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EXOSC10 (NM_001001998) Human Tagged ORF Clone
CAT＃: RC210055
EXOSC10 (Myc-DDK-tagged)-Human exosome component 10 (EXOSC10), transcript variant 1
ORF Plasmid: tGFP
"NM_001001998" in other vectors (6)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | p2; p3; p4; PM-Scl; PM/Scl-100; PMSCL; PMSCL2; RRP6; Rrp6p |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210055 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCACCCAGTACCCGGGAGCCCAGGGTCCTGTCGGCGACCAGCGCAACCAAATCCGACGGAGAGA TGGTGCTGCCAGGCTTCCCGGACGCCGACAGCTTTGTGAAGTTTGCTCTTGGGTCCGTGGTGGCAGTCAC CAAGGCATCTGGGGGCCTACCACAGTTTGGCGATGAGTATGATTTTTACCGAAGTTTTCCTGGCTTCCAA GCATTTTGCGAAACACAGGGAGACAGGTTGCTTCAGTGCATGAGCAGAGTAATGCAGTACCATGGGTGTC GCAGCAACATTAAGGATCGAAGTAAAGTGACTGAGCTGGAAGACAAGTTTGATTTACTAGTTGATGCCAA TGATGTAATTCTGGAGAGAGTGGGTATTTTACTGGATGAAGCCTCAGGTGTAAACAAGAATCAACAGCCT GTCCTCCCTGCCGGCTTGCAGGTCCCCAAAACGGTAGTGTCCAGCTGGAACCGTAAGGCAGCAGAATATG GCAAAAAAGCAAAATCTGAAACTTTCCGGCTGCTTCATGCAAAAAATATCATCCGACCTCAGCTCAAGTT TCGAGAGAAGATTGACAATTCCAACACACCATTTCTTCCTAAAATCTTCATCAAACCCAATGCTCAGAAA CCTCTCCCTCAAGCTCTCTCTAAGGAAAGGCGGGAACGCCCACAGGATCGTCCTGAGGACTTGGACGTCC CCCCTGCACTGGCTGATTTCATCCATCAGCAGAGAACCCAGCAGGTTGAGCAAGACATGTTTGCACATCC TTATCAATATGAACTAAATCACTTTACCCCAGCAGATGCAGTGCTTCAAAAGCCACAACCCCAGTTATAC AGACCTATAGAAGAGACACCATGCCATTTCATATCCTCCCTGGATGAACTCGTGGAACTCAACGAAAAGC TCTTGAATTGTCAGGAATTTGCAGTTGACTTGGAGCACCACTCTTACAGGAGCTTCCTGGGACTGACCTG CCTGATGCAAATTTCTACTCGGACGGAAGACTTCATCATTGACACCCTCGAGCTTCGAAGTGACATGTAC ATTCTCAATGAGAGCCTCACAGACCCAGCCATCGTTAAGGTCTTTCATGGTGCTGATTCAGACATAGAAT GGCTACAGAAAGACTTTGGGTTGTATGTAGTAAACATGTTTGATACTCATCAGGCAGCACGCCTTCTTAA CCTGGGCAGGCACTCACTCGATCATCTCCTGAAACTCTACTGCAACGTGGACTCAAACAAGCAATATCAG CTGGCTGATTGGAGAATACGCCCTCTGCCCGAGGAGATGCTCAGCTACGCCCGGGATGACACCCATTACC TGCTATATATCTATGACAAAATGAGGCTGGAGATGTGGGAGCGCGGCAACGGGCAGCCCGTGCAGCTGCA GGTGGTGTGGCAACGGAGCAGGGACATCTGCCTCAAGAAATTCATCAAACCTATCTTCACGGATGAGTCC TACCTTGAACTCTATAGGAAGCAGAAGAAGCACCTTAACACACAGCAGTTGACAGCCTTTCAGCTGCTGT TTGCCTGGAGGGATAAAACAGCTCGCAGGGAAGATGAAAGTTACGGATATGTACTGCCAAACCACATGAT GCTGAAAATAGCTGAAGAACTGCCTAAGGAACCTCAGGGCATCATAGCTTGCTGCAACCCAGTACCGCCC CTTGTGCGGCAGCAGATCAACGAAATGCACCTTTTAATCCAGCAGGCCCGAGAGATGCCCCTGCTCAAGT CTGAAGTTGCAGCCGGAGTGAAGAAGAGCGGACCGCTGCCCAGTGCTGAGAGATTGGAGAATGTTCTCTT TGGACCTCACGACTGCTCCCATGCCCCTCCGGATGGCTATCCAATCATCCCAACCAGTGGATCTGTGCCA GTTCAGAAGCAGGCGAGCCTCTTCCCTGATGAAAAAGAAGATAACTTGCTGGGTACCACATGCCTGATTG CCACAGCTGTCATCACGTTATTTAATGAACCTAGTGCTGAAGACAGTAAAAAGGGTCCATTGACAGTTGC ACAGAAAAAAGCCCAGAACATCATGGAGTCCTTTGAAAATCCATTTAGGATGTTTCTGCCCTCACTGGGA CACCGTGCTCCCGTCTCTCAGGCAGCGAAGTTCGATCCATCAACCAAAATCTATGAAATCAGCAACCGTT GGAAGCTGGCCCAGGTACAAGTACAAAAAGACTCTAAAGAAGCTGTCAAGAAGAAGGCAGCTGAGCAAAC