United States
Germany
Japan
United Kingdom
China
PYGL (NM_002863) Human Tagged ORF Clone
CAT＃: RC210683
PYGL (Myc-DDK-tagged)-Human phosphorylase, glycogen, liver (PYGL), transcript variant 1
ORF Plasmid: tGFP
"NM_002863" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GSD6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210683 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAAGCCCCTGACGGACCAGGAGAAGCGGCGGCAGATCAGCATCCGCGGCATCGTGGGCGTGGAGA ACGTGGCAGAGCTGAAGAAGAGTTTCAACCGGCACCTGCACTTCACGCTGGTCAAGGACCGCAACGTGGC CACCACCCGCGACTACTACTTCGCGCTGGCGCACACGGTGCGCGACCACCTGGTGGGGCGCTGGATCCGC ACGCAGCAGCACTACTACGACAAGTGCCCCAAGAGGGTATATTACCTCTCTCTGGAATTTTACATGGGCC GAACATTACAGAACACCATGATCAACCTCGGTCTGCAAAATGCCTGTGATGAGGCCATTTACCAGCTTGG ATTGGATATAGAAGAGTTAGAAGAAATTGAAGAAGATGCTGGACTTGGCAATGGTGGTCTTGGGAGACTT GCTGCCTGCTTCTTGGATTCCATGGCAACCCTGGGACTTGCAGCCTATGGATACGGCATTCGGTATGAAT ATGGGATTTTCAATCAGAAGATCCGAGATGGATGGCAGGTAGAAGAAGCAGATGATTGGCTCAGATATGG AAACCCTTGGGAGAAGTCCCGCCCAGAATTCATGCTGCCTGTGCACTTCTATGGAAAAGTAGAACACACC AACACCGGGACCAAGTGGATTGACACTCAAGTGGTCCTGGCTCTGCCATATGACACCCCCGTGCCCGGCT ACATGAATAACACTGTCAACACCATGCGCCTCTGGTCTGCTCGGGCACCAAATGACTTTAACCTCAGAGA CTTTAATGTTGGAGACTACATTCAGGCTGTGCTGGACCGAAACCTGGCCGAGAACATCTCCCGGGTCCTC TATCCCAATGACAATTTTTTTGAAGGGAAGGAGCTAAGATTGAAGCAGGAATACTTTGTGGTGGCTGCAA CCTTGCAAGATATCATCCGCCGTTTCAAAGCCTCCAAGTTTGGCTCCACCCGTGGTGCAGGAACTGTGTT TGATGCCTTCCCGGATCAGGTGGCCATCCAGCTGAATGACACTCACCCTGCACTCGCGATCCCTGAGCTG ATGAGGATTTTTGTGGATATTGAAAAACTGCCCTGGTCCAAGGCATGGGAGCTCACCCAGAAGACCTTCG CCTACACCAACCACACAGTGCTCCCGGAAGCCCTGGAGCGCTGGCCCGTGGACCTGGTGGAGAAGCTGCT CCCTCGACATTTGGAAATCATTTATGAGATAAATCAGAAGCATTTAGATAGAATTGTGGCCTTGTTTCCT AAAGATGTGGACCGTCTGAGAAGGATGTCTCTGATAGAAGAGGAAGGAAGCAAAAGGATCAACATGGCCC ATCTCTGCATTGTCGGTTCCCATGCTGTGAATGGCGTGGCTAAAATCCACTCAGACATCGTGAAGACTAA AGTATTCAAGGACTTCAGTGAGCTAGAACCTGACAAGTTTCAGAATAAAACCAATGGGATCACTCCAAGG CGCTGGCTCCTACTCTGCAACCCAGGACTTGCAGAGCTCATAGCAGAGAAAATTGGAGAAGACTATGTGA AAGACCTGAGCCAGCTGACGAAGCTCCACAGCTTCCTGGGTGATGATGTCTTCCTCCGGGAACTCGCCAA GGTGAAGCAGGAGAATAAGCTGAAGTTTTCTCAGTTCCTGGAGACGGAGTACAAAGTGAAGATCAACCCA TCCTCCATGTTTGATGTCCAGGTGAAGAGGATACATGAGTACAAGCGACAGCTCTTGAACTGTCTGCATG TGATCACGATGTACAACCGCATTAAGAAAGACCCTAAGAAGTTATTCGTGCCAAGGACAGTTATCATTGG TGGTAAAGCTGCCCCAGGATATCACATGGCCAAAATGATCATAAAGCTGATCACTTCAGTGGCAGATGTG GTGAACAATGACCCTATGGTTGGAAGCAAGTTGAAAGTCATCTTCTTGGAGAACTACAGAGTATCTCTTG CTGAAAAAGTCATTCCAGCCACAGATCTGTCAGAGCAGATTTCCACTGCAGGCACCGAAGCCTCGGGGAC AGGCAATATGAAGTTCATGCTAAATGGGGCCCTAACTATCGGGACCATGGATGGGGCCAATGTGGAAATG GCAGAAGAAGCTGGGGAAGAGAACCTGTTCATCTTTGGCATGAGGATAGATGATGTGGCTGCTTTGGACA AGAAAGGGTACGAGGCAAAAGAATACTATGAGGCACTTCCAGAGCTGAAGCTGGTCATTGATCAAATTGA CAATGGCTTTTTTTCTCCCAAGCAGCCTGACCTCTTCAAAGATATCATCAACATGCTATTTTATCATGAC AGGTTTAAAGTCTTTGCAGACTACGAAGCCTATGTCAAGTGTCAAGATAAAGTGAGTCAGCTGTACATGA ATCCAAAGGCCTGGAACACAATGGTACTCAAAAACATAGCTGCCTCGGGGAAATTCTCCAGTGACCGAAC AATTAAAGAATATGCCCAAAACATCTGGAACGTGGAACCTTCAGATCTAAAGATTTCTCTATCCAATGAA TCTAACAAAGTCAATGGAAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210683 protein sequence
