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ATP6V0A2 (NM_012463) Human Tagged ORF Clone
CAT＃: RC209819
- TrueORF®
ATP6V0A2 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a2 (ATP6V0A2)
ORF Plasmid: tGFP
"NM_012463" in other vectors (6)
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CNY 9,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D; J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209819 representing NM_012463
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTCCCTGTTCCGGAGCGAGACCATGTGCCTGGCGCAGCTCTTCCTGCAGTCGGGCACGGCCTACG AGTGCCTCAGCGCCCTGGGCGAGAAAGGCCTGGTCCAGTTCCGAGACCTCAACCAGAACGTAAGTTCTTT CCAAAGAAAATTTGTTGGTGAGGTGAAGAGGTGTGAAGAGCTAGAGCGAATATTGGTGTATTTGGTACAG GAAATTAATAGAGCTGATATTCCCCTTCCTGAAGGAGAGGCCAGCCCTCCTGCGCCACCCCTGAAACAGG TTCTAGAAATGCAGGAGCAGTTGCAGAAGCTCGAGGTTGAACTGAGAGAAGTCACTAAGAACAAGGAGAA ACTGAGGAAAAACTTGCTGGAACTGATAGAGTACACTCACATGCTGAGAGTGACAAAGACCTTTGTGAAA CGCAATGTTGAGTTTGAACCCACTTATGAAGAATTCCCTTCCTTAGAGAGTGATTCTTTGTTGGATTACA GCTGTATGCAGAGGCTGGGAGCAAAACTGGGATTTGTGTCTGGCCTAATTAACCAAGGAAAAGTGGAAGC ATTTGAAAAAATGTTGTGGAGAGTCTGCAAAGGGTACACCATCGTGTCCTATGCAGAACTGGATGAATCC CTTGAAGACCCTGAAACAGGGGAAGTCATAAAATGGTATGTCTTTTTAATATCCTTTTGGGGAGAGCAGA TTGGCCACAAGGTTAAGAAGATATGTGATTGCTACCACTGCCACGTGTACCCCTATCCAAACACAGCCGA GGAGCGGAGGGAGATCCAGGAGGGGCTGAACACCCGCATCCAGGATCTCTACACTGTACTGCACAAAACC GAGGACTATTTGAGGCAAGTGCTATGTAAAGCCGCCGAGTCTGTCTACAGCCGTGTGATCCAGGTGAAGA AAATGAAGGCCATCTATCACATGCTGAACATGTGCAGCTTTGACGTGACCAACAAGTGCCTCATTGCTGA GGTCTGGTGTCCCGAGGCGGATCTGCAGGACCTGCGCCGGGCACTGGAGGAGGGCTCGAGAGAGAGTGGT GCTACAATCCCCTCATTCATGAATATAATCCCCACAAAAGAAACACCCCCCACTCGGATCCGCACCAACA AATTCACCGAGGGATTTCAGAACATCGTGGATGCTTATGGAGTCGGAAGCTACAGAGAAGTCAATCCAGC TCTCTTTACCATCATCACCTTCCCGTTTTTATTTGCTGTGATGTTTGGAGACTTCGGACATGGCTTTGTG ATGTTTTTATTTGCCCTCTTGTTGGTGTTAAATGAAAATCATCCCAGACTAAATCAGTCACAAGAGATCA TGAGGATGTTTTTTAATGGCCGGTACATCCTCCTGCTGATGGGGCTGTTCTCAGTGTACACTGGCCTCAT CTACAACGACTGCTTTTCAAAGTCAGTCAACCTGTTCGGCTCTGGGTGGAACGTGTCGGCCATGTACAGC TCCAGCCACCCACCCGCAGAGCATAAGAAGATGGTGCTTTGGAATGACAGCGTCGTTAGACACAACAGCA TTTTGCAGCTGGATCCAAGCATTCCTGGAGTGTTCCGAGGCCCTTATCCCCTTGGCATTGATCCTATTTG GAACTTGGCCACAAATCGCCTCACTTTTCTAAACTCTTTCAAAATGAAAATGTCCGTGATTTTAGGAATC ATTCATATGACTTTTGGAGTCATTCTGGGAATATTTAACCACTTGCACTTCAGGAAGAAGTTCAACATTT ACCTGGTTTCCATCCCGGAACTTCTCTTCATGCTCTGTATCTTTGGATACCTTATATTTATGATTTTCTA CAAGTGGCTGGTTTTTTCAGCAGAAACCTCCAGAGTTGCTCCCAGCATTCTGATTGAATTTATTAACATG TTTTTATTCCCAGCCAGTAAAACAAGTGGCCTTTACACAGGGCAGGAGTATGTCCAGAGAGTGCTGCTGG TTGTCACAGCATTGTCTGTCCCTGTCCTCTTCTTGGGAAAGCCACTGTTTTTGTTGTGGCTTCACAATGG GCGTAGTTGCTTCGGGGTGAACCGGAGTGGCTACACACTTATAAGGAAAGATAGTGAGGAAGAAGTTTCA TTGCTGGGAAGCCAAGATATAGAAGAGGGAAATCACCAGGTGGAAGATGGATGTAGAGAAATGGCGTGTG AAGAGTTTAATTTTGGAGAAATATTAATGACCCAAGTAATCCATTCCATCGAGTACTGTCTGGGATGCAT CTCCAACACCGCCTCCTACCTGAGGCTCTGGGCGCTTAGCCTGGCTCACGCACAGTTGTCTGATGTCCTG TGGGCCATGCTGATGCGCGTGGGCCTCCGCGTTGACACCACCTATGGCGTCTTGCTACTGCTCCCGGTTA TCGCGCTCTTTGCAGTTTTGACCATTTTCATCCTTCTGATCATGGAAGGGCTTTCTGCGTTTCTTCACGC CATACGCCTCCACTGGGTAGAATTTCAGAACAAATTCTACGTTGGTGCAGGCACCAAATTTGTTCCTTTC TCATTCAGTCTACTTTCATCAAAGTTCAATAACGACGACAGTGTGGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209819 representing NM_012463
