United States
Germany
Japan
United Kingdom
China
ACSL3 (NM_004457) Human Tagged ORF Clone
CAT＃: RC208032
ACSL3 (Myc-DDK-tagged)-Human acyl-CoA synthetase long-chain family member 3 (ACSL3), transcript variant 1
ORF Plasmid: tGFP
"NM_004457" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ACS3; FACL3; LACS 3; LACS3; PRO2194 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208032 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATAACCACGTGTCTTCAAAACCATCTACCATGAAGCTAAAACATACCATCAACCCTATTCTTTTAT ATTTTATACATTTTCTAATATCACTTTATACTATTTTAACATACATTCCGTTTTATTTTTTCTCCGAGTC AAGACAAGAAAAATCAAACCGAATTAAAGCAAAGCCTGTAAATTCAAAACCTGATTCTGCATACAGATCT GTTAATAGTTTGGATGGTTTGGCTTCAGTATTATACCCTGGATGTGATACTTTAGATAAAGTTTTTACAT ATGCAAAAAACAAATTTAAGAACAAAAGACTCTTGGGAACACGTGAAGTTTTAAATGAGGAAGATGAAGT ACAACCAAATGGAAAAATTTTTAAAAAGGTTATTCTTGGACAGTATAATTGGCTTTCCTATGAAGATGTC TTTGTTCGAGCCTTTAATTTTGGAAATGGATTACAGATGTTGGGTCAGAAACCAAAGACCAACATCGCCA TCTTCTGTGAGACCAGGGCCGAGTGGATGATAGCTGCACAGGCGTGTTTTATGTATAATTTTCAGCTTGT TACATTATATGCCACTCTAGGAGGTCCAGCCATTGTTCATGCATTAAATGAAACAGAGGTGACCAACATC ATTACTAGTAAAGAACTCTTACAAACAAAGTTGAAGGATATAGTTTCTTTGGTCCCACGCCTGCGGCACA TCATCACTGTTGATGGAAAGCCACCGACCTGGTCCGAGTTCCCCAAGGGCATCATTGTGCATACCATGGC TGCAGTGGAGGCCCTGGGAGCCAAGGCCAGCATGGAAAACCAACCTCATAGCAAACCATTGCCCTCAGAT ATTGCAGTAATCATGTACACAAGTGGATCCACAGGACTTCCAAAGGGAGTCATGATCTCACATAGTAACA TTATTGCTGGTATAACTGGGATGGCAGAAAGGATTCCAGAACTAGGAGAGGAAGATGTCTACATTGGATA TTTGCCTCTGGCCCATGTTCTAGAATTAAGTGCTGAGCTTGTCTGTCTTTCTCACGGATGCCGCATTGGT TACTCTTCACCACAGACTTTAGCAGATCAGTCTTCAAAAATTAAAAAAGGAAGCAAAGGGGATACATCCA TGTTGAAACCAACACTGATGGCAGCAGTTCCGGAAATCATGGATCGGATCTACAAAAATGTCATGAATAA AGTCAGTGAAATGAGTAGTTTTCAACGTAATCTGTTTATTCTGGCCTATAATTACAAAATGGAACAGATT TCAAAAGGACGTAATACTCCACTGTGCGACAGCTTTGTTTTCCGGAAAGTTCGAAGCTTGCTAGGGGGAA ATATTCGTCTCCTGTTGTGTGGTGGCGCTCCACTTTCTGCAACCACGCAGCGATTCATGAACATCTGTTT CTGCTGTCCTGTTGGTCAGGGATACGGGCTCACTGAATCTGCTGGGGCTGGAACAATTTCCGAAGTGTGG GACTACAATACTGGCAGAGTGGGAGCACCATTAGTTTGCTGTGAAATCAAATTAAAAAACTGGGAGGAAG GTGGATACTTTAATACTGATAAGCCACACCCCAGGGGTGAAATTCTTATTGGGGGCCAAAGTGTGACAAT GGGGTACTATAAAAATGAAGCAAAAACAAAAGCTGATTTCTTTGAAGATGAAAATGGACAAAGGTGGCTC TGTACTGGGGATATTGGAGAGTTTGAACCCGATGGATGCTTAAAGATTATTGATCGTAAAAAGGACCTTG TAAAACTACAGGCAGGGGAATATGTTTCTCTTGGGAAAGTAGAGGCAGCTTTGAAGAATCTTCCACTAGT AGATAACATTTGTGCATATGCAAACAGTTATCATTCTTATGTCATTGGATTTGTTGTGCCAAATCAAAAG GAACTAACTGAACTAGCTCGAAAGAAAGGACTTAAAGGGACTTGGGAGGAGCTGTGTAACAGTTGTGAAA TGGAAAATGAGGTACTTAAAGTGCTTTCCGAAGCTGCTATTTCAGCAAGTCTGGAAAAGTTTGAAATTCC AGTAAAAATTCGTTTGAGTCCTGAACCGTGGACCCCTGAAACTGGTCTGGTGACAGATGCCTTCAAGCTG AAACGCAAAGAGCTTAAAACACATTACCAGGCGGACATTGAGCGAATGTATGGAAGAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208032 protein sequence