Red=Cloning site Green=Tags(s) MAKPLTDQEKRRQISIRGIVGVENVAELKKSFNRHLHFTLVKDRNVATTRDYYFALAHTVRDHLVGRWIR TQQHYYDKCPKRVYYLSLEFYMGRTLQNTMINLGLQNACDEAIYQLGLDIEELEEIEEDAGLGNGGLGRL AACFLDSMATLGLAAYGYGIRYEYGIFNQKIRDGWQVEEADDWLRYGNPWEKSRPEFMLPVHFYGKVEHT NTGTKWIDTQVVLALPYDTPVPGYMNNTVNTMRLWSARAPNDFNLRDFNVGDYIQAVLDRNLAENISRVL YPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKASKFGSTRGAGTVFDAFPDQVAIQLNDTHPALAIPEL MRIFVDIEKLPWSKAWELTQKTFAYTNHTVLPEALERWPVDLVEKLLPRHLEIIYEINQKHLDRIVALFP KDVDRLRRMSLIEEEGSKRINMAHLCIVGSHAVNGVAKIHSDIVKTKVFKDFSELEPDKFQNKTNGITPR RWLLLCNPGLAELIAEKIGEDYVKDLSQLTKLHSFLGDDVFLRELAKVKQENKLKFSQFLETEYKVKINP SSMFDVQVKRIHEYKRQLLNCLHVITMYNRIKKDPKKLFVPRTVIIGGKAAPGYHMAKMIIKLITSVADV VNNDPMVGSKLKVIFLENYRVSLAEKVIPATDLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEM AEEAGEENLFIFGMRIDDVAALDKKGYEAKEYYEALPELKLVIDQIDNGFFSPKQPDLFKDIINMLFYHD RFKVFADYEAYVKCQDKVSQLYMNPKAWNTMVLKNIAASGKFSSDRTIKEYAQNIWNVEPSDLKISLSNE SNKVNGN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002863 |
| ORF Size | 2541 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002863.5 |
| RefSeq Size | 2859 bp |
| RefSeq ORF | 2544 bp |
| Locus ID | 5836 |
| UniProt ID | P06737 |
| Domains | phosphorylase |
| Protein Families | Druggable Genome |
| Protein Pathways | Insulin signaling pathway, Starch and sucrose metabolism |
| MW | 97.1 kDa |
| Gene Summary | This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Feb 2011] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Inhibition of glycogen catabolism induces intrinsic apoptosis and augments multikinase inhibitors in hepatocellular carcinoma cells
,Barot, S;Abo-Ali, EM;Zhou, DL;Palaguachi, C;Dukhande, VV;,
Exp. Cell Res.
,PubMed ID 31128107
[PYGL]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210683L1 | Lenti ORF clone of Human phosphorylase, glycogen, liver (PYGL), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210683L2 | Lenti ORF clone of Human phosphorylase, glycogen, liver (PYGL), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC210683L3 | Lenti ORF clone of Human phosphorylase, glycogen, liver (PYGL), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210683L4 | Lenti ORF clone of Human phosphorylase, glycogen, liver (PYGL), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG210683 | PYGL (tGFP-tagged) - Human phosphorylase, glycogen, liver (PYGL), transcript variant 1 |
CNY 4,370.00 |
|
| SC118381 | PYGL (untagged)-Human phosphorylase, glycogen, liver (PYGL), transcript variant 1 |
CNY 6,208.00 |
