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CDA (NM_001785) Human Tagged ORF Clone
CAT＃: RC208922
CDA (Myc-DDK-tagged)-Human cytidine deaminase (CDA)
ORF Plasmid: tGFP
"NM_001785" in other vectors (6)
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CNY 1,800.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CDD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208922 representing NM_001785
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGAAGCGTCCTGCCTGCACCCTGAAGCCTGAGTGTGTCCAGCAGCTGCTGGTTTGCTCCCAGG AGGCCAAGAAGTCAGCCTACTGCCCCTACAGTCACTTTCCTGTGGGGGCTGCCCTGCTCACCCAGGAGGG GAGAATCTTCAAAGGGTGCAACATAGAAAATGCCTGCTACCCGCTGGGCATCTGTGCTGAACGGACCGCT ATCCAGAAGGCCGTCTCAGAAGGGTACAAGGATTTCAGGGCAATTGCTATCGCCAGTGACATGCAAGATG ATTTTATCTCTCCATGTGGGGCCTGCAGGCAAGTCATGAGAGAGTTTGGCACCAACTGGCCCGTGTACAT GACCAAGCCGGATGGTACGTATATTGTCATGACGGTCCAGGAGCTGCTGCCCTCCTCCTTTGGGCCTGAG GACCTGCAGAAGACTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208922 representing NM_001785
Red=Cloning site Green=Tags(s) MAQKRPACTLKPECVQQLLVCSQEAKKSAYCPYSHFPVGAALLTQEGRIFKGCNIENACYPLGICAERTA IQKAVSEGYKDFRAIAIASDMQDDFISPCGACRQVMREFGTNWPVYMTKPDGTYIVMTVQELLPSSFGPE DLQKTQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001785 |
| ORF Size | 438 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001785.1, NP_001776.1 |
| RefSeq Size | 892 bp |
| RefSeq ORF | 441 bp |
| Locus ID | 978 |
| UniProt ID | P32320 |
| Domains | dCMP_cyt_deam |
| Protein Families | Stem cell - Pluripotency |
| Protein Pathways | Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism |
| MW | 16 kDa |
| Gene Summary | This gene encodes an enzyme involved in pyrimidine salvaging. The encoded protein forms a homotetramer that catalyzes the irreversible hydrolytic deamination of cytidine and deoxycytidine to uridine and deoxyuridine, respectively. It is one of several deaminases responsible for maintaining the cellular pyrimidine pool. Mutations in this gene are associated with decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208922L1 | Lenti ORF clone of Human cytidine deaminase (CDA), Myc-DDK-tagged |
CNY 4,200.00 |
|
| RC208922L2 | Lenti ORF clone of Human cytidine deaminase (CDA), mGFP tagged |
CNY 5,890.00 |
|
| RC208922L3 | Lenti ORF clone of Human cytidine deaminase (CDA), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208922L4 | Lenti ORF clone of Human cytidine deaminase (CDA), mGFP tagged |
CNY 4,200.00 |
|
| RG208922 | CDA (tGFP-tagged) - Human cytidine deaminase (CDA) |
CNY 3,400.00 |
|
| SC119015 | CDA (untagged)-Human cytidine deaminase (CDA) |
CNY 1,800.00 |
