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TRPV3 (NM_145068) Human Tagged ORF Clone
CAT＃: RC211184
TRPV3 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily V, member 3 (TRPV3)
ORF Plasmid: tGFP
"NM_145068" in other vectors (6)
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CNY 3,990.00
| Cited in 5 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FNEPPK2; OLMS; OLMS1; VRL3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211184 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGCCCACCCCAAGGAGATGGTGCCTCTCATGGGCAAGAGAGTTGCTGCCCCCAGTGGGAACCCTG CCGTCCTGCCAGAGAAGAGGCCGGCGGAGATCACCCCCACAAAGAAGAGTGCACACTTCTTCCTGGAGAT AGAAGGGTTTGAACCCAACCCCACAGTTGCCAAGACCTCTCCTCCTGTCTTCTCCAAGCCCATGGATTCC AACATCCGGCAGTGCATCTCTGGTAACTGTGATGACATGGACTCCCCCCAGTCTCCTCAAGATGATGTGA CAGAGACCCCATCCAATCCCAACAGCCCCAGTGCACAGCTGGCCAAGGAAGAGCAGAGGAGGAAAAAGGG GCGGCTGAAGAAGCGCATCTTTGCAGCCGTGTCTGAGGGCTGCGTGGAGGAGTTGGTAGAGTTGCTGGTG GAGCTGCAGGAGCTTTGCAGGCGGCGCCATGATGAGGATGTGCCTGACTTCCTCATGCACAAGCTGACGG CCTCCGACACGGGGAAGACCTGCCTGATGAAGGCCTTGTTAAACATCAACCCCAACACCAAGGAGATCGT GCGGATCCTGCTTGCCTTTGCTGAAGAGAACAACATCCTGGGCAGGTTCATCAACGCCGAGTACACAGAG GAGGCCTATGAAGGGCAGACGGCGCTGAACATCGCCATCGAGCGGCGGCAGGGGGACATCGCAGCCCTGC TCATCGCCGCCGGCGCCGACGTCAACGCGCACGCCAAGGGGGCCTTCTTCAACCCCAAGTACCAACACGA AGGCTTCTACTTCGGTGAGACGCCCCTGGCCCTGGCAGCATGCACCAACCAGCCCGAGATTGTGCAGCTG CTGATGGAGCACGAGCAGACGGACATCACCTCGCGGGACTCACGAGGCAACAACATCCTTCACGCCCTGG TGACCGTGGCCGAGGACTTCAAGACGCAGAATGACTTTGTGAAGCGCATGTACGACATGATCCTACTGCG GAGTGGCAACTGGGAGCTGGAGACCACTCGCAACAACGATGGCCTCACGCCGCTGCAGCTGGCCGCCAAG ATGGGCAAGGCGGAGATCCTGAAGTACATCCTCAGTCGTGAGATCAAGGAGAAGCGGCTCCGGAGCCTGT CCAGGAAGTTCACCGACTGGGCGTACGGACCCGTGTCATCCTCCCTCTACGACCTCACCAACGTGGACAC CACCACGGACAACTCAGTGCTGGAAATCACTGTCTACAACACCAACATCGACAACCGGCATGAGATGCTG ACCCTGGAGCCGCTGCACACGCTGCTGCATATGAAGTGGAAGAAGTTTGCCAAGCACATGTTCTTTCTGT CCTTCTGCTTTTATTTCTTCTACAACATCACCCTGACCCTCGTCTCGTACTACCGCCCCCGGGAGGAGGA GGCCATCCCGCACCCCTTGGCCCTGACGCACAAGATGGGGTGGCTGCAGCTCCTAGGGAGGATGTTTGTG CTCATCTGGGCCATGTGCATCTCTGTGAAAGAGGGCATTGCCATCTTCCTGCTGAGACCCTCGGATCTGC AGTCCATCCTCTCGGATGCCTGGTTCCACTTTGTCTTTTTTATCCAAGCTGTGCTTGTGATACTGTCTGT CTTCTTGTACTTGTTTGCCTACAAAGAGTACCTCGCCTGCCTCGTGCTGGCCATGGCCCTGGGCTGGGCG AACATGCTCTACTATACGCGGGGTTTCCAGTCCATGGGCATGTACAGCGTCATGATCCAGAAGGTCATTT TGCATGATGTTCTGAAGTTCTTGTTTGTATATATCGTGTTTTTGCTTGGATTTGGAGTAGCCTTGGCCTC GCTGATCGAGAAGTGTCCCAAAGACAACAAGGACTGCAGCTCCTACGGCAGCTTCAGCGACGCAGTGCTG GAACTCTTCAAGCTCACCATAGGCCTGGGTGATCTGAACATCCAGCAGAACTCCAAGTATCCCATTCTCT TTCTGTTCCTGCTCATCACCTATGTCATCCTCACCTTTGTTCTCCTCCTCAACATGCTCATTGCTCTGAT GGGCGAGACTGTGGAGAACGTCTCCAAGGAGAGCGAACGCATCTGGCGCCTGCAGAGAGCCAGGACCATC TTGGAGTTTGAGAAAATGTTACCAGAATGGCTGAGGAGCAGATTCCGGATGGGAGAGCTGTGCAAAGTGG CCGAGGATGATTTCCGACTGTGTTTGCGGATCAATGAGGTGAAGTGGACTGAATGGAAGACGCACGTCTC CTTCCTTAACGAAGACCCGGGGCCTGTAAGACGAACAGCAGATTTCAACAAAATCCAAGATTCTTCCAGG AACAACAGCAAAACCACTCTCAATGCATTTGAAGAAGTCGAGGAATTCCCGGAAACCTCGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211184 protein sequence
