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MGAT4B (NM_014275) Human Tagged ORF Clone
CAT＃: RC208670
MGAT4B (Myc-DDK-tagged)-Human mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B (MGAT4B), transcript variant 1
ORF Plasmid: tGFP
"NM_014275" in other vectors (6)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GNT-IV; GNT-IVB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208670 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCTCCGCAATGGCACCTTCCTGACGCTGCTGCTCTTCTGCCTGTGCGCCTTCCTCTCGCTGTCCT GGTACGCGGCACTCAGCGGCCAGAAAGGCGACGTTGTGGACGTTTACCAGCGGGAGTTCCTGGCGCTGCG CGATCGGTTGCACGCAGCTGAGCAGGAGAGCCTGAAGCGCTCCAAGGAGCTCAACCTGGTGCTGGACGAG ATCAAGAGGGCCGTGTCAGAAAGGCAGGCGCTGCGAGACGGAGACGGCAATCGCACCTGGGGCCGCCTAA CAGAGGACCCCCGATTGAAGCCGTGGAACGGCTCACACCGGCACGTGCTGCACCTGCCCACCGTCTTCCA TCACCTGCCACACCTGCTGGCCAAGGAGAGCAGTCTGCAGCCCGCGGTGCGCGTGGGCCAGGGCCGCACC GGAGTGTCGGTGGTGATGGGCATCCCGAGCGTGCGGCGCGAGGTGCACTCGTACCTGACTGACACTCTGC ACTCGCTCATCTCCGAGCTGAGCCCGCAGGAGAAGGAGGACTCGGTCATCGTGGTGCTGATCGCCGAGAC TGACTCACAGTACACTTCGGCAGTGACAGAGAACATCAAGGCCTTGTTCCCCACGGAGATCCATTCTGGG CTCCTGGAGGTCATCTCACCCTCCCCCCACTTCTACCCTGACTTCTCCCGCCTCCGAGAGTCCTTTGGGG ACCCCAAGGAGAGAGTCAGGTGGAGGACCAAACAGAACCTCGATTACTGCTTCCTCATGATGTACGCGCA GTCCAAAGGCATCTACTACGTGCAGCTGGAGGATGACATCGTGGCCAAGCCCAACTACCTGAGCACCATG AAGAACTTTGCACTGCAGCAGCCTTCAGAGGACTGGATGATCCTGGAGTTCTCCCAGCTGGGCTTCATTG GTAAGATGTTCAAGTCGCTGGACCTGAGCCTGATTGTAGAGTTCATTCTCATGTTCTACCGGGACAAGCC CATCGACTGGCTCCTGGACCATATTCTGTGGGTGAAAGTCTGCAACCCCGAGAAGGATGCGAAGCACTGT GACCGGCAGAAAGCCAACCTGCGGATCCGCTTCAAACCGTCCCTCTTCCAGCACGTGGGCACTCACTCCT CGCTGGCTGGCAAGATCCAGAAACTGAAGGACAAAGACTTTGGAAAGCAGGCGCTGCGGAAGGAGCATGT GAACCCGCCAGCAGAGGTGAGCACGAGCCTGAAGACATACCAGCACTTCACCCTGGAGAAAGCCTACCTG CGCGAGGACTTCTTCTGGGCCTTCACCCCTGCCGCGGGGGACTTCATCCGCTTCCGCTTCTTCCAACCTC TAAGACTGGAGCGGTTCTTCTTCCGCAGTGGGAACATCGAGCACCCGGAGGACAAGCTCTTCAACACGTC TGTGGAGGTGCTGCCCTTCGACAACCCTCAGTCAGACAAGGAGGCCCTGCAGGAGGGCCGCACCGCCACC TTCCGGTACCCTCGGAGCCCCGACGGCTACCTCCAGATCGGCTCCTTCTACAAGGGAGTGGCAGAGGGAG AGGTGGACCCAGCCTTCGGCCCTCTGGAAGCACTGCGCCTCTCGATCCAGACGGACTCCCCTGTGTGGGT GATTCTGAGCGAGATCTTCCTGAAAAAGGCCGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208670 protein sequence
Red=Cloning site Green=Tags(s) MRLRNGTFLTLLLFCLCAFLSLSWYAALSGQKGDVVDVYQREFLALRDRLHAAEQESLKRSKELNLVLDE IKRAVSERQALRDGDGNRTWGRLTEDPRLKPWNGSHRHVLHLPTVFHHLPHLLAKESSLQPAVRVGQGRT GVSVVMGIPSVRREVHSYLTDTLHSLISELSPQEKEDSVIVVLIAETDSQYTSAVTENIKALFPTEIHSG LLEVISPSPHFYPDFSRLRESFGDPKERVRWRTKQNLDYCFLMMYAQSKGIYYVQLEDDIVAKPNYLSTM KNFALQQPSEDWMILEFSQLGFIGKMFKSLDLSLIVEFILMFYRDKPIDWLLDHILWVKVCNPEKDAKHC DRQKANLRIRFKPSLFQHVGTHSSLAGKIQKLKDKDFGKQALRKEHVNPPAEVSTSLKTYQHFTLEKAYL REDFFWAFTPAAGDFIRFRFFQPLRLERFFFRSGNIEHPEDKLFNTSVEVLPFDNPQSDKEALQEGRTAT FRYPRSPDGYLQIGSFYKGVAEGEVDPAFGPLEALRLSIQTDSPVWVILSEIFLKKAD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014275 |
| ORF Size | 1644 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014275.5 |
| RefSeq Size | 2482 bp |
| RefSeq ORF | 1647 bp |
| Locus ID | 11282 |
| UniProt ID | Q9UQ53 |
| Domains | Glyco_transf_55 |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
| MW | 63.2 kDa |
| Gene Summary | This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein, in addition to the related isoenzyme A, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208670L1 | Lenti ORF clone of Human mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B (MGAT4B), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208670L2 | Lenti ORF clone of Human mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B (MGAT4B), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC208670L3 | Lenti ORF clone of Human mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B (MGAT4B), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208670L4 | Lenti ORF clone of Human mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B (MGAT4B), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG208670 | MGAT4B (tGFP-tagged) - Human mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B (MGAT4B), transcript variant 1 |
CNY 4,370.00 |
|
| SC110257 | MGAT4B (untagged)-Human mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B (MGAT4B), transcript variant 1 |
CNY 2,950.00 |
