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PPP2R3A (NM_002718) Human Tagged ORF Clone
CAT＃: RC209027
- TrueORF®
PPP2R3A (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 1
ORF Plasmid: tGFP
"NM_002718" in other vectors (4)
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CNY 12,008.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PPP2R3; PR72; PR130 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209027 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCAACTTACAGACTTGTGGTTAGTACTGTGAACCACTACAGCAGCGTGGTGATAGACCGGCGTT TTGAACAAGCTATACATTATTGCACTGGAACCTGCCACACCTTCACACATGGAATTGACTGCATTGTGGT ACACCATAGTGTTTGTGCAGACCTCTTGCACATCCCTGTGTCTCAGTTCAAAGATGCAGATCTGAACTCT ATGTTTCTACCCCATGAAAATGGGCTTTCTTCGGCTGAAGGAGACTATCCCCAACAGGCCTTCACAGGCA TACCCAGGGTCAAGAGAGGATCTACATTTCAGAATACCTACAACTTAAAGGATATTGCAGGAGAAGCAAT CAGTTTTGCCAGTGGGAAAATAAAAGAATTTTCCTTTGAAAAACTCAAAAACTCTAACCATGCAGCTTAC AGAAAGGGAAGGAAAGTTAAGTCTGACTCATTTAATAGGAGGTCAGTTGATTTGGACTTGCTTTGTGGCC ATTATAACAACGATGGGAACGCCCCATCCTTTGGTTTACTGCGGAGTTCCTCAGTTGAGGAAAAACCTTT GTCTCATAGAAACTCACTGGATACGAACCTGACTTCCATGTTTCTTCAAAACTTTTCTGAAGAAGACTTG GTTACTCAGATTTTGGAAAAACATAAAATAGATAATTTTTCTTCTGGGACAGACATAAAGATGTGCTTGG ACATCTTATTGAAATGCTCCGAGGATTTAAAAAAATGCACAGACATCATAAAACAATGCATAAAGAAAAA ATCAGGGAGTAGCATCAGTGAAGGAAGTGGTAATGATACAATTTCTAGCTCTGAAACTGTCTATATGAAT GTAATGACCAGGTTAGCATCCTATCTGAAAAAGTTACCATTTGAATTCATGCAGTCTGGGAATAATGAGG CTCTAGATTTAACAGAACTGATCAGTAATATGCCTAGCTTACAACTGACTCCCTTCTCCCCAGTGTTTGG CACTGAACAACCCCCTAAATATGAAGATGTTGTCCAGCTCTCAGCTTCTGACTCTGGACGATTTCAAACT ATTGAATTGCAAAATGACAAGCCTAATTCTAGGAAGATGGACACTGTACAATCCATTCCAAACAACTCCA CAAATTCCTTATATAACTTAGAGGTAAATGATCCTAGAACTCTAAAAGCTGTCCAGGTCCAATCACAGTC ATTAACCATGAATCCTTTAGAAAATGTTTCTTCTGACGACTTAATGGAAACTCTTTATATTGAAGAAGAG TCAGATGGAAAGAAAGCATTAGATAAAGGACAAAAGACAGAGAATGGACCTAGTCATGAGTTATTAAAGG TAAATGAACATAGAGCAGAATTTCCAGAACATGCTACTCATCTTAAAAAATGCCCCACCCCAATGCAAAA TGAAATTGGTAAGATATTTGAGAAATCATTTGTTAATCTACCTAAGGAAGACTGTAAATCAAAAGTTTCT AAATTTGAAGAGGGAGACCAGAGAGATTTTACAAATTCCAGTAGCCAGGAAGAGATAGATAAATTGTTAA TGGATTTGGAATCTTTTTCACAGAAGATGGAGACCTCTCTAAGAGAGCCACTTGCGAAGGGTAAAAACTC TAATTTTTTAAATAGTCACAGTCAGTTGACCGGTCAGACCCTTGTAGATCTTGAGCCTAAATCTAAAGTC