SFRP1 (NM_003012) Human Tagged ORF Clone
CAT#: RC207328
SFRP1 (Myc-DDK-tagged)-Human secreted frizzled-related protein 1 (SFRP1)
ORF Plasmid: tGFP
"NM_003012" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Cited in 4 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | FRP; FRP-1; FRP1; FrzA; SARP2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC207328 representing NM_003012
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCATCGGGCGCAGCGAGGGGGGCCGCCGCGGGGCAGCCCTGGGCGTGCTGCTGGCGCTGGGCGCGG CGCTTCTGGCCGTGGGCTCGGCCAGCGAGTACGACTACGTGAGCTTCCAGTCGGACATCGGCCCGTACCA GAGCGGGCGCTTCTACACCAAGCCACCTCAGTGCGTGGACATCCCCGCGGACCTGCGGCTGTGCCACAAC GTGGGCTACAAGAAGATGGTGCTGCCCAACCTGCTGGAGCACGAGACCATGGCGGAGGTGAAGCAGCAGG CCAGCAGCTGGGTGCCCCTGCTCAACAAGAACTGCCACGCCGGCACCCAGGTCTTCCTCTGCTCGCTCTT CGCGCCCGTCTGCCTGGACCGGCCCATCTACCCGTGTCGCTGGCTCTGCGAGGCCGTGCGCGACTCGTGC GAGCCGGTCATGCAGTTCTTCGGCTTCTACTGGCCCGAGATGCTTAAGTGTGACAAGTTCCCCGAGGGGG ACGTCTGCATCGCCATGACGCCGCCCAATGCCACCGAAGCCTCCAAGCCCCAAGGCACAACGGTGTGTCC TCCCTGTGACAACGAGTTGAAATCTGAGGCCATCATTGAACATCTCTGTGCCAGCGAGTTTGCACTGAGG ATGAAAATAAAAGAAGTGAAAAAAGAAAATGGCGACAAGAAGATTGTCCCCAAGAAGAAGAAGCCCCTGA AGTTGGGGCCCATCAAGAAGAAGGACCTGAAGAAGCTTGTGCTGTACCTGAAGAATGGGGCTGACTGTCC CTGCCACCAGCTGGACAACCTCAGCCACCACTTCCTCATCATGGGCCGCAAGGTGAAGAGCCAGTACTTG CTGACGGCCATCCACAAGTGGGACAAGAAAAACAAGGAGTTCAAAAACTTCATGAAGAAAATGAAAAACC ATGAGTGCCCCACCTTTCAGTCCGTGTTTAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207328 representing NM_003012
Red=Cloning site Green=Tags(s) MGIGRSEGGRRGAALGVLLALGAALLAVGSASEYDYVSFQSDIGPYQSGRFYTKPPQCVDIPADLRLCHN VGYKKMVLPNLLEHETMAEVKQQASSWVPLLNKNCHAGTQVFLCSLFAPVCLDRPIYPCRWLCEAVRDSC EPVMQFFGFYWPEMLKCDKFPEGDVCIAMTPPNATEASKPQGTTVCPPCDNELKSEAIIEHLCASEFALR MKIKEVKKENGDKKIVPKKKKPLKLGPIKKKDLKKLVLYLKNGADCPCHQLDNLSHHFLIMGRKVKSQYL LTAIHKWDKKNKEFKNFMKKMKNHECPTFQSVFK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_003012 |
ORF Size | 942 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003012.5 |
RefSeq Size | 4465 bp |
RefSeq ORF | 945 bp |
Locus ID | 6422 |
UniProt ID | Q8N474 |
Domains | FRI, NTR |
Protein Families | Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway, Transmembrane |
Protein Pathways | Wnt signaling pathway |
MW | 35.2 kDa |
Gene Summary | This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina. [provided by RefSeq, Sep 2009] |
Citations (4)
The use of this cDNA Clones has been cited in the following citations: |
---|
sFRP1 exerts effects on gastric cancer cells through GSK3β/Rac1‑mediated restraint of TGFβ/Smad3 signaling
,Peng, J;Liang, S;Li, L;,
Oncol Rep
,PubMed ID 30542739
[SFRP1]
|
Restoration of secreted frizzled-related protein 1 suppresses growth and increases cisplatin sensitivity in laryngeal carcinoma cells by downregulating NHE1
,Wu, KM;Li, ZQ;Yi, WZ;Wu, MH;Jiang, MJ;Zhang, Y;Zheng, HL;Chen, W;,
Int J Clin Exp Patho
[SFRP1]
|
Secreted frizzled-related protein 1 overexpression in gastric cancer: Relationship with radiological findings of dual-energy spectral CT and PET-CT
,Lin, H;Yang, G;Ding, B;Zhang, M;Zhang, M;Yan, F;Qu, Y;Zhang, H;,
Sci Rep
,PubMed ID 28169332
[SFRP1]
|
Functional analyses and prognostic significance of SFRP1 expression in bladder cancer
,Rogler, A;Kendziorra, E;Giedl, J;Stoehr, C;Taubert, H;Goebell, PJ;Wullich, B;Stöckle, M;Lehmann, J;Petsch, S;Hartmann, A;Stoehr, R;,
J. Cancer Res. Clin. Oncol.
,PubMed ID 25732201
[SFRP1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207328L1 | Lenti ORF clone of Human secreted frizzled-related protein 1 (SFRP1), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC207328L2 | Lenti ORF clone of Human secreted frizzled-related protein 1 (SFRP1), mGFP tagged |
CNY 4,800.00 |
|
RC207328L3 | Lenti ORF clone of Human secreted frizzled-related protein 1 (SFRP1), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC207328L4 | Lenti ORF clone of Human secreted frizzled-related protein 1 (SFRP1), mGFP tagged |
CNY 4,800.00 |
|
RG207328 | SFRP1 (tGFP-tagged) - Human secreted frizzled-related protein 1 (SFRP1) |
CNY 4,000.00 |
|
SC118246 | SFRP1 (untagged)-Human secreted frizzled-related protein 1 (SFRP1) |
CNY 2,400.00 |