United States
Germany
Japan
United Kingdom
China
GTP cyclohydrolase 1 (GCH1) (NM_001024071) Human Tagged Lenti ORF Clone
CAT＃: RC211390L3
- LentiORF®
Lenti-ORF clone of GCH1 (Myc-DDK-tagged)-Human GTP cyclohydrolase 1 (GCH1), transcript variant 4
"NM_001024071" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,890.00
Product images
CNY 1,999.00
CNY 2,700.00
CNY 600.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DYT5; DYT5a; DYT14; GCH; GTP-CH-1; GTPCH1; HPABH4B |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(RC211390).
|
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NM_001024071 |
| ORF Size | 639 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001024071.1 |
| RefSeq Size | 1852 bp |
| RefSeq ORF | 642 bp |
| Locus ID | 2643 |
| UniProt ID | P30793 |
| Protein Families | Druggable Genome |
| Protein Pathways | Folate biosynthesis, Metabolic pathways |
| MW | 23.3 kDa |
| Gene Summary | This gene encodes a member of the GTP cyclohydrolase family. The encoded protein is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases. Mutations in this gene are associated with malignant hyperphenylalaninemia and dopa-responsive dystonia. Several alternatively spliced transcript variants encoding different isoforms have been described; however, not all variants give rise to a functional enzyme. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211390 | GCH1 (Myc-DDK-tagged)-Human GTP cyclohydrolase 1 (GCH1), transcript variant 4 |
CNY 3,990.00 |
|
| RC211390L4 | Lenti-ORF clone of GCH1 (mGFP-tagged)-Human GTP cyclohydrolase 1 (GCH1), transcript variant 4 |
CNY 5,890.00 |
|
| RG211390 | GCH1 (tGFP-tagged) - Human GTP cyclohydrolase 1 (GCH1), transcript variant 4 |
CNY 4,370.00 |
|
| SC302227 | GCH1 (untagged)-Human GTP cyclohydrolase 1 (GCH1), transcript variant 4 |
CNY 3,990.00 |
其它GTP cyclohydrolase 1产品