TCTTCACCCATAGAAAAAGTCTCACCTTCCTGTCTAACAAGGATTATTGAAACCAATGGACACAAAATAG AGGAAGAGGATCGAGCCCTCTTACTGCGAATCCTGGAAAGCATTGAAGACTTTGCTCAAGAACTAGTTGA ATGCAAATCAAGCAGAGGGAGCCTATCACAAGAAAAGGAAATGATGCAAATTCTACAGGAAACCTTGACA ACTTCCTCCCAGGCCAATTTATCAGTCTGTAGAAGTCCTGTTGGTGATAAAGCCAAAGATACTACTTCAG CAGTTTTGATTCAGCAGACTCCAGAGGTGATCAAGATTCAAAATAAACCAGAAAAGAAACCTGGAACACC ACTCCCACCTCCAGCCACCTCTCCAAGTAGTCCCCGACCTCTCTCCCCGGTTCCCCATGTGAATAATGTT GTGAATGCGCCATTGTCCATAAACATTCCACGGTTCTACTTTCCTGAAGGACTCCCAGATACCTGTAGTA ATCATGAACAAACTCTAAGCAGAATTGAAACTGCTTTCATGGATATTGAAGAACAGAAAGCAGACATTTA TGAAATGGGGAAAATTGCAAAGGTCTGTGGCTGTCCTCTCTATTGGAAAGCCCCCATGTTCAGGGCTGCA GGGGGAGAGAAGACAGGATTTGTGACAGCACAGTCATTCATTGCCATGTGGAGAAAGTTGCTGAATAACC ATCATGATGATGCCTCTAAATTCATCTGTCTTCTAGCAAAGCCCAACTGCAGCTCTCTAGAACAGGAGGA TTTCATCCCTCTACTTCAGGATGTGGTGGATACCCACCCTGGTCTCACGTTCCTGAAAGATGCTCCAGAA TTCCACTCCCGCTACATCACCACGGTTATTCAGAGAATATTCTACACAGTCAACAGATCTTGGAGTGGAA AAATTACTTCGACAGAGATAAGAAAAAGCAACTTTTTGCAAACCCTAGCACTTTTGGAAGAAGAGGAAGA TATAAACCAAATTACAGATTACTTCTCCTATGAACATTTCTATGTTATTTATTGTAAATTCTGGGAACTA GATACTGATCACGACCTCTACATCAGCCAGGCCGATCTGTCTCGATACAATGACCAGGCTTCATCAAGCA GGATTATTGAAAGGATATTCTCTGGTGCAGTAACAAGGGGAAAAACAATACAGAAAGAGGGAAGAATGAG CTATGCAGATTTTGTTTGGTTTTTGATCTCTGAAGAAGACAAAAGGAATCCTACCAGCATTGAGTATTGG TTCCGCTGCATGGATGTGGATGGAGACGGTGTACTCTCCATGTATGAGCTGGAGTACTTCTATGAGGAGC AGTGTGAACGGATGGAAGCCATGGGAATTGAGCCCTTGCCATTCCATGATTTACTGTGCCAGATGCTTGA CCTAGTGAAGCCAGCTGTTGATGGCAAAATAACTCTAAGAGATCTGAAGAGGTGCAGAATGGCTCACATC TTCTATGACACTTTCTTTAATCTGGAGAAATACTTAGACCATGAACAGAGAGATCCCTTTGCGGTCCAGA AGGATGTTGAGAACGATGGGCCTGAGCCCTCAGACTGGGACCGGTTTGCCGCTGAGGAGTATGAGACGCT TGTTGCAGAGGGATCTGCCCAAGCACAATTCCAGGAAGGCTTTGAAGATTATGAAACAGATGAACCTGCC TCTCCCTCTGAATTTGGAAACAAAAGCAATAAAATATTAAGTGCAAGCCTTCCAGAGAAATGTGGAAAGC TTCAATCAGTGGATGAAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209027 protein sequence
