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DHDH (NM_014475) Human Tagged ORF Clone
CAT＃: RC207537
DHDH (Myc-DDK-tagged)-Human dihydrodiol dehydrogenase (dimeric) (DHDH)
ORF Plasmid: tGFP
"NM_014475" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2DD; HUM2DD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207537 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGCGCTGGGGCATCGTGTCTGTCGGCCTCATCTCCAGCGACTTCACAGCCGTGCTGCAGACGC TGCCTCGCTCTGAGCACCAGGTGGTGGCGGTGGCGGCCCGCGATCTGAGCCGTGCGAAGGAGTTTGCACA GAAACACGACATCCCCAAGGCCTACGGCTCCTATGAGGAGCTGGCCAAGGACCCGAGCGTGGAGGTGGCC TACATTGGCACCCAGCACCCCCAGCACAAGGCGGCGGTGATGCTGTGCTTGGCGGCGGGCAAGGCCGTTC TGTGCGAGAAGCCCACGGGCGTGAACGCGGCGGAAGTTCGCGAGATGGTCGCGGAGGCCCGATCCCGAGC CCTCTTCCTTATGGAGGCCATCTGGACCCGCTTCTTTCCTGCCTCCGAGGCTCTGAGGTCTGTTTTGGCC CAGGGAACTCTAGGAGACCTCCGGGTGGCTCGGGCAGAATTTGGGAAGAATCTCATCCACGTTCCCCGGG CCGTAGACCGGGCCCAGGCTGGGGGGGCCCTGCTGGACATCGGCATCTACTGTGTCCAGTTCACCTCCAT GGTCTTTGGAGGGCAGAAGCCAGAGAAGATTTCTGTCGTGGGAAGGCGTCATGAAACAGGTGTGGATGAC ACTGTCACGGTGCTCCTGCAGTACCCAGGGGAGGTCCATGGCAGCTTCACCTGCAGCATCACCGTGCAGC TCTCCAACACGGCCTCCGTGAGCGGCACCAAGGGCATGGTACAGCTCCTCAACCCCTGCTGGTGCCCGAC CGAGCTGGTGGTGAAGGGGGAGCATAAGGAGTTCCCGCTGCCCCCAGTCCCAAAGGACTGCAATTTTGAC AACGGGGCAGGCATGAGTTATGAGGCCAAGCACGTCTGGGAGTGCCTACGCAAGGGTATGAAGGAAAGTC CTGTGATTCCCCTGTCGGAAAGTGAGCTCCTGGCTGACATCCTTGAAGAGGTGAGGAAGGCCATTGGAGT CACCTTCCCCCAAGACAAACGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC207537 protein sequence
Red=Cloning site Green=Tags(s) MALRWGIVSVGLISSDFTAVLQTLPRSEHQVVAVAARDLSRAKEFAQKHDIPKAYGSYEELAKDPSVEVA YIGTQHPQHKAAVMLCLAAGKAVLCEKPTGVNAAEVREMVAEARSRALFLMEAIWTRFFPASEALRSVLA QGTLGDLRVARAEFGKNLIHVPRAVDRAQAGGALLDIGIYCVQFTSMVFGGQKPEKISVVGRRHETGVDD TVTVLLQYPGEVHGSFTCSITVQLSNTASVSGTKGMVQLLNPCWCPTELVVKGEHKEFPLPPVPKDCNFD NGAGMSYEAKHVWECLRKGMKESPVIPLSESELLADILEEVRKAIGVTFPQDKR SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014475 |
| ORF Size | 1002 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014475.4 |
| RefSeq Size | 1098 bp |
| RefSeq ORF | 1005 bp |
| Locus ID | 27294 |
| UniProt ID | Q9UQ10 |
| Protein Pathways | Metabolism of xenobiotics by cytochrome P450 |
| MW | 36.4 kDa |
| Gene Summary | This gene encodes an enzyme that belongs to the family of dihydrodiol dehydrogenases, which exist in multiple forms in mammalian tissues and are involved in the metabolism of xenobiotics and sugars. These enzymes catalyze the NADP1-linked oxidation of transdihydrodiols of aromatic hydrocarbons to corresponding catechols. This enzyme is a dimeric dihydrodiol dehydrogenase, and it differs from monomeric dihydrodiol dehydrogenases in its high substrate specificity for trans-dihydrodiols of aromatic hydrocarbons in the oxidative direction. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207537L3 | Lenti ORF clone of Human dihydrodiol dehydrogenase (dimeric) (DHDH), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207537L4 | Lenti ORF clone of Human dihydrodiol dehydrogenase (dimeric) (DHDH), mGFP tagged |
CNY 5,890.00 |
|
| RG207537 | DHDH (tGFP-tagged) - Human dihydrodiol dehydrogenase (dimeric) (DHDH) |
CNY 4,370.00 |
|
| SC321734 | DHDH (untagged)-Human dihydrodiol dehydrogenase (dimeric) (DHDH) |
CNY 3,656.00 |
