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TRAIL (TNFSF10) (NM_003810) Human Tagged ORF Clone
CAT＃: RC207596
TNFSF10 (Myc-DDK-tagged)-Human tumor necrosis factor (ligand) superfamily, member 10 (TNFSF10), transcript variant 1
ORF Plasmid: tGFP
"NM_003810" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Apo-2L; APO2L; CD253; TL2; TNLG6A; TRAIL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207596 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTATGATGGAGGTCCAGGGGGGACCCAGCCTGGGACAGACCTGCGTGCTGATCGTGATCTTCACAG TGCTCCTGCAGTCTCTCTGTGTGGCTGTAACTTACGTGTACTTTACCAACGAGCTGAAGCAGATGCAGGA CAAGTACTCCAAAAGTGGCATTGCTTGTTTCTTAAAAGAAGATGACAGTTATTGGGACCCCAATGACGAA GAGAGTATGAACAGCCCCTGCTGGCAAGTCAAGTGGCAACTCCGTCAGCTCGTTAGAAAGATGATTTTGA GAACCTCTGAGGAAACCATTTCTACAGTTCAAGAAAAGCAACAAAATATTTCTCCCCTAGTGAGAGAAAG AGGTCCTCAGAGAGTAGCAGCTCACATAACTGGGACCAGAGGAAGAAGCAACACATTGTCTTCTCCAAAC TCCAAGAATGAAAAGGCTCTGGGCCGCAAAATAAACTCCTGGGAATCATCAAGGAGTGGGCATTCATTCC TGAGCAACTTGCACTTGAGGAATGGTGAACTGGTCATCCATGAAAAAGGGTTTTACTACATCTATTCCCA AACATACTTTCGATTTCAGGAGGAAATAAAAGAAAACACAAAGAACGACAAACAAATGGTCCAATATATT TACAAATACACAAGTTATCCTGACCCTATATTGTTGATGAAAAGTGCTAGAAATAGTTGTTGGTCTAAAG ATGCAGAATATGGACTCTATTCCATCTATCAAGGGGGAATATTTGAGCTTAAGGAAAATGACAGAATTTT TGTTTCTGTAACAAATGAGCACTTGATAGACATGGACCATGAAGCCAGTTTTTTCGGGGCCTTTTTAGTT GGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207596 protein sequence
Red=Cloning site Green=Tags(s) MAMMEVQGGPSLGQTCVLIVIFTVLLQSLCVAVTYVYFTNELKQMQDKYSKSGIACFLKEDDSYWDPNDE ESMNSPCWQVKWQLRQLVRKMILRTSEETISTVQEKQQNISPLVRERGPQRVAAHITGTRGRSNTLSSPN SKNEKALGRKINSWESSRSGHSFLSNLHLRNGELVIHEKGFYYIYSQTYFRFQEEIKENTKNDKQMVQYI YKYTSYPDPILLMKSARNSCWSKDAEYGLYSIYQGGIFELKENDRIFVSVTNEHLIDMDHEASFFGAFLV G TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003810 |
| ORF Size | 843 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003810.2, NP_003801.1 |
| RefSeq Size | 1953 bp |
| RefSeq ORF | 846 bp |
| Locus ID | 8743 |
| UniProt ID | P50591 |
| Domains | TNF |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Apoptosis, Cytokine-cytokine receptor interaction, Natural killer cell mediated cytotoxicity |
| MW | 32.5 kDa |
| Gene Summary | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Evaluation of Efficiency of Modified Polypropylenimine (PPI) with Alkyl Chains as Non-viral Vectors Used in Co-delivery of Doxorubicin and TRAIL Plasmid
,Ebrahimian, M;Taghavi, S;Ghoreishi, M;Sedghi, S;Amel Farzad, S;Ramezani, M;Hashemi, M;,
AAPS PharmSciTech
,PubMed ID 29116619
[TRAIL]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207596L1 | Lenti ORF clone of Human tumor necrosis factor (ligand) superfamily, member 10 (TNFSF10), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC207596L2 | Lenti ORF clone of Human tumor necrosis factor (ligand) superfamily, member 10 (TNFSF10), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC207596L3 | Lenti ORF clone of Human tumor necrosis factor (ligand) superfamily, member 10 (TNFSF10), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC207596L4 | Lenti ORF clone of Human tumor necrosis factor (ligand) superfamily, member 10 (TNFSF10), transcript variant 1, mGFP tagged |
CNY 4,800.00 |
|
| RG207596 | TNFSF10 (tGFP-tagged) - Human tumor necrosis factor (ligand) superfamily, member 10 (TNFSF10), transcript variant 1 |
CNY 4,000.00 |
|
| SC321920 | TNFSF10 (untagged)-Human tumor necrosis factor (ligand) superfamily, member 10 (TNFSF10), transcript variant 1 |
CNY 2,400.00 |
