DMT1 (SLC11A2) (NM_000617) Human Tagged ORF Clone

Name: DMT1 (SLC11A2) (NM_000617) Human Tagged ORF Clone
SKU: RC210568
Price: 4176.00

CAT＃: RC210568

SLC11A2 (Myc-DDK-tagged)-Human solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2 (SLC11A2), transcript variant 4

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_000617" in other vectors (6)

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CNY 3,990.00

货期*

现货

规格

Product images

经常一起买 (5)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

TA324527

Rabbit polyclonal SLC11A2 Antibody (Center)

CNY 6,314.00

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AHMIO1; DCT1; DMT1; NRAMP2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC210568 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCTGGGTCCTGAACAGAAGATGTCAGATGACAGTGTTTCTGGAGATCATGGGGAGTCTGCCAGTC TTGGTAACATCAACCCTGCCTATAGTAATCCCTCTCTTTCACAGTCCCCTGGGGACTCAGAGGAGTACTT CGCCACTTACTTTAATGAGAAGATCTCCATTCCTGAGGAGGAGTACTCTTGTTTTAGCTTTCGTAAACTC TGGGCTTTCACCGGACCAGGTTTTCTTATGAGCATTGCCTACCTGGATCCAGGAAATATTGAATCCGATT TGCAGTCTGGAGCAGTGGCTGGATTTAAGTTGCTCTGGATCCTTCTGTTGGCCACCCTTGTGGGGCTGCT GCTCAAGCGGCTTGCAGCTAGACTGGGAGTGGTTACTGGGCTGCATCTTGCTGAAGTATGTCACCGTCAG TATCCCAAGGTCCCACGAGTCATCCTGTGGCTGATGGTGGAGTTGGCTATCATCGGCTCAGACATGCAAG AAGTCATTGGCTCAGCCATTGCTATCAATCTTCTGTCTGTAGGAAGAATTCCTCTGTGGGGTGGCGTTCT CATCACCATTGCAGATACTTTTGTATTTCTCTTCTTGGACAAATATGGCTTGCGGAAGCTAGAAGCATTT TTTGGCTTTCTCATCACTATTATGGCCCTCACATTTGGATATGAGTATGTTACAGTGAAACCCAGCCAGA GCCAGGTACTCAAGGGCATGTTCGTACCATCCTGTTCAGGCTGTCGCACTCCACAGATTGAACAGGCTGT GGGCATCGTGGGAGCTGTCATCATGCCACACAACATGTACCTGCATTCTGCCTTAGTCAAGTCTAGACAG GTAAACCGGAACAATAAGCAGGAAGTTCGAGAAGCCAATAAGTACTTTTTCATTGAATCCTGCATTGCAC TCTTTGTTTCCTTCATCATCAATGTCTTTGTTGTCTCAGTCTTTGCTGAAGCATTTTTTGGGAAAACCAA CGAGCAGGTGGTTGAAGTCTGTACAAATACCAGCAGTCCTCATGCTGGCCTCTTTCCTAAAGATAACTCG ACACTGGCTGTGGACATCTACAAAGGGGGTGTTGTGCTGGGATGTTACTTTGGGCCTGCTGCACTCTACA TTTGGGCAGTGGGGATCCTGGCTGCAGGACAGAGCTCCACCATGACAGGAACCTATTCTGGCCAGTTTGT CATGGAGGGATTCCTGAACCTAAAGTGGTCACGCTTTGCCCGAGTGGTTCTGACTCGCTCTATTGCCATC ATCCCCACTCTGCTTGTTGCTGTCTTCCAAGATGTAGAGCATCTAACAGGGATGAATGACTTTCTGAATG TTCTACAGAGCTTACAGCTTCCCTTTGCTCTCATACCCATCCTCACATTTACGAGCTTGCGGCCAGTAAT GAGTGACTTTGCCAATGGACTAGGCTGGCGGATTGCAGGAGGAATCTTGGTCCTTATCATCTGTTCCATC AATATGTACTTTGTAGTGGTTTATGTCCGGGACCTAGGGCATGTGGCATTATATGTGGTGGCTGCTGTGG TCAGCGTGGCTTATCTGGGCTTTGTGTTCTACTTGGGTTGGCAATGTTTGATTGCACTGGGCATGTCCTT CCTGGACTGTGGGCATACGGTAAGCATCTCTAAAGGCCTGCTGACAGAAGAAGCCACCCGTGGCTACGTT AAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC210568 protein sequence Red=Cloning site Green=Tags(s) MVLGPEQKMSDDSVSGDHGESASLGNINPAYSNPSLSQSPGDSEEYFATYFNEKISIPEEEYSCFSFRKL WAFTGPGFLMSIAYLDPGNIESDLQSGAVAGFKLLWILLLATLVGLLLKRLAARLGVVTGLHLAEVCHRQ YPKVPRVILWLMVELAIIGSDMQEVIGSAIAINLLSVGRIPLWGGVLITIADTFVFLFLDKYGLRKLEAF FGFLITIMALTFGYEYVTVKPSQSQVLKGMFVPSCSGCRTPQIEQAVGIVGAVIMPHNMYLHSALVKSRQ VNRNNKQEVREANKYFFIESCIALFVSFIINVFVVSVFAEAFFGKTNEQVVEVCTNTSSPHAGLFPKDNS TLAVDIYKGGVVLGCYFGPAALYIWAVGILAAGQSSTMTGTYSGQFVMEGFLNLKWSRFARVVLTRSIAI IPTLLVAVFQDVEHLTGMNDFLNVLQSLQLPFALIPILTFTSLRPVMSDFANGLGWRIAGGILVLIICSI NMYFVVVYVRDLGHVALYVVAAVVSVAYLGFVFYLGWQCLIALGMSFLDCGHTVSISKGLLTEEATRGYV K TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_000617
ORF Size	1683 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_000617.3
RefSeq Size	4164 bp
RefSeq ORF	1686 bp
Locus ID	4891
UniProt ID	P49281
Domains	Nramp
Protein Families	Transmembrane
Protein Pathways	Lysosome
MW	61.5 kDa
Gene Summary	This gene encodes a member of the solute carrier family 11 protein family. The product of this gene transports divalent metals and is involved in iron absorption. Mutations in this gene are associated with hypochromic microcytic anemia with iron overload. A related solute carrier family 11 protein gene is located on chromosome 2. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Apr 2010]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC210568L1	Lenti ORF clone of Human solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2 (SLC11A2), transcript variant 4, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC210568L2	Lenti ORF clone of Human solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2 (SLC11A2), transcript variant 4, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC210568L3	Lenti ORF clone of Human solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2 (SLC11A2), transcript variant 4, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC210568L4	Lenti ORF clone of Human solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2 (SLC11A2), transcript variant 4, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RG210568	SLC11A2 (tGFP-tagged) - Human solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2 (SLC11A2), transcript variant 4	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,370.00
SC119791	SLC11A2 (untagged)-Human solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2 (SLC11A2), transcript variant 4	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,184.00 CNY 7,220.00

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