B3GALT4 (NM_003782) Human Tagged ORF Clone

CAT#: RC207430

B3GALT4 (Myc-DDK-tagged)-Human UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 4 (B3GALT4)

ORF Plasmid: DDK tGFP



  "NM_003782" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit polyclonal anti-B3GALT4 antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms BETA3GALT4; GALT2; GALT4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC207430 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCTCAGGCTCTTCCGGCGCCTCCTTCTCGCCGCTTTGCTGCTGGTGATCGTCTGGACCCTCTTCG
GGCCTTCGGGGTTGGGGGAGGAGCTGCTGAGCCTCTCACTAGCCTCCCTGCTCCCAGCCCCCGCCTCACC
GGGGCCGCCCCTGGCCCTGCCCCGCCTCTTGATCCCCAACCAGGAAGCTTGCAGTGGTCCCGGGGCCCCT
CCCTTCCTGCTCATCCTGGTGTGCACGGCTCCGGAGAACCTGAACCAGAGAAACGCCATTCGGGCTTCGT
GGGGCGGGCTGCGCGAGGCCCGGGGGCTCAGGGTACAGACGCTATTCTTGCTGGGAGAGCCGAACGCACA
GCACCCCGTGTGGGGTTCCCAGGGGAGTGACCTGGCCTCGGAGTCAGCAGCCCAGGGGGATATCTTGCAG
GCCGCCTTCCAGGACTCCTACCGCAACCTCACCCTAAAGACCCTCAGCGGGCTGAACTGGGCTGAGAAAC
ACTGCCCCATGGCCCGATACGTCCTCAAGACGGACGATGATGTGTATGTCAACGTCCCTGAACTGGTATC
AGAGCTGGTCTTGCGAGGGGGCCGTTGGGGGCAATGGGAGAGAAGCACGGAACCCCAGAGAGAGGCTGAG
CAGGAAGGAGGCCAGGTTTTGCACAGCGAGGAAGTGCCTCTTCTGTACTTGGGCCGGGTGCACTGGCGCG
TGAACCCCTCTCGGACACCGGGGGGCAGGCACCGCGTATCAGAGGAGCAGTGGCCTCACACCTGGGGCCC
CTTTCCACCCTATGCCTCAGGCACGGGGTATGTGCTGTCAGCGTCTGCTGTGCAGCTCATTCTCAAGGTG
GCCAGCCGGGCACCCCTTCTCCCATTAGAGGATGTCTTTGTGGGGGTAAGTGCCCGACGAGGAGGCCTCG
CCCCAACACAGTGTGTCAAGCTGGCTGGTGCCACCCACTACCCGCTAGACCGGTGCTGCTATGGGAAATT
CCTGCTGACGTCCCACAGGCTGGACCCCTGGAAGATGCAGGAAGCCTGGAAGCTGGTGGGTGGCTCTGAC
GGGGAAAGGACTGCGCCCTTTTGCTCCTGGTTCCAGGGAGTCCTGGGCATCCTGCGGTGTCGAGCAATAG
CCTGGCTTCAGAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC207430 protein sequence
Red=Cloning site Green=Tags(s)

MQLRLFRRLLLAALLLVIVWTLFGPSGLGEELLSLSLASLLPAPASPGPPLALPRLLIPNQEACSGPGAP
PFLLILVCTAPENLNQRNAIRASWGGLREARGLRVQTLFLLGEPNAQHPVWGSQGSDLASESAAQGDILQ
AAFQDSYRNLTLKTLSGLNWAEKHCPMARYVLKTDDDVYVNVPELVSELVLRGGRWGQWERSTEPQREAE
QEGGQVLHSEEVPLLYLGRVHWRVNPSRTPGGRHRVSEEQWPHTWGPFPPYASGTGYVLSASAVQLILKV
ASRAPLLPLEDVFVGVSARRGGLAPTQCVKLAGATHYPLDRCCYGKFLLTSHRLDPWKMQEAWKLVGGSD
GERTAPFCSWFQGVLGILRCRAIAWLQS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003782
ORF Size 1134 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003782.4
RefSeq Size 1704 bp
RefSeq ORF 1137 bp
Locus ID 8705
UniProt ID O96024
Protein Families Transmembrane
Protein Pathways Glycosphingolipid biosynthesis - ganglio series, Metabolic pathways
MW 41.5 kDa
Gene Summary This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). This gene is oriented telomere to centromere in close proximity to the ribosomal protein S18 gene. The functionality of the encoded protein is limited to ganglioseries glycolipid biosynthesis. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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