDKDMDGFVSGLEVREIFLKTGLPSTLLAHIWSLCDTKDCGKLSKD QFALAFHLISQKLIKGIDPPHVLTPEMIPPSDRASLQKNIIGSSPVADFSAIKELDTLNNEIVDLQREKN NVEQDLKEKEDTIKQRTSEVQDLQDEVQRENTNLQKLQAQKQQVQELLDELDEQKAQLEEQLKEVRKKCA EEAQLISSLKAELTSQESQISTYEEELAKAREELSRLQQETAELEESVESGKAQLEPLQQHLQDSQQEIS SMQMKLMEMKDLENHNSQLNWCSSPHSILVNGATDYCSLSTSSSETANLNEHVEGQSNLESEPIHQESPA RSSPELLPSGVTDENEVTTAVTEKVCSELDNNRHSKEEDPFNVDSSSLTGPVADTNLDFFQSDPFVGSDP FKDDPFGKIDPFGGDPFKGSDPFASDCFFRQSTDPFATSSTDPFSAANNSSITSVETLKHNDPFAPGGTV VAASDSATDPFASVFGNESFGGGFADFSTLSKVNNEDPFRSATSSSVSNVVITKNVFEETSVKSEDEPPA LPPKIGTPTRPCPLPPGKRSINKLDSPDPFKLNDPFQPFPGNDSPKEKDPEIFCDPFTSATTTTNKEADP SNFANFSAYPSEEDMIEWAKRESEREEEQRLARLNQQEQEDLELAIALSKSEISEA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001981 |
| ORF Size | 2688 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001981.3 |
| RefSeq Size | 5220 bp |
| RefSeq ORF | 2691 bp |
| Locus ID | 2060 |
| UniProt ID | P42566 |
| Domains | EH, EFh |
| Protein Families | Druggable Genome |
| Protein Pathways | Endocytosis |
| MW | 98.5 kDa |
| Gene Summary | This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212097L1 | Lenti ORF clone of Human epidermal growth factor receptor pathway substrate 15 (EPS15), transcript variant 1, Myc-DDK-tagged |
CNY 8,960.00 |
|
| RC212097L2 | Lenti ORF clone of Human epidermal growth factor receptor pathway substrate 15 (EPS15), transcript variant 1, mGFP tagged |
CNY 11,310.00 |
|
| RC212097L3 | Lenti ORF clone of Human epidermal growth factor receptor pathway substrate 15 (EPS15), transcript variant 1, Myc-DDK-tagged |
CNY 11,310.00 |
|
| RC212097L4 | Lenti ORF clone of Human epidermal growth factor receptor pathway substrate 15 (EPS15), transcript variant 1, mGFP tagged |
CNY 11,310.00 |
|
| RG212097 | EPS15 (tGFP-tagged) - Human epidermal growth factor receptor pathway substrate 15 (EPS15), transcript variant 1 |
CNY 8,160.00 |
|
| SC118917 | EPS15 (untagged)-Human epidermal growth factor receptor pathway substrate 15 (EPS15), transcript variant 1 |
CNY 6,568.00 |
