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PDE7B (NM_018945) Human Tagged ORF Clone
CAT＃: RC210416
PDE7B (Myc-DDK-tagged)-Human phosphodiesterase 7B (PDE7B)
ORF Plasmid: tGFP
"NM_018945" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | bA472E5.1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210416 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTGTTTAATGGTTGAGAGGTGTGGCGAAATCTTGTTTGAGAACCCCGATCAGAATGCCAAATGTG TTTGCATGCTGGGAGATATACGACTAAGGGGTCAGACGGGGGTTCGTGCTGAACGCCGTGGCTCCTACCC ATTCATTGACTTCCGCCTACTTAACAGTACAACATACTCAGGGGAGATTGGCACCAAGAAAAAGGTGAAA AGACTATTAAGCTTTCAAAGATACTTCCATGCATCAAGGCTGCTTCGTGGAATTATACCACAAGCCCCTC TGCACCTGCTGGATGAAGACTACCTTGGACAAGCAAGGCATATGCTCTCCAAAGTGGGAATGTGGGATTT TGACATTTTCTTGTTTGATCGCTTGACAAATGGAAACAGCCTGGTAACACTGTTGTGCCACCTCTTCAAT ACCCATGGACTCATTCACCATTTCAAGTTAGATATGGTGACCTTACACCGATTTTTAGTCATGGTTCAAG AAGATTACCACAGCCAAAACCCGTATCACAATGCTGTTCACGCAGCCGACGTCACCCAGGCCATGCACTG CTACCTGAAAGAGCCAAAGCTTGCCAGCTTCCTCACGCCTCTGGACATCATGCTTGGACTGCTGGCTGCA GCAGCACACGATGTGGACCACCCAGGGGTGAACCAGCCATTTTTGATAAAAACTAACCACCATCTTGCAA ACCTATATCAGAATATGTCTGTGCTGGAGAATCATCACTGGCGATCTACAATTGGCATGCTTCGAGAATC AAGGCTTCTTGCTCATTTGCCAAAGGAAATGACACAGGATATTGAACAGCAGCTGGGCTCCTTGATCTTG GCAACAGACATCAACAGGCAGAATGAATTTTTGACCAGATTGAAAGCTCACCTCCACAATAAAGACTTAA GACTGGAGGATGCACAGGACAGGCACTTTATGCTTCAGATCGCCTTGAAGTGTGCTGACATTTGCAATCC TTGTAGAATCTGGGAGATGAGCAAGCAGTGGAGTGAAAGGGTCTGTGAAGAATTCTACAGGCAAGGTGAA CTTGAACAGAAATTTGAACTGGAAATCAGTCCTCTTTGTAATCAACAGAAAGATTCCATCCCTAGTATAC AAATTGGTTTCATGAGCTACATCGTGGAGCCGCTCTTCCGGGAATGGGCCCATTTCACGGGTAACAGCAC CCTGTCGGAGAACATGCTGGGCCACCTCGCACACAACAAGGCCCAGTGGAAGAGCCTGTTGCCCAGGCAG CACAGAAGCAGGGGCAGCAGTGGCAGCGGGCCTGACCACGACCACGCAGGCCAAGGGACTGAGAGCGAGG AGCAGGAAGGCGACAGCCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210416 protein sequence
Red=Cloning site Green=Tags(s) MSCLMVERCGEILFENPDQNAKCVCMLGDIRLRGQTGVRAERRGSYPFIDFRLLNSTTYSGEIGTKKKVK RLLSFQRYFHASRLLRGIIPQAPLHLLDEDYLGQARHMLSKVGMWDFDIFLFDRLTNGNSLVTLLCHLFN THGLIHHFKLDMVTLHRFLVMVQEDYHSQNPYHNAVHAADVTQAMHCYLKEPKLASFLTPLDIMLGLLAA AAHDVDHPGVNQPFLIKTNHHLANLYQNMSVLENHHWRSTIGMLRESRLLAHLPKEMTQDIEQQLGSLIL ATDINRQNEFLTRLKAHLHNKDLRLEDAQDRHFMLQIALKCADICNPCRIWEMSKQWSERVCEEFYRQGE LEQKFELEISPLCNQQKDSIPSIQIGFMSYIVEPLFREWAHFTGNSTLSENMLGHLAHNKAQWKSLLPRQ HRSRGSSGSGPDHDHAGQGTESEEQEGDSP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018945 |
| ORF Size | 1353 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018945.4 |
| RefSeq Size | 5385 bp |
| RefSeq ORF | 1353 bp |
| Locus ID | 27115 |
| UniProt ID | Q9NP56 |
| Domains | PDEase, HDc |
| Protein Families | Druggable Genome |
| Protein Pathways | Progesterone-mediated oocyte maturation, Purine metabolism |
| MW | 51.8 kDa |
| Gene Summary | The 3',5'-cyclic nucleotides cAMP and cGMP function as second messengers in a wide variety of signal transduction pathways. 3',5'-cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of cAMP and cGMP to the corresponding 5'-monophosphates and provide a mechanism to downregulate cAMP and cGMP signaling. This gene encodes a cAMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family.[provided by RefSeq, Apr 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210416L1 | Lenti ORF clone of Human phosphodiesterase 7B (PDE7B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210416L2 | Lenti ORF clone of Human phosphodiesterase 7B (PDE7B), mGFP tagged |
CNY 5,890.00 |
|
| RC210416L3 | Lenti ORF clone of Human phosphodiesterase 7B (PDE7B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210416L4 | Lenti ORF clone of Human phosphodiesterase 7B (PDE7B), mGFP tagged |
CNY 5,890.00 |
|
| RG210416 | PDE7B (tGFP-tagged) - Human phosphodiesterase 7B (PDE7B) |
CNY 4,370.00 |
|
| SC311112 | PDE7B (untagged)-Human phosphodiesterase 7B (PDE7B) |
CNY 7,220.00 |
