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Apc2 (ANAPC2) (NM_013366) Human Tagged ORF Clone
CAT＃: RC207539
ANAPC2 (Myc-DDK-tagged)-Human anaphase promoting complex subunit 2 (ANAPC2)
ORF Plasmid: tGFP
"NM_013366" in other vectors (7)
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CNY 5,800.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | APC2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207539 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCAGTTGTGGTGGCGGAGGGGGACAGCGACTCCCGGCCCGGACAGGAGTTGTTAGTGGCCT GGAACACCGTGAGCACCGGCCTGGTGCCGCCGGCTGCGCTGGGGCTGGTGTCTTCCCGGACCAGCGGTGC AGTCCCGCCAAAGGAAGAGGAGCTCCGGGCGGCGGTGGAGGTTCTGAGGGGCCACGGGCTACACTCGGTC CTGGAGGAGTGGTTCGTGGAGGTGCTGCAGAACGATCTGCAGGCCAACATCTCCCCTGAGTTCTGGAATG CCATCTCCCAATGCGAGAACTCTGCGGATGAGCCCCAGTGCCTTTTGCTACTCCTTGACGCTTTTGGCCT GCTGGAGAGCCGCCTGGATCCCTACCTGCGTAGCCTAGAGCTGCTGGAGAAATGGACTCGCCTGGGCTTG CTGATGGGCACTGGTGCTCAGGGGCTGCGAGAAGAAGTCCACACTATGTTGCGCGGAGTCTTGTTCTTTA GCACCCCCAGAACCTTCCAAGAGATGATCCAGCGTCTGTATGGGTGCTTCTTGAGAGTCTATATGCAGAG TAAGAGGAAGGGGGAAGGGGGCACAGACCCGGAACTGGAAGGGGAGCTGGACAGCCGGTATGCCCGTCGC CGGTACTACCGGCTCCTGCAGAGCCCGCTGTGTGCAGGGTGCAGCAGTGACAAGCAACAGTGCTGGTGTC GCCAGGCTCTGGAGCAGTTCCATCAGCTCAGCCAGGTCTTACACAGGCTCAGTCTGCTGGAGCGGGTCAG TGCCGAGGCTGTGACCACCACCCTGCACCAGGTGACCCGGGAGAGGATGGAGGACCGTTGCCGGGGCGAG TACGAGCGCTCCTTCCTGCGTGAGTTCCACAAGTGGATCGAGCGGGTGGTCGGCTGGCTCGGCAAGGTGT TCCTGCAGGACGGCCCCGCCAGGCCCGCATCTCCCGAGGCCGGCAACACCCTGCGCCGCTGGCGCTGCCA CGTGCAAAGGTTCTTCTACCGCATCTACGCCAGCCTGCGCATCGAGGAGCTCTTCAGCATCGTCCGAGAC TTCCCAGACTCCCGGCCAGCCATCGAGGACCTCAAGTACTGCCTGGAGAGGACGGACCAGAGGCAGCAGC TGCTCGTGTCCCTCAAGGCTGCCCTGGAGACTCGGCTCCTGCATCCAGGCGTCAACACGTGTGACATCAT CACCCTCTATATCTCTGCCATCAAGGCGCTGCGCGTGCTGGACCCTTCCATGGTCATCCTGGAGGTGGCC TGTGAGCCTATCCGCCGCTACCTGAGGACGCGGGAGGACACAGTGCGGCAGATTGTGGCTGGGCTGACGG GGGACTCGGACGGGACAGGGGACCTGGCTGTTGAGCTGTCCAAGACCGACCCGGCGAGCCTGGAGACAGG CCAGGACAGTGAGGATGACTCAGGCGAGCCAGAGGACTGGGTCCCGGACCCTGTGGATGCCGATCCAGGG AAGTCGAGCTCCAAGCGGCGTTCATCGGACATCATCAGCCTGCTGGTCAGCATCTACGGCAGCAAGGACC TCTTCATCAATGAGTACCGCTCGCTGCTGGCCGACCGCCTGCTGCACCAGTTCAGCTTCAGCCCCGAGCG GGAGATCCGCAACGTGGAGCTGCTGAAGCTGCGCTTTGGCGAGGCCCCAATGCACTTCTGTGAAGTCATG CTGAAGGACATGGCGGACTCCCGCCGCATCAATGCCAACATCCGGGAGGAGGATGAGAAGCGGCCAGCAG AGGAGCAGCCACCGTTCGGGGTCTACGCTGTCATCCTGTCCAGTGAGTTCTGGCCGCCCTTCAAGGACGA GAAGCTGGAGGTCCCCGAGGATATCAGGGCAGCCCTGGAGGCTTACTGCAAGAAGTATGAGCAGCTCAAG GCCATGCGGACCCTCAGTTGGAAGCACACCCTGGGCCTGGTGACCATGGACGTGGAGCTGGCCGACCGCA CGCTGTCTGTGGCGGTCACCCCAGTACAGGCGGTGATCTTGCTGTATTTTCAGGACCAAGCCAGCTGGAC CCTGGAGGAACTGAGCAAGGCGGTGAAGATGCCCGTGGCGCTGCTGCGGCGGCGGATGTCCGTGTGGCTG