Red=Cloning site Green=Tags(s) MKAHPKEMVPLMGKRVAAPSGNPAVLPEKRPAEITPTKKSAHFFLEIEGFEPNPTVAKTSPPVFSKPMDS NIRQCISGNCDDMDSPQSPQDDVTETPSNPNSPSAQLAKEEQRRKKGRLKKRIFAAVSEGCVEELVELLV ELQELCRRRHDEDVPDFLMHKLTASDTGKTCLMKALLNINPNTKEIVRILLAFAEENNILGRFINAEYTE EAYEGQTALNIAIERRQGDIAALLIAAGADVNAHAKGAFFNPKYQHEGFYFGETPLALAACTNQPEIVQL LMEHEQTDITSRDSRGNNILHALVTVAEDFKTQNDFVKRMYDMILLRSGNWELETTRNNDGLTPLQLAAK MGKAEILKYILSREIKEKRLRSLSRKFTDWAYGPVSSSLYDLTNVDTTTDNSVLEITVYNTNIDNRHEML TLEPLHTLLHMKWKKFAKHMFFLSFCFYFFYNITLTLVSYYRPREEEAIPHPLALTHKMGWLQLLGRMFV LIWAMCISVKEGIAIFLLRPSDLQSILSDAWFHFVFFIQAVLVILSVFLYLFAYKEYLACLVLAMALGWA NMLYYTRGFQSMGMYSVMIQKVILHDVLKFLFVYIVFLLGFGVALASLIEKCPKDNKDCSSYGSFSDAVL ELFKLTIGLGDLNIQQNSKYPILFLFLLITYVILTFVLLLNMLIALMGETVENVSKESERIWRLQRARTI LEFEKMLPEWLRSRFRMGELCKVAEDDFRLCLRINEVKWTEWKTHVSFLNEDPGPVRRTADFNKIQDSSR NNSKTTLNAFEEVEEFPETSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145068 |
| ORF Size | 2373 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145068.4 |
| RefSeq Size | 6130 bp |
| RefSeq ORF | 2373 bp |
| Locus ID | 162514 |
| UniProt ID | Q8NET8 |
| Protein Families | Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane |
| MW | 90.6 kDa |
| Gene Summary | This gene product belongs to a family of nonselective cation channels that function in a variety of processes, including temperature sensation and vasoregulation. The thermosensitive members of this family are expressed in subsets of sensory neurons that terminate in the skin, and are activated at distinct physiological temperatures. This channel is activated at temperatures between 22 and 40 degrees C. This gene lies in close proximity to another family member gene on chromosome 17, and the two encoded proteins are thought to associate with each other to form heteromeric channels. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
S100A4 plays a key role in TRPV3 ion channel expression and its electrophysiological function
,Deng, S;Zhang, Y;Liao, Z;Huang, J;Huang, R;Li, Z;,
Neuroscience letters
,PubMed ID 34058292
[TRPV3]
|
|
Enhanced thermal sensitivity of TRPV3 in keratinocytes underlies heat-induced pruritogens release and pruritus in atopic dermatitis
,Seo, S;Kim, S;Kim, S;Chung, S;Lee, S;,
Journal of Investigative Dermatology
,PubMed ID 32247859
[TRPV3]
|
|
The novel high-frequency variant of TRPV3 p.A628T in East Asians showing faster sensitization in response to chemical agonists
,Choi, SW;Choi, SW;Chae, J;Yoo, HY;Kim, JI;Kim, SJ;,
Pflugers Arch.
,PubMed ID 31612282
[TRPV3]
|
|
Ablation of PM20D1 reveals N-acyl amino acid control of metabolism and nociception
,null,
Proceedings of the National Academy of Sciences of the United States of America
,PubMed ID 29967167
[TRPV3]
|
|
Identification of Significant Amino Acids in Multiple Transmembrane Domains of Human Transient Receptor Potential Ankyrin 1 (TRPA1) for Activation by Eudesmol, an Oxygenized Sesquiterpene in Hop Essential Oil
,Ohara, K;Fukuda, T;Okada, H;Kitao, S;Ishida, Y;Kato, K;Takahashi, C;Katayama, M;Uchida, K;Tominaga, M;,
J. Biol. Chem.
,PubMed ID 25525269
[TRPV3]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211184L1 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily V, member 3 (TRPV3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211184L2 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily V, member 3 (TRPV3), mGFP tagged |
CNY 5,890.00 |
|
| RC211184L3 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily V, member 3 (TRPV3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211184L4 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily V, member 3 (TRPV3), mGFP tagged |
CNY 5,890.00 |
|
| RG211184 | TRPV3 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily V, member 3 (TRPV3) |
CNY 4,370.00 |
|
| SC316997 | TRPV3 (untagged)-Human transient receptor potential cation channel, subfamily V, member 3 (TRPV3) |
CNY 5,792.00 |
