United States
Germany
Japan
United Kingdom
China
Hexokinase II (HK2) (NM_000189) Human Tagged ORF Clone
CAT＃: RC209482
HK2 (Myc-DDK-tagged)-Human hexokinase 2 (HK2)
ORF Plasmid: tGFP
"NM_000189" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 10,072.00
| Cited in 4 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HKII; HXK2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209482 representing NM_000189
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTGCCTCGCATCTGCTTGCCTACTTCTTCACGGAGCTCAACCATGACCAAGTGCAGAAGGTTGACC AGTATCTCTACCACATGCGCCTCTCTGATGAGACCCTCTTGGAGATCTCTAAGCGGTTCCGCAAGGAGAT GGAGAAAGGGCTTGGAGCCACCACTCACCCTACTGCAGCAGTGAAGATGCTGCCCACCTTTGTGAGGTCC ACTCCAGATGGGACAGAACACGGAGAGTTCCTGGCTCTGGATCTTGGAGGGACCAACTTCCGTGTGCTTT GGGTGAAAGTAACGGACAATGGGCTCCAGAAGGTGGAGATGGAGAATCAGATCTATGCCATCCCTGAGGA CATCATGCGAGGCAGTGGCACCCAGCTGTTTGACCACATTGCCGAATGCCTGGCTAACTTCATGGATAAG CTACAAATCAAAGACAAGAAGCTCCCACTGGGTTTTACCTTCTCGTTCCCCTGCCACCAGACTAAACTAG ACGAGAGTTTCCTGGTCTCATGGACCAAGGGATTCAAGTCCAGTGGAGTGGAAGGCAGAGACGTTGTGGC TCTGATCCGGAAGGCCATCCAGAGGAGAGGGGACTTTGATATCGACATTGTGGCTGTGGTGAATGACACA GTTGGGACCATGATGACCTGTGGTTATGATGACCACAACTGTGAGATTGGTCTCATTGTGGGCACGGGCA GCAACGCCTGCTACATGGAAGAGATGCGCCACATCGACATGGTGGAAGGCGATGAGGGGCGGATGTGTAT CAATATGGAGTGGGGGGCCTTCGGGGACGATGGCTCGCTCAACGACATTCGCACTGAGTTTGACCAGGAG ATTGACATGGGCTCACTGAACCCGGGAAAGCAACTGTTTGAGAAGATGATCAGTGGGATGTACATGGGGG AGCTGGTGAGGCTTATCCTGGTGAAGATGGCCAAGGAGGAGCTGCTCTTTGGGGGGAAGCTCAGCCCAGA GCTTCTCAACACCGGTCGCTTTGAGACCAAAGACATCTCAGACATTGAAGGGGAGAAGGATGGCATCCGG AAGGCCCGTGAGGTCCTGATGCGGTTGGGCCTGGACCCGACTCAGGAGGACTGCGTGGCCACTCACCGGA TCTGCCAGATCGTGTCCACACGCTCCGCCAGCCTGTGCGCAGCCACCCTGGCCGCCGTGCTGCAGCGCAT CAAGGAGAACAAAGGCGAGGAGCGGCTGCGCTCTACTATTGGGGTCGACGGTTCCGTCTACAAGAAACAC CCCCATTTTGCCAAGCGTCTACATAAGACCGTGCGGCGGCTGGTGCCCGGCTGCGATGTCCGCTTCCTCC GCTCCGAGGATGGCAGTGGCAAAGGTGCAGCCATGGTGACAGCAGTGGCTTACCGGCTGGCCGATCAACA CCGTGCCCGCCAGAAGACATTAGAGCATCTGCAGCTGAGCCATGACCAGCTGCTGGAGGTCAAGAGGAGG ATGAAGGTAGAAATGGAGCGAGGTCTGAGCAAGGAGACTCATGCCAGTGCCCCCGTCAAGATGCTGCCCA CCTACGTGTGTGCTACCCCGGACGGCACAGAGAAAGGGGACTTCTTGGCCTTGGACCTTGGAGGAACAAA TTTCCGGGTCCTGCTGGTCCGTGTTCGGAATGGGAAGTGGGGTGGAGTGGAGATGCACAACAAGATCTAC