C3orf33 (NM_173657) Human Tagged ORF Clone

CAT#: RC208973

  • TrueORF®

C3orf33 (Myc-DDK-tagged)-Human chromosome 3 open reading frame 33 (C3orf33)

ORF Plasmid: DDK tGFP



  "NM_173657" in other vectors (4)

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CNY 2,640.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-C3orf33 Antibody
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms AC3-33
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208973 representing NM_173657
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCATAGCTGGAATAATGTTACTTTTGAGAAGCATTCGACTGACATCAAAATTTACAAGCTCTTCGG
ATATTCCAGTAGAATTTATAAGAAGAAATGTTAAACTACGTGGACGATTACGCCGAATAACTGAGAATGG
TTTAGAAATTGAACATATACCTATTACTTTACCTATTATAGCTTCATTGAGAAAAGAGCCACGTGGTGCT
TTGCTGGTTAAGTTGGCTGGAGTAGAACTCGCTGAAACTGGGAAGGCATGGTTACAAAAAGAGCTAAAAC
CTTCCCAATTACTATGGTTCCAACTTCTTGGAAAGGAGAATTCAGCACTCTTTTGCTATCTTCTGGTGAG
TAAGGGTGGATATTTCAGCGTGAATCTGAATGAAGAAATTTTGAGAAGAGGCCTTGGCAAAACTGTTCTT
GTTAAAGGGCTTAAATATGATTCTAAAATCTACTGGACAGTTCACAGAAACTTACTTAAAGCTGAATTAA
CAGCCTTAAAAAAAGGAGAAGGAATATGGAAGGAAGACTCTGAAAAAGAAAGTTACTTAGAAAAATTCAA
AGATTCCTGGAGAGAAATATGGAAAAAGGACAGTTTTTTAAAAACAACAGGATCAGATTTCAGCTTGAAA
AAAGAAAGTTATTATGAAAAACTTAAAAGGACTTATGAAATATGGAAAGACAACATGAACAACTGCTCCT
TAATACTGAAGTTCAGAGAACTTATAAGTCGCATAAACTTTCGTAGAAAAGGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208973 representing NM_173657
Red=Cloning site Green=Tags(s)

MAIAGIMLLLRSIRLTSKFTSSSDIPVEFIRRNVKLRGRLRRITENGLEIEHIPITLPIIASLRKEPRGA
LLVKLAGVELAETGKAWLQKELKPSQLLWFQLLGKENSALFCYLLVSKGGYFSVNLNEEILRRGLGKTVL
VKGLKYDSKIYWTVHRNLLKAELTALKKGEGIWKEDSEKESYLEKFKDSWREIWKKDSFLKTTGSDFSLK
KESYYEKLKRTYEIWKDNMNNCSLILKFRELISRINFRRKG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_173657
ORF Size 753 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_173657.3
RefSeq Size 1931 bp
RefSeq ORF 756 bp
Locus ID 285315
UniProt ID Q6P1S2
MW 29.1 kDa
Gene Summary Isoform 2: Secreted protein may play a role in transcription regulation via the MAPK3/MAPK1 pathway through an unidentified receptor on the plasma membrane.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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