CAGCAGGGTGTGCTGCGTGAGGAGCCCCCCGGCACCTTCTCTGTCATTGAGGAGGAGCGGCCTCAGGACC GGGACAACATGGTGCTCATTGACAGTGACGACGAGAGCGACTCCGGCATGGCCTCCCAGGCCGACCAGAA GGAGGAGGAGCTGCTGCTCTTCTGGACGTACATCCAGGCCATGCTGACCAACCTGGAGAGCCTCTCACTG GATCGTATCTACAACATGCTCCGCATGTTTGTGGTGACTGGGCCTGCACTGGCCGAGATTGACCTGCAGG AGCTGCAGGGCTACCTGCAGAAGAAGGTGCGGGACCAGCAGCTCGTCTACTCGGCCGGCGTCTACCGCCT GCCCAAGAACTGCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207539 protein sequence
Red=Cloning site Green=Tags(s) MAAAVVVAEGDSDSRPGQELLVAWNTVSTGLVPPAALGLVSSRTSGAVPPKEEELRAAVEVLRGHGLHSV LEEWFVEVLQNDLQANISPEFWNAISQCENSADEPQCLLLLLDAFGLLESRLDPYLRSLELLEKWTRLGL LMGTGAQGLREEVHTMLRGVLFFSTPRTFQEMIQRLYGCFLRVYMQSKRKGEGGTDPELEGELDSRYARR RYYRLLQSPLCAGCSSDKQQCWCRQALEQFHQLSQVLHRLSLLERVSAEAVTTTLHQVTRERMEDRCRGE YERSFLREFHKWIERVVGWLGKVFLQDGPARPASPEAGNTLRRWRCHVQRFFYRIYASLRIEELFSIVRD FPDSRPAIEDLKYCLERTDQRQQLLVSLKAALETRLLHPGVNTCDIITLYISAIKALRVLDPSMVILEVA CEPIRRYLRTREDTVRQIVAGLTGDSDGTGDLAVELSKTDPASLETGQDSEDDSGEPEDWVPDPVDADPG KSSSKRRSSDIISLLVSIYGSKDLFINEYRSLLADRLLHQFSFSPEREIRNVELLKLRFGEAPMHFCEVM LKDMADSRRINANIREEDEKRPAEEQPPFGVYAVILSSEFWPPFKDEKLEVPEDIRAALEAYCKKYEQLK AMRTLSWKHTLGLVTMDVELADRTLSVAVTPVQAVILLYFQDQASWTLEELSKAVKMPVALLRRRMSVWL QQGVLREEPPGTFSVIEEERPQDRDNMVLIDSDDESDSGMASQADQKEEELLLFWTYIQAMLTNLESLSL DRIYNMLRMFVVTGPALAEIDLQELQGYLQKKVRDQQLVYSAGVYRLPKNCS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013366 |
| ORF Size | 2466 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013366.4 |
| RefSeq Size | 2733 bp |
| RefSeq ORF | 2469 bp |
| Locus ID | 29882 |
| UniProt ID | Q9UJX6 |
| Domains | CULLIN |
| Protein Families | Druggable Genome |
| Protein Pathways | Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis |
| MW | 93.8 kDa |
| Gene Summary | A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207539L1 | Lenti ORF clone of Human anaphase promoting complex subunit 2 (ANAPC2), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC207539L2 | Lenti ORF clone of Human anaphase promoting complex subunit 2 (ANAPC2), mGFP tagged |
CNY 7,700.00 |
|
| RC207539L3 | Lenti ORF clone of Human anaphase promoting complex subunit 2 (ANAPC2), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC207539L4 | Lenti ORF clone of Human anaphase promoting complex subunit 2 (ANAPC2), mGFP tagged |
CNY 7,700.00 |
|
| RG207539 | ANAPC2 (tGFP-tagged) - Human anaphase promoting complex subunit 2 (ANAPC2) |
CNY 6,370.00 |
|
| SC115268 | ANAPC2 (untagged)-Human anaphase promoting complex subunit 2 (ANAPC2) |
CNY 2,950.00 |
|
| SC324311 | ANAPC2 (untagged)-Human anaphase promoting complex subunit 2 (ANAPC2) |
CNY 2,950.00 |