GCCATCCCGCAGGAGGTCATGCACGGCACCGGGGACGAGCTCTTTGACCACATTGTCCAGTGCATCGCGG ACTTCCTCGAGTACATGGGCATGAAGGGCGTGTCCCTGCCTCTGGGTTTTACCTTCTCCTTCCCCTGCCA GCAGAACAGCCTGGACGAGAGCATCCTCCTCAAGTGGACAAAAGGCTTCAAGGCATCTGGCTGCGAGGGC GAGGACGTGGTGACCCTGCTGAAGGAAGCGATCCACCGGCGAGAGGAGTTTGACCTGGATGTGGTTGCTG TGGTGAACGACACAGTCGGAACTATGATGACCTGTGGCTTTGAAGACCCTCACTGTGAAGTTGGCCTCAT TGTTGGCACGGGCAGCAATGCCTGCTACATGGAGGAGATGCGCAACGTGGAACTGGTGGAAGGAGAAGAG GGGCGGATGTGTGTGAACATGGAATGGGGGGCCTTCGGGGACAATGGATGCCTAGATGACTTCCGCACAG AATTTGATGTGGCTGTGGATGAGCTTTCACTCAACCCCGGCAAGCAGAGGTTCGAGAAAATGATCAGTGG AATGTACCTGGGTGAGATTGTCCGTAACATTCTCATCGATTTCACCAAGCGTGGACTACTCTTCCGAGGC CGCATCTCAGAGCGGCTCAAGACAAGGGGCATCTTTGAAACCAAGTTCTTGTCTCAGATTGAGAGTGACT GCCTGGCCCTGCTGCAAGTCCGAGCCATCCTGCAACACTTAGGGCTTGAGAGCACCTGTGACGACAGCAT CATTGTTAAGGAGGTGTGCACTGTGGTGGCCCGGCGGGCAGCCCAGCTCTGTGGCGCAGGCATGGCCGCT GTGGTGGACAGGATACGAGAAAACCGTGGGCTGGACGCTCTCAAAGTGACAGTGGGTGTGGATGGGACCC TCTACAAGCTACATCCTCACTTTGCCAAAGTCATGCATGAGACAGTGAAGGACCTGGCTCCGAAATGTGA TGTGTCTTTCCTGCAGTCAGAGGATGGCAGCGGGAAGGGGGCGGCGCTCATCACTGCTGTGGCCTGCCGC ATCCGTGAGGCTGGACAGCGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209482 representing NM_000189
Red=Cloning site Green=Tags(s) MIASHLLAYFFTELNHDQVQKVDQYLYHMRLSDETLLEISKRFRKEMEKGLGATTHPTAAVKMLPTFVRS TPDGTEHGEFLALDLGGTNFRVLWVKVTDNGLQKVEMENQIYAIPEDIMRGSGTQLFDHIAECLANFMDK LQIKDKKLPLGFTFSFPCHQTKLDESFLVSWTKGFKSSGVEGRDVVALIRKAIQRRGDFDIDIVAVVNDT VGTMMTCGYDDHNCEIGLIVGTGSNACYMEEMRHIDMVEGDEGRMCINMEWGAFGDDGSLNDIRTEFDQE IDMGSLNPGKQLFEKMISGMYMGELVRLILVKMAKEELLFGGKLSPELLNTGRFETKDISDIEGEKDGIR KAREVLMRLGLDPTQEDCVATHRICQIVSTRSASLCAATLAAVLQRIKENKGEERLRSTIGVDGSVYKKH PHFAKRLHKTVRRLVPGCDVRFLRSEDGSGKGAAMVTAVAYRLADQHRARQKTLEHLQLSHDQLLEVKRR MKVEMERGLSKETHASAPVKMLPTYVCATPDGTEKGDFLALDLGGTNFRVLLVRVRNGKWGGVEMHNKIY AIPQEVMHGTGDELFDHIVQCIADFLEYMGMKGVSLPLGFTFSFPCQQNSLDESILLKWTKGFKASGCEG EDVVTLLKEAIHRREEFDLDVVAVVNDTVGTMMTCGFEDPHCEVGLIVGTGSNACYMEEMRNVELVEGEE GRMCVNMEWGAFGDNGCLDDFRTEFDVAVDELSLNPGKQRFEKMISGMYLGEIVRNILIDFTKRGLLFRG RISERLKTRGIFETKFLSQIESDCLALLQVRAILQHLGLESTCDDSIIVKEVCTVVARRAAQLCGAGMAA VVDRIRENRGLDALKVTVGVDGTLYKLHPHFAKVMHETVKDLAPKCDVSFLQSEDGSGKGAALITAVACR IREAGQR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000189 |
