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5 HT 2A (HTR2A) (NM_000621) Human Tagged ORF Clone
CAT＃: RC210182
- TrueORF®
HTR2A (Myc-DDK-tagged)-Human 5-hydroxytryptamine (serotonin) receptor 2A (HTR2A), transcript variant 1
ORF Plasmid: tGFP
"NM_000621" in other vectors (6)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5-HT2A; HTR2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210182 representing NM_000621
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATATTCTTTGTGAAGAAAATACTTCTTTGAGCTCAACTACGAACTCCCTAATGCAATTAAATGATG ACACCAGGCTCTACAGTAATGACTTTAACTCCGGAGAAGCTAACACTTCTGATGCATTTAACTGGACAGT CGACTCTGAAAATCGAACCAACCTTTCCTGTGAAGGGTGCCTCTCACCGTCGTGTCTCTCCTTACTTCAT CTCCAGGAAAAAAACTGGTCTGCTTTACTGACAGCCGTAGTGATTATTCTAACTATTGCTGGAAACATAC TCGTCATCATGGCAGTGTCCCTAGAGAAAAAGCTGCAGAATGCCACCAACTATTTCCTGATGTCACTTGC CATAGCTGATATGCTGCTGGGTTTCCTTGTCATGCCCGTGTCCATGTTAACCATCCTGTATGGGTACCGG TGGCCTCTGCCGAGCAAGCTTTGTGCAGTCTGGATTTACCTGGACGTGCTCTTCTCCACGGCCTCCATCA TGCACCTCTGCGCCATCTCGCTGGACCGCTACGTCGCCATCCAGAATCCCATCCACCACAGCCGCTTCAA CTCCAGAACTAAGGCATTTCTGAAAATCATTGCTGTTTGGACCATATCAGTAGGTATATCCATGCCAATA CCAGTCTTTGGGCTACAGGACGATTCGAAGGTCTTTAAGGAGGGGAGTTGCTTACTCGCCGATGATAACT TTGTCCTGATCGGCTCTTTTGTGTCATTTTTCATTCCCTTAACCATCATGGTGATCACCTACTTTCTAAC TATCAAGTCACTCCAGAAAGAAGCTACTTTGTGTGTAAGTGATCTTGGCACACGGGCCAAATTAGCTTCT TTCAGCTTCCTCCCTCAGAGTTCTTTGTCTTCAGAAAAGCTCTTCCAGCGGTCGATCCATAGGGAGCCAG GGTCCTACACAGGCAGGAGGACTATGCAGTCCATCAGCAATGAGCAAAAGGCATGCAAGGTGCTGGGCAT CGTCTTCTTCCTGTTTGTGGTGATGTGGTGCCCTTTCTTCATCACAAACATCATGGCCGTCATCTGCAAA GAGTCCTGCAATGAGGATGTCATTGGGGCCCTGCTCAATGTGTTTGTTTGGATCGGTTATCTCTCTTCAG CAGTCAACCCACTAGTCTACACACTGTTCAACAAGACCTATAGGTCAGCCTTTTCACGGTATATTCAGTG TCAGTACAAGGAAAACAAAAAACCATTGCAGTTAATTTTAGTGAACACAATACCGGCTTTGGCCTACAAG TCTAGCCAACTTCAAATGGGACAAAAAAAGAATTCAAAGCAAGATGCCAAGACAACAGATAATGACTGCT CAATGGTTGCTCTAGGAAAGCAGCATTCTGAAGAGGCTTCTAAAGACAATAGCGACGGAGTGAATGAAAA GGTGAGCTGTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210182 representing NM_000621
Red=Cloning site Green=Tags(s) MDILCEENTSLSSTTNSLMQLNDDTRLYSNDFNSGEANTSDAFNWTVDSENRTNLSCEGCLSPSCLSLLH LQEKNWSALLTAVVIILTIAGNILVIMAVSLEKKLQNATNYFLMSLAIADMLLGFLVMPVSMLTILYGYR WPLPSKLCAVWIYLDVLFSTASIMHLCAISLDRYVAIQNPIHHSRFNSRTKAFLKIIAVWTISVGISMPI PVFGLQDDSKVFKEGSCLLADDNFVLIGSFVSFFIPLTIMVITYFLTIKSLQKEATLCVSDLGTRAKLAS FSFLPQSSLSSEKLFQRSIHREPGSYTGRRTMQSISNEQKACKVLGIVFFLFVVMWCPFFITNIMAVICK ESCNEDVIGALLNVFVWIGYLSSAVNPLVYTLFNKTYRSAFSRYIQCQYKENKKPLQLILVNTIPALAYK SSQLQMGQKKNSKQDAKTTDNDCSMVALGKQHSEEASKDNSDGVNEKVSCV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000621 |
| ORF Size | 1413 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000621.5 |
| RefSeq Size | 3009 bp |
| RefSeq ORF | 1416 bp |
| Locus ID | 3356 |
| UniProt ID | P28223 |
| Domains | 7tm_1 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Calcium signaling pathway, Gap junction, Neuroactive ligand-receptor interaction |
| MW | 52.4 kDa |
| Gene Summary | This gene encodes one of the receptors for serotonin, a neurotransmitter with many roles. Mutations in this gene are associated with susceptibility to schizophrenia and obsessive-compulsive disorder, and are also associated with response to the antidepressant citalopram in patients with major depressive disorder (MDD). MDD patients who also have a mutation in intron 2 of this gene show a significantly reduced response to citalopram as this antidepressant downregulates expression of this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210182L1 | Lenti-ORF clone of HTR2A (Myc-DDK-tagged)-Human 5-hydroxytryptamine (serotonin) receptor 2A (HTR2A), transcript variant 1 |
CNY 7,888.00 |
|
| RC210182L2 | Lenti-ORF clone of HTR2A (mGFP-tagged)-Human 5-hydroxytryptamine (serotonin) receptor 2A (HTR2A), transcript variant 1 |
CNY 7,888.00 |
|
| RC210182L3 | Lenti-ORF clone of HTR2A (Myc-DDK-tagged)-Human 5-hydroxytryptamine (serotonin) receptor 2A (HTR2A), transcript variant 1 |
CNY 5,890.00 |
|
| RC210182L4 | Lenti-ORF clone of HTR2A (mGFP-tagged)-Human 5-hydroxytryptamine (serotonin) receptor 2A (HTR2A), transcript variant 1 |
CNY 7,888.00 |
|
| RG210182 | HTR2A (tGFP-tagged) - Human 5-hydroxytryptamine (serotonin) receptor 2A (HTR2A), transcript variant 1 |
CNY 7,088.00 |
|
| SC119793 | HTR2A (untagged)-Human 5-hydroxytryptamine (serotonin) receptor 2A (HTR2A), transcript variant 1 |
CNY 5,488.00 |
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