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ATG4D (NM_032885) Human Tagged ORF Clone
CAT＃: RC209627
- TrueORF®
ATG4D (Myc-DDK-tagged)-Human ATG4 autophagy related 4 homolog D (S. cerevisiae) (ATG4D)
ORF Plasmid: tGFP
"NM_032885" in other vectors (5)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | APG4-D; APG4D; AUTL4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209627 representing NM_032885
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTCAGTGTCGCCGGCCGCCGCGCAGTACCGGAGCAGCAGCCCGGAGGACGCGCGCCGCCGGCCCG AGGCCCGCAGGCCGCGGGGTCCCAGAGGCCCAGACCCCAACGGCCTGGGGCCTTCCGGAGCCAGCGGCCC CGCTCTTGGCTCTCCCGGGGCTGGCCCGAGTGAGCCGGACGAAGTGGACAAGTTCAAGGCCAAGTTCCTG ACAGCCTGGAACAACGTCAAGTACGGTTGGGTGGTTAAAAGCCGGACCAGCTTTAGCAAGATCTCCAGCA TCCACCTCTGTGGCCGCCGCTACCGTTTCGAGGGCGAGGGTGACATACAGCGTTTCCAGCGGGACTTTGT GTCCCGCCTGTGGCTCACATACCGCCGGGACTTCCCGCCCCTTCCTGGGGGCTGCCTGACCTCGGACTGT GGCTGGGGGTGCATGTTACGCAGCGGCCAGATGATGCTGGCACAGGGCCTTCTGCTGCATTTCCTGCCCA GAGACTGGACATGGGCCGAGGGCATGGGCCTGGGCCCCCCTGAGCTGTCAGGGTCAGCCTCTCCCAGCCG GTACCATGGGCCTGCCCGCTGGATGCCCCCACGCTGGGCCCAGGGTGCCCCTGAGCTGGAGCAGGAACGC CGGCACCGGCAGATTGTGTCCTGGTTCGCCGACCACCCCCGGGCCCCCTTTGGCCTACACCGGCTGGTGG AGCTTGGGCAGAGCTCAGGCAAGAAGGCAGGTGACTGGTATGGGCCATCGCTAGTGGCACACATCCTCAG GAAAGCCGTGGAGAGCTGCTCCGACGTCACCCGCCTGGTGGTGTACGTTTCTCAGGACTGCACAGTGTAC AAGGCGGATGTGGCACGCCTGGTGGCCAGGCCAGACCCCACAGCCGAGTGGAAGTCTGTGGTCATCCTGG TGCCCGTGCGACTGGGTGGCGAGACTCTCAACCCCGTGTATGTGCCCTGCGTGAAGGAACTCCTGCGTTG CGAGCTGTGCCTGGGCATCATGGGTGGGAAACCGCGACACTCACTGTACTTCATTGGCTACCAAGATGAC TTCCTGCTGTACCTGGACCCTCACTACTGCCAGCCCACTGTGGATGTCAGCCAGGCCGACTTCCCCCTGG AGTCCTTCCACTGCACCTCGCCCCGCAAGATGGCCTTTGCCAAGATGGACCCAAGCTGTACCGTGGGCTT CTATGCTGGAGACAGGAAGGAGTTTGAGACACTCTGCTCAGAGCTGACCAGGGTCCTCAGCTCCTCCTCA GCCACAGAGCGGTACCCCATGTTCACCCTGGCCGAGGGCCATGCTCAGGACCACAGCCTGGACGACCTCT GCTCCCAGCTCGCCCAGCCCACACTCCGGCTCCCTCGCACAGGGCGGCTCCTCAGGGCCAAACGCCCCAG CTCTGAGGACTTTGTGTTTTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209627 representing NM_032885
Red=Cloning site Green=Tags(s) MNSVSPAAAQYRSSSPEDARRRPEARRPRGPRGPDPNGLGPSGASGPALGSPGAGPSEPDEVDKFKAKFL TAWNNVKYGWVVKSRTSFSKISSIHLCGRRYRFEGEGDIQRFQRDFVSRLWLTYRRDFPPLPGGCLTSDC GWGCMLRSGQMMLAQGLLLHFLPRDWTWAEGMGLGPPELSGSASPSRYHGPARWMPPRWAQGAPELEQER RHRQIVSWFADHPRAPFGLHRLVELGQSSGKKAGDWYGPSLVAHILRKAVESCSDVTRLVVYVSQDCTVY KADVARLVARPDPTAEWKSVVILVPVRLGGETLNPVYVPCVKELLRCELCLGIMGGKPRHSLYFIGYQDD FLLYLDPHYCQPTVDVSQADFPLESFHCTSPRKMAFAKMDPSCTVGFYAGDRKEFETLCSELTRVLSSSS ATERYPMFTLAEGHAQDHSLDDLCSQLAQPTLRLPRTGRLLRAKRPSSEDFVFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032885 |
| ORF Size | 1422 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032885.6 |
| RefSeq Size | 1929 bp |
| RefSeq ORF | 1425 bp |
| Locus ID | 84971 |
| UniProt ID | Q86TL0 |
| Domains | Peptidase_C54 |
| Protein Pathways | Regulation of autophagy |
| MW | 52.7 kDa |
| Gene Summary | Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209627L3 | Lenti-ORF clone of ATG4D (Myc-DDK-tagged)-Human ATG4 autophagy related 4 homolog D (S. cerevisiae) (ATG4D) |
CNY 5,890.00 |
|
| RC209627L4 | Lenti-ORF clone of ATG4D (mGFP-tagged)-Human ATG4 autophagy related 4 homolog D (S. cerevisiae) (ATG4D) |
CNY 5,890.00 |
|
| RG209627 | ATG4D (tGFP-tagged) - Human ATG4 autophagy related 4 homolog D (S. cerevisiae) (ATG4D) |
CNY 4,370.00 |
|
| SC102914 | ATG4D (untagged)-Human ATG4 autophagy related 4 homolog D (S. cerevisiae) (ATG4D) |
CNY 2,400.00 |
|
| SC317783 | ATG4D (untagged)-Human ATG4 autophagy related 4 homolog D (S. cerevisiae) (ATG4D) |
CNY 7,220.00 |