Red=Cloning site Green=Tags(s) MAATYRLVVSTVNHYSSVVIDRRFEQAIHYCTGTCHTFTHGIDCIVVHHSVCADLLHIPVSQFKDADLNS MFLPHENGLSSAEGDYPQQAFTGIPRVKRGSTFQNTYNLKDIAGEAISFASGKIKEFSFEKLKNSNHAAY RKGRKVKSDSFNRRSVDLDLLCGHYNNDGNAPSFGLLRSSSVEEKPLSHRNSLDTNLTSMFLQNFSEEDL VTQILEKHKIDNFSSGTDIKMCLDILLKCSEDLKKCTDIIKQCIKKKSGSSISEGSGNDTISSSETVYMN VMTRLASYLKKLPFEFMQSGNNEALDLTELISNMPSLQLTPFSPVFGTEQPPKYEDVVQLSASDSGRFQT IELQNDKPNSRKMDTVQSIPNNSTNSLYNLEVNDPRTLKAVQVQSQSLTMNPLENVSSDDLMETLYIEEE SDGKKALDKGQKTENGPSHELLKVNEHRAEFPEHATHLKKCPTPMQNEIGKIFEKSFVNLPKEDCKSKVS KFEEGDQRDFTNSSSQEEIDKLLMDLESFSQKMETSLREPLAKGKNSNFLNSHSQLTGQTLVDLEPKSKV SSPIEKVSPSCLTRIIETNGHKIEEEDRALLLRILESIEDFAQELVECKSSRGSLSQEKEMMQILQETLT TSSQANLSVCRSPVGDKAKDTTSAVLIQQTPEVIKIQNKPEKKPGTPLPPPATSPSSPRPLSPVPHVNNV VNAPLSINIPRFYFPEGLPDTCSNHEQTLSRIETAFMDIEEQKADIYEMGKIAKVCGCPLYWKAPMFRAA GGEKTGFVTAQSFIAMWRKLLNNHHDDASKFICLLAKPNCSSLEQEDFIPLLQDVVDTHPGLTFLKDAPE FHSRYITTVIQRIFYTVNRSWSGKITSTEIRKSNFLQTLALLEEEEDINQITDYFSYEHFYVIYCKFWEL DTDHDLYISQADLSRYNDQASSSRIIERIFSGAVTRGKTIQKEGRMSYADFVWFLISEEDKRNPTSIEYW FRCMDVDGDGVLSMYELEYFYEEQCERMEAMGIEPLPFHDLLCQMLDLVKPAVDGKITLRDLKRCRMAHI FYDTFFNLEKYLDHEQRDPFAVQKDVENDGPEPSDWDRFAAEEYETLVAEGSAQAQFQEGFEDYETDEPA SPSEFGNKSNKILSASLPEKCGKLQSVDEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002718 |
| ORF Size | 3450 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002718.5 |
| RefSeq Size | 6814 bp |
| RefSeq ORF | 3453 bp |
| Locus ID | 5523 |
| UniProt ID | Q06190 |
| Domains | EFh |
| Protein Families | Druggable Genome, Phosphatase |
| MW | 130.2 kDa |
| Gene Summary | This gene encodes one of the regulatory subunits of the protein phosphatase 2. Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2 holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B'' family. The B'' family has been further divided into subfamilies. The product of this gene belongs to the alpha subfamily of regulatory subunit B''. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Jun 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209027L3 | Lenti-ORF clone of PPP2R3A (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 1 |
CNY 10,260.00 |
|
| RC209027L4 | Lenti-ORF clone of PPP2R3A (mGFP-tagged)-Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 1 |
CNY 10,260.00 |
|
| RG209027 | PPP2R3A (tGFP-tagged) - Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 1 |
CNY 9,220.00 |
|
| SC125495 | PPP2R3A (untagged)-Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 1 |
CNY 8,016.00 |
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