CREB1 (NM_004379) Human Tagged ORF Clone

CAT＃: RC210577

TrueORF^®

CREB1 (Myc-DDK-tagged)-Human cAMP responsive element binding protein 1 (CREB1), transcript variant A

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_004379" in other vectors (6)

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CNY 2,400.00

CNY 3,990.00

货期*

现货

规格

Cited in 7 publications.

Product images

经常一起买 (5)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

TA375073

CREB1 Rabbit polyclonal Antibody

CNY 1,999.00
CNY 3,280.00

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Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CREB; CREB-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC210577 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCATGGAATCTGGAGCCGAGAACCAGCAGAGTGGAGATGCAGCTGTAACAGAAGCTGAAAACCAAC AAATGACAGTTCAAGCCCAGCCACAGATTGCCACATTAGCCCAGGTATCTATGCCAGCAGCTCATGCAAC ATCATCTGCTCCCACCGTAACTCTAGTACAGCTGCCCAATGGGCAGACAGTTCAAGTCCATGGAGTCATT CAGGCGGCCCAGCCATCAGTTATTCAGTCTCCACAAGTCCAAACAGTTCAGATTTCAACTATTGCAGAAA GTGAAGATTCACAGGAGTCAGTGGATAGTGTAACTGATTCCCAAAAGCGAAGGGAAATTCTTTCAAGGAG GCCTTCCTACAGGAAAATTTTGAATGACTTATCTTCTGATGCACCAGGAGTGCCAAGGATTGAAGAAGAG AAGTCTGAAGAGGAGACTTCAGCACCTGCCATCACCACTGTAACGGTGCCAACTCCAATTTACCAAACTA GCAGTGGACAGTATATTGCCATTACCCAGGGAGGAGCAATACAGCTGGCTAACAATGGTACCGATGGGGT ACAGGGCCTGCAAACATTAACCATGACCAATGCAGCAGCCACTCAGCCGGGTACTACCATTCTACAGTAT GCACAGACCACTGATGGACAGCAGATCTTAGTGCCCAGCAACCAAGTTGTTGTTCAAGCTGCCTCTGGAG ACGTACAAACATACCAGATTCGCACAGCACCCACTAGCACTATTGCCCCTGGAGTTGTTATGGCATCCTC CCCAGCACTTCCTACACAGCCTGCTGAAGAAGCAGCACGAAAGAGAGAGGTCCGTCTAATGAAGAACAGG GAAGCAGCTCGAGAGTGTCGTAGAAAGAAGAAAGAATATGTGAAATGTTTAGAAAACAGAGTGGCAGTGC TTGAAAATCAAAACAAGACATTGATTGAGGAGCTAAAAGCACTTAAGGACCTTTACTGCCACAAATCAGA T ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC210577 protein sequence Red=Cloning site Green=Tags(s) MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPNGQTVQVHGVI QAAQPSVIQSPQVQTVQISTIAESEDSQESVDSVTDSQKRREILSRRPSYRKILNDLSSDAPGVPRIEEE KSEEETSAPAITTVTVPTPIYQTSSGQYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQY AQTTDGQQILVPSNQVVVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNR EAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_004379
ORF Size	981 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_004379.5
RefSeq Size	9752 bp
RefSeq ORF	984 bp
Locus ID	1385
UniProt ID	P16220
Domains	pKID, BRLZ
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Antigen processing and presentation, Huntington's disease, Melanogenesis, Prostate cancer
MW	35.1 kDa
Gene Summary	This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (7)

The use of this cDNA Clones has been cited in the following citations:
Novel live cell fluorescent probe for human-induced pluripotent stem cells highlights early reprogramming population ,Sriram, S;Kang, NY;Subramanian, S;Nandi, T;Sudhagar, S;Xing, Q;Tong, GJ;Chen, AK;Srijaya, TC;Tan, P;Loh, YH;Chang, YT;Sugii, S;, Stem cell research & therapy ,PubMed ID 33546754 [CREB1]
The endoplasmic reticulum acetyltransferases ATase1/NAT8B and ATase2/NAT8 are differentially regulated to adjust engagement of the secretory pathway ,null, Journal of neurochemistry ,PubMed ID 31945187 [CREB1]
FOXQ1 controls the induced differentiation of melanocytic cells ,Bagati, A;Bianchi-Smiraglia, A;Moparthy, S;Kolesnikova, K;Fink, EE;Kolesnikova, M;Roll, MV;Jowdy, P;Wolff, DW;Polechetti, A;Yun, DH;Lipchick, BC;Paul, LM;Wrazen, B;Moparthy, K;Mudambi, S;Morozevich, GE;Georgieva, SG;Wang, J;Shafirstein, G;Liu, S;Kandel, ES;Berman, AE;Box, NF;Paragh, G;Nikiforov, MA;, Cell Death Differ. ,PubMed ID 29463842 [CREB1]
Downregulation of microRNA-27b-3p enhances tamoxifen resistance in breast cancer by increasing NR5A2 and CREB1 expression ,Zhu, J;Zou, Z;Nie, P;Kou, X;Wu, B;Wang, S;Song, Z;He, J;, Cell Death Dis ,PubMed ID 27809310 [CREB1]
CREB Negatively Regulates IGF2R Gene Expression and Downstream Pathways to Inhibit Hypoxia-Induced H9c2 Cardiomyoblast Cell Death ,Chen, WK;Kuo, WW;Hsieh, DJ;Chang, HN;Pai, PY;Lin, KH;Pan, LF;Ho, TJ;Viswanadha, VP;Huang, CY;, Int J Mol Sci ,PubMed ID 26610485 [CREB1]
The cAMP responsive element binding protein 1 transactivates epithelial membrane protein 2, a potential tumor suppressor in the urinary bladder urothelial carcinoma ,null, Oncotarget ,PubMed ID 25940704 [CREB1]
Rit-mediated Stress Resistance Involves a p38-Mitogen- and Stress-activated Protein Kinase 1 (MSK1)-dependent cAMP Response Element-binding Protein (CREB) Activation Cascade ,Geng-Xian Shi, Weikang Cai, and Douglas A. Andres, J. Biol. Chem., Nov 2012; 287: 39859 - 39868. [CREB1]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC210577L1	Lenti ORF clone of Human cAMP responsive element binding protein 1 (CREB1), transcript variant A, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,800.00 CNY 5,890.00
RC210577L2	Lenti ORF clone of Human cAMP responsive element binding protein 1 (CREB1), transcript variant A, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,800.00 CNY 5,890.00
RC210577L3	Lenti ORF clone of Human cAMP responsive element binding protein 1 (CREB1), transcript variant A, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,800.00 CNY 5,890.00
RC210577L4	Lenti ORF clone of Human cAMP responsive element binding protein 1 (CREB1), transcript variant A, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,800.00 CNY 5,890.00
RG210577	CREB1 (tGFP-tagged) - Human cAMP responsive element binding protein 1 (CREB1), transcript variant A	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,000.00 CNY 4,370.00
SC117388	CREB1 (untagged)-Human cAMP responsive element binding protein 1 (CREB1), transcript variant A	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,400.00 CNY 6,270.00