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CD32B (FCGR2B) (NM_004001) Human Tagged ORF Clone
CAT＃: RC211982
FCGR2B (Myc-DDK-tagged)-Human Fc fragment of IgG, low affinity IIb, receptor (CD32) (FCGR2B), transcript variant 1
ORF Plasmid: tGFP
"NM_004001" in other vectors (6)
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CNY 3,600.00
| Cited in 3 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CD32; CD32B; FCG2; FCGR2; FCGR2C; FcRII-c; IGFR2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211982 representing NM_004001
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAATCCTGTCATTCTTACCTGTCCTTGCCACTGAGAGTGACTGGGCTGACTGCAAGTCCCCCCAGC CTTGGGGTCATATGCTTCTGTGGACAGCTGTGCTATTCCTGGCTCCTGTTGCTGGGACACCTGCAGCTCC CCCAAAGGCTGTGCTGAAACTCGAGCCCCAGTGGATCAACGTGCTCCAGGAGGACTCTGTGACTCTGACA TGCCGGGGGACTCACAGCCCTGAGAGCGACTCCATTCAGTGGTTCCACAATGGGAATCTCATTCCCACCC ACACGCAGCCCAGCTACAGGTTCAAGGCCAACAACAATGACAGCGGGGAGTACACGTGCCAGACTGGCCA GACCAGCCTCAGCGACCCTGTGCATCTGACTGTGCTTTCTGAGTGGCTGGTGCTCCAGACCCCTCACCTG GAGTTCCAGGAGGGAGAAACCATCGTGCTGAGGTGCCACAGCTGGAAGGACAAGCCTCTGGTCAAGGTCA CATTCTTCCAGAATGGAAAATCCAAGAAATTTTCCCGTTCGGATCCCAACTTCTCCATCCCACAAGCAAA CCACAGTCACAGTGGTGATTACCACTGCACAGGAAACATAGGCTACACGCTGTACTCATCCAAGCCTGTG ACCATCACTGTCCAAGCTCCCAGCTCTTCACCGATGGGGATCATTGTGGCTGTGGTCACTGGGATTGCTG TAGCGGCCATTGTTGCTGCTGTAGTGGCCTTGATCTACTGCAGGAAAAAGCGGATTTCAGCTCTCCCAGG ATACCCTGAGTGCAGGGAAATGGGAGAGACCCTCCCTGAGAAACCAGCCAATCCCACTAATCCTGATGAG GCTGACAAAGTTGGGGCTGAGAACACAATCACCTATTCACTTCTCATGCACCCGGATGCTCTGGAAGAGC CTGATGACCAGAACCGTATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211982 representing NM_004001
Red=Cloning site Green=Tags(s) MGILSFLPVLATESDWADCKSPQPWGHMLLWTAVLFLAPVAGTPAAPPKAVLKLEPQWINVLQEDSVTLT CRGTHSPESDSIQWFHNGNLIPTHTQPSYRFKANNNDSGEYTCQTGQTSLSDPVHLTVLSEWLVLQTPHL EFQEGETIVLRCHSWKDKPLVKVTFFQNGKSKKFSRSDPNFSIPQANHSHSGDYHCTGNIGYTLYSSKPV TITVQAPSSSPMGIIVAVVTGIAVAAIVAAVVALIYCRKKRISALPGYPECREMGETLPEKPANPTNPDE ADKVGAENTITYSLLMHPDALEEPDDQNRI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004001 |
| ORF Size | 930 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004001.4 |
| RefSeq Size | 1633 bp |
| RefSeq ORF | 933 bp |
| Locus ID | 2213 |
| UniProt ID | P31994 |
| Domains | ig, IGc2, IG |
| Protein Families | ES Cell Differentiation/IPS, Transmembrane |
| Protein Pathways | B cell receptor signaling pathway, Fc gamma R-mediated phagocytosis, Systemic lupus erythematosus |
| MW | 33.9 kDa |
| Gene Summary | The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
FcγRII-binding Centyrins mediate agonism and antibody-dependent cellular phagocytosis when fused to an anti-OX40 antibody
,Zhang, D;Whitaker, B;Derebe, MG;Chiu, ML;,
MAbs
,PubMed ID 29359992
[CD32B]
|
|
Fc engineering approaches to enhance the agonism and effector functions of an anti-OX40 antibody
,Zhang, D;Goldberg, MV;Chiu, ML;,
J. Biol. Chem.
,PubMed ID 27856634
[CD32B]
|
|
Ligation of Fc gamma receptor IIB inhibits antibody-dependent enhancement of dengue virus infection
,Kuan Rong Chan, Summer Li-Xin Zhang, Hwee Cheng Tan, Ying Kai Chan, Angelia Chow, Angeline Pei Chiew Lim, Subhash G. Vasudevan, Brendon J. Hanson, and Eng Eong Ooi,
PNAS, Jul 2011; 108: 12479 - 12484.
[CD32B]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211982L1 | Lenti ORF clone of Human Fc fragment of IgG, low affinity IIb, receptor (CD32) (FCGR2B), transcript variant 1, Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC211982L2 | Lenti ORF clone of Human Fc fragment of IgG, low affinity IIb, receptor (CD32) (FCGR2B), transcript variant 1, mGFP tagged |
CNY 6,000.00 |
|
| RC211982L3 | Lenti ORF clone of Human Fc fragment of IgG, low affinity IIb, receptor (CD32) (FCGR2B), transcript variant 1, Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC211982L4 | Lenti ORF clone of Human Fc fragment of IgG, low affinity IIb, receptor (CD32) (FCGR2B), transcript variant 1, mGFP tagged |
CNY 6,000.00 |
|
| RG211982 | FCGR2B (tGFP-tagged) - Human Fc fragment of IgG, low affinity IIb, receptor (CD32) (FCGR2B), transcript variant 1 |
CNY 5,200.00 |
|
| SC128159 | FCGR2B (untagged)-Human Fc fragment of IgG, low affinity IIb, receptor (CD32) (FCGR2B), transcript variant 1 |
CNY 3,600.00 |