Red=Cloning site Green=Tags(s) MGSLFRSETMCLAQLFLQSGTAYECLSALGEKGLVQFRDLNQNVSSFQRKFVGEVKRCEELERILVYLVQ EINRADIPLPEGEASPPAPPLKQVLEMQEQLQKLEVELREVTKNKEKLRKNLLELIEYTHMLRVTKTFVK RNVEFEPTYEEFPSLESDSLLDYSCMQRLGAKLGFVSGLINQGKVEAFEKMLWRVCKGYTIVSYAELDES LEDPETGEVIKWYVFLISFWGEQIGHKVKKICDCYHCHVYPYPNTAEERREIQEGLNTRIQDLYTVLHKT EDYLRQVLCKAAESVYSRVIQVKKMKAIYHMLNMCSFDVTNKCLIAEVWCPEADLQDLRRALEEGSRESG ATIPSFMNIIPTKETPPTRIRTNKFTEGFQNIVDAYGVGSYREVNPALFTIITFPFLFAVMFGDFGHGFV MFLFALLLVLNENHPRLNQSQEIMRMFFNGRYILLLMGLFSVYTGLIYNDCFSKSVNLFGSGWNVSAMYS SSHPPAEHKKMVLWNDSVVRHNSILQLDPSIPGVFRGPYPLGIDPIWNLATNRLTFLNSFKMKMSVILGI IHMTFGVILGIFNHLHFRKKFNIYLVSIPELLFMLCIFGYLIFMIFYKWLVFSAETSRVAPSILIEFINM FLFPASKTSGLYTGQEYVQRVLLVVTALSVPVLFLGKPLFLLWLHNGRSCFGVNRSGYTLIRKDSEEEVS LLGSQDIEEGNHQVEDGCREMACEEFNFGEILMTQVIHSIEYCLGCISNTASYLRLWALSLAHAQLSDVL WAMLMRVGLRVDTTYGVLLLLPVIALFAVLTIFILLIMEGLSAFLHAIRLHWVEFQNKFYVGAGTKFVPF SFSLLSSKFNNDDSVA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012463 |
| ORF Size | 2568 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012463.4 |
| RefSeq Size | 4681 bp |
| RefSeq ORF | 2571 bp |
| Locus ID | 23545 |
| UniProt ID | Q9Y487 |
| Domains | V_ATPase_sub_a |
| Protein Families | Transmembrane |
| Protein Pathways | Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection |
| MW | 97.9 kDa |
| Gene Summary | The protein encoded by this gene is a subunit of the vacuolar ATPase (v-ATPase), an heteromultimeric enzyme that is present in intracellular vesicles and in the plasma membrane of specialized cells, and which is essential for the acidification of diverse cellular components. V-ATPase is comprised of a membrane peripheral V(1) domain for ATP hydrolysis, and an integral membrane V(0) domain for proton translocation. The subunit encoded by this gene is a component of the V(0) domain. Mutations in this gene are a cause of both cutis laxa type II and wrinkly skin syndrome. [provided by RefSeq, Jul 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209819L1 | Lenti-ORF clone of ATP6V0A2 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a2 (ATP6V0A2) |
CNY 11,800.00 |
|
| RC209819L2 | Lenti-ORF clone of ATP6V0A2 (mGFP-tagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a2 (ATP6V0A2) |
CNY 9,030.00 |
|
| RC209819L3 | Lenti-ORF clone of ATP6V0A2 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a2 (ATP6V0A2) |
CNY 9,030.00 |
|
| RC209819L4 | Lenti-ORF clone of ATP6V0A2 (mGFP-tagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a2 (ATP6V0A2) |
CNY 9,030.00 |
|
| RG209819 | ATP6V0A2 (tGFP-tagged) - Human ATPase, H+ transporting, lysosomal V0 subunit a2 (ATP6V0A2) |
CNY 7,890.00 |
|
| SC115366 | ATP6V0A2 (untagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a2 (ATP6V0A2) |
CNY 9,416.00 |
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