| ORF Size | 2751 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000189.5 |
| RefSeq Size | 7109 bp |
| RefSeq ORF | 2754 bp |
| Locus ID | 3099 |
| UniProt ID | P52789 |
| Domains | hexokinase |
| Protein Families | Druggable Genome |
| Protein Pathways | Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Metabolic pathways, Starch and sucrose metabolism, Type II diabetes mellitus |
| MW | 102.2 kDa |
| Gene Summary | Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009] |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Nuclear HKII-P-p53 (Ser15) Interaction is a Prognostic Biomarker for Chemoresponsiveness and Glycolytic Regulation in Epithelial Ovarian Cancer
,null,
Cancers
,PubMed ID 34298618
[Hexokinase II]
|
|
Hexokinase 2 Regulates Ovarian Cancer Cell Migration, Invasion and Stemness via FAK/ERK1/2/MMP9/NANOG/SOX9 Signaling Cascades
,Siu, MKY;Jiang, YX;Wang, JJ;Leung, THY;Han, CY;Tsang, BK;Cheung, ANY;Ngan, HYS;Chan, KKL;,
Cancers (Basel) 2019
,PubMed ID 31212816
[Hexokinase II]
|
|
Inhibition of S6K1 enhances dichloroacetate-induced cell death
,Hong, SE;Shin, KS;Lee, YH;Seo, SK;Yun, SM;Choe, TB;Kim, HA;Kim, EK;Noh, WC;Kim, JI;Hwang, CS;Lee, JK;Hwang, SG;Jin, HO;Park, IC;,
J. Cancer Res. Clin. Oncol.
,PubMed ID 25471732
[Hexokinase II]
|
|
Re-sensitization of 5-FU resistance by SPARC through negative regulation of glucose metabolism in hepatocellular carcinoma
,Hua, HW;Jiang, F;Huang, Q;Liao, ZJ;Ding, G;,
Tumour Biol.
,PubMed ID 25252848
[Hexokinase II]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209482L1 | Lenti ORF clone of Human hexokinase 2 (HK2), Myc-DDK-tagged |
CNY 12,472.00 |
|
| RC209482L2 | Lenti ORF clone of Human hexokinase 2 (HK2), mGFP tagged |
CNY 5,890.00 |
|
| RC209482L3 | Lenti ORF clone of Human hexokinase 2 (HK2), Myc-DDK-tagged |
CNY 12,472.00 |
|
| RC209482L4 | Lenti ORF clone of Human hexokinase 2 (HK2), mGFP tagged |
CNY 5,890.00 |
|
| RG209482 | HK2 (tGFP-tagged) - Human hexokinase 2 (HK2) |
CNY 11,672.00 |
|
| SC309063 | HK2 (untagged)-Human hexokinase 2 (HK2) |
CNY 10,080.00 |
其它Hexokinase II产品
