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PPP2R2D (NM_018461) Human Tagged ORF Clone
CAT＃: RC208230
- TrueORF®
PPP2R2D (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B, delta (PPP2R2D), transcript variant 1
ORF Plasmid: tGFP
"NM_018461" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | B55D; B55delta; MDS026 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208230 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGAGCCGGAGGCGGCGGCTGCCCCGCGGGCGGCAACGACTTCCAGTGGTGCTTCTCGCAGGTCA AGGGGGCCATCGACGAGGACGTGGCCGAAGCGGACATCATTTCCACCGTTGAGTTTAATTACTCTGGAGA TCTTCTTGCAACAGGAGACAAGGGCGGCAGAGTTGTTATTTTTCAGCGTGAACAAGAGAATAAAAGCCGC CCTCATTCTAGGGGAGAATATAATGTTTACAGCACCTTTCAAAGTCATGAACCGGAGTTTGACTATTTGA AAAGTCTAGAAATTGAGGAAAAAATTAATAAAATTAGGTGGTTACCACAACAGAATGCTGCTCATTTTCT ACTGTCTACAAATGATAAAACTATAAAATTATGGAAAATAAGTGAACGGGATAAAAGAGCAGAAGGTTAT AACCTGAAAGACGAAGATGGAAGACTTCGAGACCCATTTAGGATCACGGCGCTACGGGTCCCAATATTGA AGCCCATGGATCTTATGGTAGAAGCGAGTCCACGGCGAATTTTTGCAAATGCTCACACATATCATATAAA TTCCATTTCAGTAAATAGTGATCATGAAACATATCTTTCTGCAGATGACCTGAGAATTAATTTATGGCAC TTAGAAATCACAGATAGAAGCTTTAACATCGTGGACATCAAGCCTGCTAACATGGAGGAGCTGACCGAAG TCATCACTGCAGCCGAGTTCCACCCGCACCAGTGCAACGTGTTCGTCTACAGCAGTAGCAAAGGGACCAT CCGCCTGTGTGACATGCGCTCCTCGGCCCTGTGCGACAGACACTCCAAGTTTTTTGAAGAGCCTGAAGAT CCCAGCAGTAGGTCCTTCTTCTCAGAAATAATTTCATCCATATCCGATGTAAAATTCAGTCATAGTGGGC GGTACATGATGACCAGAGACTACCTGTCGGTGAAGGTGTGGGACCTCAACATGGAGAGCAGGCCGGTGGA GACCCACCAGGTCCACGAGTACCTGCGCAGCAAGCTCTGCTCTCTCTATGAGAACGACTGCATCTTTGAC AAGTTTGAGTGTTGCTGGAACGGTTCGGATAGCGCCATCATGACCGGGTCCTATAACAACTTCTTCAGGA TGTTTGATAGAGACACGCGGAGGGATGTGACCCTGGAGGCCTCGAGAGAGAGCAGCAAACCGCGCGCCAG CCTCAAACCCCGGAAGGTGTGTACGGGGGGTAAGCGGAGGAAAGACGAGATCAGTGTGGACAGTCTGGAC TTCAACAAGAAGATCCTGCACACAGCCTGGCACCCCGTGGACAATGTCATTGCCGTGGCTGCCACCAATA ACTTGTACATATTCCAGGACAAAATCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208230 protein sequence
Red=Cloning site Green=Tags(s) MAGAGGGGCPAGGNDFQWCFSQVKGAIDEDVAEADIISTVEFNYSGDLLATGDKGGRVVIFQREQENKSR PHSRGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQQNAAHFLLSTNDKTIKLWKISERDKRAEGY NLKDEDGRLRDPFRITALRVPILKPMDLMVEASPRRIFANAHTYHINSISVNSDHETYLSADDLRINLWH LEITDRSFNIVDIKPANMEELTEVITAAEFHPHQCNVFVYSSSKGTIRLCDMRSSALCDRHSKFFEEPED PSSRSFFSEIISSISDVKFSHSGRYMMTRDYLSVKVWDLNMESRPVETHQVHEYLRSKLCSLYENDCIFD KFECCWNGSDSAIMTGSYNNFFRMFDRDTRRDVTLEASRESSKPRASLKPRKVCTGGKRRKDEISVDSLD FNKKILHTAWHPVDNVIAVAATNNLYIFQDKIN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018461 |
| ORF Size | 1359 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018461.5 |
| RefSeq Size | 5754 bp |
| RefSeq ORF | 1362 bp |
| Locus ID | 55844 |
| UniProt ID | Q66LE6 |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | Tight junction |
| MW | 52 kDa |
| Gene Summary | B regulatory subunit of protein phosphatase 2A (PP2A) that plays a key role in cell cycle by controlling mitosis entry and exit. The activity of PP2A complexes containing PPP2R2D (PR55-delta) fluctuate during the cell cycle: the activity is high in interphase and low in mitosis. During mitosis, activity of PP2A is inhibited via interaction with phosphorylated ENSA and ARPP19 inhibitors. Within the PP2A complexes, the B regulatory subunits modulate substrate selectivity and catalytic activity, and also may direct the localization of the catalytic enzyme to a particular subcellular compartment (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Dephosphorylation of Plk1 occurs through PP2A-B55/ENSA/Greatwall pathway during mitotic DNA damage recovery
,Kim, SY;Hyun, SY;Jang, YJ;,
Cell Cycle
,PubMed ID 31072185
[PPP2R2D]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208230L3 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B, delta (PPP2R2D), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208230L4 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B, delta (PPP2R2D), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG208230 | PPP2R2D (tGFP-tagged) - Human protein phosphatase 2, regulatory subunit B, delta (PPP2R2D), transcript variant 1 |
CNY 4,370.00 |
|
| SC128054 | PPP2R2D (untagged)-Human protein phosphatase 2, regulatory subunit B, delta (PPP2R2D), transcript variant 1 |
CNY 7,220.00 |
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