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SFRP5 (NM_003015) Human Tagged ORF Clone
CAT＃: RC208391
SFRP5 (Myc-DDK-tagged)-Human secreted frizzled-related protein 5 (SFRP5)
ORF Plasmid: tGFP
"NM_003015" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
| Cited in 3 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SARP3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208391 representing NM_003015
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGCGGCGGCGGCGGCGGGGGGCGTGCGGACGGCCGCGCTGGCGCTGCTGCTGGGGGCGCTGCACT GGGCGCCGGCGCGCTGCGAGGAGTACGACTACTATGGCTGGCAGGCCGAGCCGCTGCACGGCCGCTCCTA CTCCAAGCCGCCGCAGTGCCTTGACATCCCTGCCGACCTGCCGCTCTGCCACACGGTGGGCTACAAGCGC ATGCGGCTGCCCAACCTGCTGGAGCACGAGAGCCTGGCCGAAGTGAAGCAGCAGGCGAGCAGCTGGCTGC CGCTGCTGGCCAAGCGCTGCCACTCGGATACGCAGGTCTTCCTGTGCTCGCTCTTTGCGCCCGTCTGTCT CGACCGGCCCATCTACCCGTGCCGCTCGCTGTGCGAGGCCGTGCGCGCCGGCTGCGCGCCGCTCATGGAG GCCTACGGCTTCCCCTGGCCTGAGATGCTGCACTGCCACAAGTTCCCCCTGGACAACGACCTCTGCATCG CCGTGCAGTTCGGGCACCTGCCCGCCACCGCGCCTCCAGTGACCAAGATCTGCGCCCAGTGTGAGATGGA GCACAGTGCTGACGGCCTCATGGAGCAGATGTGCTCCAGTGACTTTGTGGTCAAAATGCGCATCAAGGAG ATCAAGATAGAGAATGGGGACCGGAAGCTGATTGGAGCCCAGAAAAAGAAGAAGCTGCTCAAGCCGGGCC CCCTGAAGCGCAAGGACACCAAGCGGCTGGTGCTGCACATGAAGAATGGCGCGGGCTGCCCCTGCCCACA GCTGGACAGCCTGGCGGGCAGCTTCCTGGTCATGGGCCGCAAAGTGGATGGACAGCTGCTGCTCATGGCC GTCTACCGCTGGGACAAGAAGAATAAGGAGATGAAGTTTGCAGTCAAATTCATGTTCTCCTACCCCTGCT CCCTCTACTACCCTTTCTTCTACGGGGCGGCAGAGCCCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208391 representing NM_003015
Red=Cloning site Green=Tags(s) MRAAAAAGGVRTAALALLLGALHWAPARCEEYDYYGWQAEPLHGRSYSKPPQCLDIPADLPLCHTVGYKR MRLPNLLEHESLAEVKQQASSWLPLLAKRCHSDTQVFLCSLFAPVCLDRPIYPCRSLCEAVRAGCAPLME AYGFPWPEMLHCHKFPLDNDLCIAVQFGHLPATAPPVTKICAQCEMEHSADGLMEQMCSSDFVVKMRIKE IKIENGDRKLIGAQKKKKLLKPGPLKRKDTKRLVLHMKNGAGCPCPQLDSLAGSFLVMGRKVDGQLLLMA VYRWDKKNKEMKFAVKFMFSYPCSLYYPFFYGAAEPH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003015 |
| ORF Size | 951 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003015.2, NP_003006.1 |
| RefSeq Size | 1905 bp |
| RefSeq ORF | 954 bp |
| Locus ID | 6425 |
| UniProt ID | Q5T4F7 |
| Protein Families | Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein |
| Protein Pathways | Wnt signaling pathway |
| MW | 35.4 kDa |
| Gene Summary | Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas. [provided by RefSeq, Jul 2008] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Secreted frizzled-related protein 5 suppresses aggressive phenotype and reverses docetaxel resistance in prostate cancer
,Xu, Q;Lü, Z;Wang, X;Zhu, Q;Wu, H;,
J. Investig. Med.
,PubMed ID 30787036
[SFRP5]
|
|
Secreted frizzled-related protein 5 protects against oxidative stress-induced apoptosis in human aortic endothelial cells via downregulation of Bax
,Wang, X;Peng, Q;Jiang, F;Xue, L;Li, J;Fan, Z;Chen, P;Chen, G;Cai, Y;,
J. Biochem. Mol. Toxicol.
,PubMed ID 28834606
[SFRP5]
|
|
Secreted frizzled-related protein-5 (sFRP-5) is epigenetically downregulated and functions as a tumor suppressor in kidney cancer
,null,
International journal of cancer. Journal international du cancer
,PubMed ID 20340127
[SFRP5]
|
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208391L1 | Lenti ORF clone of Human secreted frizzled-related protein 5 (SFRP5), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC208391L2 | Lenti ORF clone of Human secreted frizzled-related protein 5 (SFRP5), mGFP tagged |
CNY 5,890.00 |
|
| RC208391L3 | Lenti ORF clone of Human secreted frizzled-related protein 5 (SFRP5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208391L4 | Lenti ORF clone of Human secreted frizzled-related protein 5 (SFRP5), mGFP tagged |
CNY 5,890.00 |
|
| RG208391 | SFRP5 (tGFP-tagged) - Human secreted frizzled-related protein 5 (SFRP5) |
CNY 4,000.00 |
|
| SC125804 | SFRP5 (untagged)-Human secreted frizzled-related protein 5 (SFRP5) |
CNY 2,400.00 |
