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PTGIS (NM_000961) Human Tagged ORF Clone
CAT＃: RC211030
PTGIS (Myc-DDK-tagged)-Human prostaglandin I2 (prostacyclin) synthase (PTGIS)
ORF Plasmid: tGFP
"NM_000961" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CYP8; CYP8A1; PGIS; PTGI |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211030 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTGGGCCGCGCTCCTCGGCCTCCTGGCCGCACTGTTGCTGCTGCTGCTACTGAGCCGCCGCCGCA CGCGGCGACCTGGTGAGCCTCCCCTGGACCTGGGCAGCATCCCCTGGTTGGGGTATGCCTTGGACTTTGG AAAAGATGCTGCCAGCTTCCTCACGAGGATGAAGGAGAAGCACGGTGACATCTTTACTATACTGGTTGGG GGCAGGTATGTCACCGTTCTCCTGGACCCACACTCCTACGACGCGGTGGTGTGGGAGCCTCGCACCAGGC TCGACTTCCATGCCTATGCCATCTTCCTCATGGAGAGGATTTTTGATGTGCAGCTTCCACATTACAGCCC CAGTGATGAAAAGGCCAGGATGAAACTGACTCTTCTCCACAGAGAGCTCCAGGCACTCACAGAAGCCATG TATACCAACCTCCATGCAGTGCTGTTGGGCGATGCTACAGAAGCAGGCAGTGGCTGGCACGAGATGGGTC TCCTCGACTTCTCCTACAGCTTCCTGCTCAGAGCCGGCTACCTGACTCTTTACGGAATTGAGGCGCTGCC ACGCACCCATGAAAGCCAGGCCCAGGACCGCGTCCACTCAGCTGATGTCTTCCACACCTTTCGCCAGCTC GACCGGCTGCTCCCCAAACTGGCCCGTGGCTCCCTGTCAGTGGGGGACAAGGACCACATGTGCAGTGTCA AAAGTCGCCTGTGGAAGCTGCTATCCCCAGCCAGGCTGGCCAGGCGGGCCCACCGGAGCAAATGGCTGGA GAGTTACCTGCTGCACCTGGAGGAGATGGGTGTGTCAGAGGAGATGCAGGCACGGGCCCTGGTGCTGCAG CTGTGGGCCACACAGGGGAATATGGGTCCCGCTGCCTTCTGGCTCCTGCTCTTCCTTCTCAAGAATCCTG AAGCCCTGGCTGCTGTCCGCGGAGAGCTCGAGAGTATCCTTTGGCAAGCGGAGCAGCCTGTCTCGCAGAC GACCACTCTCCCACAGAAGGTTCTAGACAGCACACCTGTGCTTGATAGCGTGCTGAGTGAGAGCCTCAGG CTTACAGCTGCCCCCTTCATCACCCGCGAGGTTGTGGTGGACCTGGCCATGCCCATGGCAGACGGGCGAG AATTCAACCTGCGACGTGGTGACCGCCTCCTCCTCTTCCCCTTCCTGAGCCCCCAGAGAGACCCAGAAAT CTACACAGACCCAGAGGTATTTAAATACAACCGATTCCTGAACCCTGACGGATCAGAGAAGAAAGACTTT TACAAGGATGGGAAACGGCTGAAGAATTACAACATGCCCTGGGGGGCGGGGCACAATCACTGCCTGGGGA GGAGTTATGCGGTCAACAGCATCAAACAATTTGTGTTCCTTGTGCTGGTGCACTTGGACTTGGAGCTGAT CAACGCAGATGTGGAGATCCCTGAGTTTGACCTCAGCAGGTACGGCTTCGGTCTGATGCAGCCGGAACAC GACGTGCCCGTCCGCTACCGCATCCGCCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211030 protein sequence
Red=Cloning site Green=Tags(s) MAWAALLGLLAALLLLLLLSRRRTRRPGEPPLDLGSIPWLGYALDFGKDAASFLTRMKEKHGDIFTILVG GRYVTVLLDPHSYDAVVWEPRTRLDFHAYAIFLMERIFDVQLPHYSPSDEKARMKLTLLHRELQALTEAM YTNLHAVLLGDATEAGSGWHEMGLLDFSYSFLLRAGYLTLYGIEALPRTHESQAQDRVHSADVFHTFRQL DRLLPKLARGSLSVGDKDHMCSVKSRLWKLLSPARLARRAHRSKWLESYLLHLEEMGVSEEMQARALVLQ LWATQGNMGPAAFWLLLFLLKNPEALAAVRGELESILWQAEQPVSQTTTLPQKVLDSTPVLDSVLSESLR LTAAPFITREVVVDLAMPMADGREFNLRRGDRLLLFPFLSPQRDPEIYTDPEVFKYNRFLNPDGSEKKDF YKDGKRLKNYNMPWGAGHNHCLGRSYAVNSIKQFVFLVLVHLDLELINADVEIPEFDLSRYGFGLMQPEH DVPVRYRIRP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000961 |
| ORF Size | 1500 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000961.4 |
| RefSeq Size | 5603 bp |
| RefSeq ORF | 1503 bp |
| Locus ID | 5740 |
| UniProt ID | Q16647 |
| Protein Families | Druggable Genome, P450, Transmembrane |
| Protein Pathways | Arachidonic acid metabolism, Metabolic pathways |
| MW | 57.1 kDa |
| Gene Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to prostacyclin (prostaglandin I2), a potent vasodilator and inhibitor of platelet aggregation. An imbalance of prostacyclin and its physiological antagonist thromboxane A2 contribute to the development of myocardial infarction, stroke, and atherosclerosis. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211030L1 | Lenti ORF clone of Human prostaglandin I2 (prostacyclin) synthase (PTGIS), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211030L2 | Lenti ORF clone of Human prostaglandin I2 (prostacyclin) synthase (PTGIS), mGFP tagged |
CNY 5,890.00 |
|
| RC211030L3 | Lenti ORF clone of Human prostaglandin I2 (prostacyclin) synthase (PTGIS), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211030L4 | Lenti ORF clone of Human prostaglandin I2 (prostacyclin) synthase (PTGIS), mGFP tagged |
CNY 5,890.00 |
|
| RG211030 | PTGIS (tGFP-tagged) - Human prostaglandin I2 (prostacyclin) synthase (PTGIS) |
CNY 4,370.00 |
|
| SC123875 | PTGIS (untagged)-Human prostaglandin I2 (prostacyclin) synthase (PTGIS) |
CNY 3,728.00 |