AGCTGCCCGGGAACAGGCAAAGGAGGCGTGCAAAGCTGCAGCAGAACAGGCCATCTCCGTCCGACAGCAG GTCGTGCTAGAAAATGCTGCAAAGAAGAGAGAGCGAGCAACAAGCGACCCAAGGACCACAGAACAGAAAC AAGAGAAGAAACGACTCAAAATTTCCAAGAAGCCAAAGGACCCAGAGCCACCAGAAAAAGAGTTTACGCC TTACGACTACAGCCAGTCAGACTTCAAGGCTTTTGCTGGAAACAGCAAATCCAAAGTTTCTTCTCAGTTT GATCCAAATAAACAGACCCCGTCTGGCAAGAAATGCATTGCAGCCAAAAAAATTAAACAGTCGGTGGGAA ACAAAAGCATGTCCTTTCCAACTGGAAAGTCAGACAGAGGCTTCAGGTACAACTGGCCACAGAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210055 protein sequence
Red=Cloning site Green=Tags(s) MAPPSTREPRVLSATSATKSDGEMVLPGFPDADSFVKFALGSVVAVTKASGGLPQFGDEYDFYRSFPGFQ AFCETQGDRLLQCMSRVMQYHGCRSNIKDRSKVTELEDKFDLLVDANDVILERVGILLDEASGVNKNQQP VLPAGLQVPKTVVSSWNRKAAEYGKKAKSETFRLLHAKNIIRPQLKFREKIDNSNTPFLPKIFIKPNAQK PLPQALSKERRERPQDRPEDLDVPPALADFIHQQRTQQVEQDMFAHPYQYELNHFTPADAVLQKPQPQLY RPIEETPCHFISSLDELVELNEKLLNCQEFAVDLEHHSYRSFLGLTCLMQISTRTEDFIIDTLELRSDMY ILNESLTDPAIVKVFHGADSDIEWLQKDFGLYVVNMFDTHQAARLLNLGRHSLDHLLKLYCNVDSNKQYQ LADWRIRPLPEEMLSYARDDTHYLLYIYDKMRLEMWERGNGQPVQLQVVWQRSRDICLKKFIKPIFTDES YLELYRKQKKHLNTQQLTAFQLLFAWRDKTARREDESYGYVLPNHMMLKIAEELPKEPQGIIACCNPVPP LVRQQINEMHLLIQQAREMPLLKSEVAAGVKKSGPLPSAERLENVLFGPHDCSHAPPDGYPIIPTSGSVP VQKQASLFPDEKEDNLLGTTCLIATAVITLFNEPSAEDSKKGPLTVAQKKAQNIMESFENPFRMFLPSLG HRAPVSQAAKFDPSTKIYEISNRWKLAQVQVQKDSKEAVKKKAAEQTAAREQAKEACKAAAEQAISVRQQ VVLENAAKKRERATSDPRTTEQKQEKKRLKISKKPKDPEPPEKEFTPYDYSQSDFKAFAGNSKSKVSSQF DPNKQTPSGKKCIAAKKIKQSVGNKSMSFPTGKSDRGFRYNWPQR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001001998 |
| ORF Size | 2655 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001001998.3 |
| RefSeq Size | 2834 bp |
| RefSeq ORF | 2658 bp |
| Locus ID | 5394 |
| UniProt ID | Q01780 |
| Protein Pathways | RNA degradation |
| MW | 100.8 kDa |
| Gene Summary | Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 has 3'-5' exonuclease activity (By similarity). EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of MTREX, C1D and MPP6 wth the RNA exosome involved in the maturation of 5.8S rRNA.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210055L1 | Lenti ORF clone of Human exosome component 10 (EXOSC10), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210055L2 | Lenti ORF clone of Human exosome component 10 (EXOSC10), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC210055L3 | Lenti ORF clone of Human exosome component 10 (EXOSC10), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210055L4 | Lenti ORF clone of Human exosome component 10 (EXOSC10), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG210055 | EXOSC10 (tGFP-tagged) - Human exosome component 10 (EXOSC10), transcript variant 1 |
CNY 4,370.00 |
|
| SC124367 | EXOSC10 (untagged)-Human exosome component 10 (EXOSC10), transcript variant 1 |
CNY 2,950.00 |
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