Red=Cloning site Green=Tags(s) MNNHVSSKPSTMKLKHTINPILLYFIHFLISLYTILTYIPFYFFSESRQEKSNRIKAKPVNSKPDSAYRS VNSLDGLASVLYPGCDTLDKVFTYAKNKFKNKRLLGTREVLNEEDEVQPNGKIFKKVILGQYNWLSYEDV FVRAFNFGNGLQMLGQKPKTNIAIFCETRAEWMIAAQACFMYNFQLVTLYATLGGPAIVHALNETEVTNI ITSKELLQTKLKDIVSLVPRLRHIITVDGKPPTWSEFPKGIIVHTMAAVEALGAKASMENQPHSKPLPSD IAVIMYTSGSTGLPKGVMISHSNIIAGITGMAERIPELGEEDVYIGYLPLAHVLELSAELVCLSHGCRIG YSSPQTLADQSSKIKKGSKGDTSMLKPTLMAAVPEIMDRIYKNVMNKVSEMSSFQRNLFILAYNYKMEQI SKGRNTPLCDSFVFRKVRSLLGGNIRLLLCGGAPLSATTQRFMNICFCCPVGQGYGLTESAGAGTISEVW DYNTGRVGAPLVCCEIKLKNWEEGGYFNTDKPHPRGEILIGGQSVTMGYYKNEAKTKADFFEDENGQRWL CTGDIGEFEPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPLVDNICAYANSYHSYVIGFVVPNQK ELTELARKKGLKGTWEELCNSCEMENEVLKVLSEAAISASLEKFEIPVKIRLSPEPWTPETGLVTDAFKL KRKELKTHYQADIERMYGRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004457 |
| ORF Size | 2160 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004457.5 |
| RefSeq Size | 4369 bp |
| RefSeq ORF | 2163 bp |
| Locus ID | 2181 |
| UniProt ID | O95573 |
| Domains | AMP-binding |
| Protein Families | Transmembrane |
| Protein Pathways | Adipocytokine signaling pathway, Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway |
| MW | 80.4 kDa |
| Gene Summary | The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
TNF-α induces acyl-CoA synthetase 3 to promote lipid droplet formation in human endothelial cells
,Jung, HS;Shimizu-Albergine, M;Shen, X;Kramer, F;Shao, D;Vivekanandan-Giri, A;Pennathur, S;Tian, R;Kanter, JE;Bornfeldt, KE;,
J. Lipid Res.
,PubMed ID 31722970
[ACSL3]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208032L1 | Lenti ORF clone of Human acyl-CoA synthetase long-chain family member 3 (ACSL3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208032L2 | Lenti ORF clone of Human acyl-CoA synthetase long-chain family member 3 (ACSL3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC208032L3 | Lenti ORF clone of Human acyl-CoA synthetase long-chain family member 3 (ACSL3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208032L4 | Lenti ORF clone of Human acyl-CoA synthetase long-chain family member 3 (ACSL3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG208032 | ACSL3 (tGFP-tagged) - Human acyl-CoA synthetase long-chain family member 3 (ACSL3), transcript variant 1 |
CNY 4,370.00 |
|
| SC117368 | ACSL3 (untagged)-Human acyl-CoA synthetase long-chain family member 3 (ACSL3), transcript variant 1 |
CNY 2,950.00 |
