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PDP1 (NM_018444) Human Tagged ORF Clone
CAT＃: RC208198
PDP1 (Myc-DDK-tagged)-Human pyruvate dehyrogenase phosphatase catalytic subunit 1 (PDP1), nuclear gene encoding mitochondrial protein, transcript variant 5
ORF Plasmid: tGFP
"NM_018444" in other vectors (6)
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CNY 3,990.00
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PDH; PDP; PDPC; PPM2A; PPM2C |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208198 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCACCAACTCAACTGTTTTTTCCTCTCATCCGTAACTGTGAACTGAGCAGGATCTATGGCACTG CATGTTACTGCCACCACAAACATCTCTGTTGTTCCTCATCGTACATTCCTCAGAGTCGACTGAGATACAC ACCTCATCCAGCATATGCTACCTTTTGCAGGCCAAAGGAGAACTGGTGGCAGTACACCCAAGGAAGGAGA TATGCTTCCACACCACAGAAATTTTACCTCACACCTCCACAAGTCAATAGCATCCTTAAAGCTAATGAAT ACAGTTTCAAAGTGCCAGAATTTGACGGCAAAAATGTCAGTTCTATCCTTGGATTTGACAGCAATCAGCT GCCTGCAAATGCACCCATTGAGGACCGGAGAAGTGCAGCAACCTGCTTGCAGACCAGAGGGATGCTTTTG GGGGTTTTTGATGGCCATGCAGGTTGTGCTTGTTCCCAGGCAGTCAGTGAAAGACTCTTTTATTATATTG CTGTCTCTTTGTTACCCCATGAGACTTTGCTAGAGATTGAAAATGCAGTGGAGAGCGGCCGGGCACTGCT ACCCATTCTCCAGTGGCACAAGCACCCCAATGATTACTTTAGTAAGGAGGCATCCAAATTGTACTTTAAC AGCTTGAGGACTTACTGGCAAGAGCTTATAGACCTCAACACTGGTGAGTCGACTGATATTGATGTTAAGG AGGCTCTAATTAATGCCTTCAAGAGGCTTGATAATGACATCTCCTTGGAGGCGCAAGTTGGTGATCCTAA TTCTTTTCTCAACTACCTGGTGCTTCGAGTGGCATTTTCTGGAGCCACTGCTTGTGTGGCCCATGTGGAT GGTGTTGACCTTCATGTGGCCAATACTGGCGATAGCAGAGCCATGCTGGGTGTGCAGGAAGAGGACGGCT CATGGTCAGCAGTCACGCTGTCTAATGACCACAATGCTCAAAATGAAAGAGAACTAGAACGGCTGAAATT GGAACATCCAAAGAGTGAGGCCAAGAGTGTCGTGAAACAGGATCGGCTGCTTGGCTTGCTGATGCCATTT AGGGCATTTGGAGATGTAAAGTTCAAATGGAGCATTGACCTTCAAAAGAGAGTGATAGAATCTGGCCCAG ACCAGTTGAATGACAATGAATATACCAAGTTTATTCCTCCTAATTATCACACACCTCCTTATCTCACTGC TGAGCCAGAGGTAACTTACCACCGATTAAGGCCACAGGATAAGTTTCTGGTGTTGGCTACTGATGGGTTG TGGGAGACTATGCATAGGCAGGATGTGGTTAGGATTGTGGGTGAGTACCTAACTGGCATGCATCACCAAC AGCCAATAGCTGTTGGTGGCTACAAGGTGACTCTGGGACAGATGCATGGCCTTTTAACAGAAAGGAGAAC CAAAATGTCCTCGGTATTTGAGGATCAGAACGCAGCAACCCATCTCATTCGCCACGCTGTGGGCAACAAC GAGTTTGGGACTGTTGATCATGAGCGCCTCTCTAAAATGCTTAGTCTTCCTGAAGAGCTTGCTCGAATGT ACAGAGATGACATTACAATCATTGTAGTTCAGTTCAATTCTCATGTTGTAGGGGCGTATCAAAACCAAGA A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208198 protein sequence
Red=Cloning site Green=Tags(s) MPAPTQLFFPLIRNCELSRIYGTACYCHHKHLCCSSSYIPQSRLRYTPHPAYATFCRPKENWWQYTQGRR YASTPQKFYLTPPQVNSILKANEYSFKVPEFDGKNVSSILGFDSNQLPANAPIEDRRSAATCLQTRGMLL GVFDGHAGCACSQAVSERLFYYIAVSLLPHETLLEIENAVESGRALLPILQWHKHPNDYFSKEASKLYFN SLRTYWQELIDLNTGESTDIDVKEALINAFKRLDNDISLEAQVGDPNSFLNYLVLRVAFSGATACVAHVD GVDLHVANTGDSRAMLGVQEEDGSWSAVTLSNDHNAQNERELERLKLEHPKSEAKSVVKQDRLLGLLMPF RAFGDVKFKWSIDLQKRVIESGPDQLNDNEYTKFIPPNYHTPPYLTAEPEVTYHRLRPQDKFLVLATDGL WETMHRQDVVRIVGEYLTGMHHQQPIAVGGYKVTLGQMHGLLTERRTKMSSVFEDQNAATHLIRHAVGNN EFGTVDHERLSKMLSLPEELARMYRDDITIIVVQFNSHVVGAYQNQE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018444 |
| ORF Size | 1611 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018444.4 |
| RefSeq Size | 4291 bp |
| RefSeq ORF | 1614 bp |
| Locus ID | 54704 |
| UniProt ID | Q9P0J1 |
| Domains | PP2C |
| Protein Families | Druggable Genome, Phosphatase |
| MW | 61.1 kDa |
| Gene Summary | Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208198L1 | Lenti ORF clone of Human pyruvate dehyrogenase phosphatase catalytic subunit 1 (PDP1), nuclear gene encoding mitochondrial protein, transcript variant 5, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208198L2 | Lenti ORF clone of Human pyruvate dehyrogenase phosphatase catalytic subunit 1 (PDP1), nuclear gene encoding mitochondrial protein, transcript variant 5, mGFP tagged |
CNY 5,890.00 |
|
| RC208198L3 | Lenti ORF clone of Human pyruvate dehyrogenase phosphatase catalytic subunit 1 (PDP1), nuclear gene encoding mitochondrial protein, transcript variant 5, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208198L4 | Lenti ORF clone of Human pyruvate dehyrogenase phosphatase catalytic subunit 1 (PDP1), nuclear gene encoding mitochondrial protein, transcript variant 5, mGFP tagged |
CNY 5,890.00 |
|
| RG208198 | PDP1 (tGFP-tagged) - Human pyruvate dehyrogenase phosphatase catalytic subunit 1 (PDP1), nuclear gene encoding mitochondrial protein, transcript variant 5 |
CNY 4,370.00 |
|
| SC128049 | PDP1 (untagged)-Human pyruvate dehyrogenase phosphatase catalytic subunit 1 (PDP1), nuclear gene encoding mitochondrial protein, transcript variant 5 |
CNY 2,950.00 |
