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GDF 5 (GDF5) (NM_000557) Human Tagged ORF Clone
CAT＃: RC207105
GDF5 (Myc-DDK-tagged)-Human growth differentiation factor 5 (GDF5)
ORF Plasmid: tGFP
"NM_000557" in other vectors (6)
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CNY 3,728.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BDA1C; BMP-14; BMP14; CDMP1; DUPANS; LAP-4; LAP4; OS5; SYM1B; SYNS2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207105 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGACTCCCCAAACTCCTCACTTTCTTGCTTTGGTACCTGGCTTGGCTGGACCTGGAATTCATCTGCA CTGTGTTGGGTGCCCCTGACTTGGGCCAGAGACCCCAGGGGACCAGGCCAGGATTGGCCAAAGCAGAGGC CAAGGAGAGGCCCCCCCTGGCCCGGAACGTCTTCAGGCCAGGGGGTCACAGCTATGGTGGGGGGGCCACC AATGCCAATGCCAGGGCAAAGGGAGGCACCGGGCAGACAGGAGGCCTGACACAGCCCAAGAAGGATGAAC CCAAAAAGCTGCCCCCCAGACCGGGCGGCCCTGAACCCAAGCCAGGACACCCTCCCCAAACAAGGCAGGC TACAGCCCGGACTGTGACCCCAAAAGGACAGCTTCCCGGAGGCAAGGCACCCCCAAAAGCAGGATCTGTC CCCAGCTCCTTCCTGCTGAAGAAGGCCAGGGAGCCCGGGCCCCCACGAGAGCCCAAGGAGCCGTTTCGCC CACCCCCCATCACACCCCACGAGTACATGCTCTCGCTGTACAGGACGCTGTCCGATGCTGACAGAAAGGG AGGCAACAGCAGCGTGAAGTTGGAGGCTGGCCTGGCCAACACCATCACCAGCTTTATTGACAAAGGGCAA GATGACCGAGGTCCCGTGGTCAGGAAGCAGAGGTACGTGTTTGACATTAGTGCCCTGGAGAAGGATGGGC TGCTGGGGGCCGAGCTGCGGATCTTGCGGAAGAAGCCCTCGGACACGGCCAAGCCAGCGGCCCCCGGAGG CGGGCGGGCTGCCCAGCTGAAGCTGTCCAGCTGCCCCAGCGGCCGGCAGCCGGCCTCCTTGCTGGATGTG CGCTCCGTGCCAGGCCTGGACGGATCTGGCTGGGAGGTGTTCGACATCTGGAAGCTCTTCCGAAACTTTA AGAACTCGGCCCAGCTGTGCCTGGAGCTGGAGGCCTGGGAACGGGGCAGGGCCGTGGACCTCCGTGGCCT GGGCTTCGACCGCGCCGCCCGGCAGGTCCACGAGAAGGCCCTGTTCCTGGTGTTTGGCCGCACCAAGAAA CGGGACCTGTTCTTTAATGAGATTAAGGCCCGCTCTGGCCAGGACGATAAGACCGTGTATGAGTACCTGT TCAGCCAGCGGCGAAAACGGCGGGCCCCACTGGCCACTCGCCAGGGCAAGCGACCCAGCAAGAACCTTAA GGCTCGCTGCAGTCGGAAGGCACTGCATGTCAACTTCAAGGACATGGGCTGGGACGACTGGATCATCGCA CCCCTTGAGTACGAGGCTTTCCACTGCGAGGGGCTGTGCGAGTTCCCATTGCGCTCCCACCTGGAGCCCA CGAATCATGCAGTCATCCAGACCCTGATGAACTCCATGGACCCCGAGTCCACACCACCCACCTGCTGTGT TCCCACGCGGCTGAGTCCCATCAGCATCCTCTTCATTGACTCTGCCAACAACGTGGTGTATAAGCAGTAT GAGGACATGGTCGTGGAGTCGTGTGGCTGCAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207105 protein sequence
Red=Cloning site Green=Tags(s) MRLPKLLTFLLWYLAWLDLEFICTVLGAPDLGQRPQGTRPGLAKAEAKERPPLARNVFRPGGHSYGGGAT NANARAKGGTGQTGGLTQPKKDEPKKLPPRPGGPEPKPGHPPQTRQATARTVTPKGQLPGGKAPPKAGSV PSSFLLKKAREPGPPREPKEPFRPPPITPHEYMLSLYRTLSDADRKGGNSSVKLEAGLANTITSFIDKGQ DDRGPVVRKQRYVFDISALEKDGLLGAELRILRKKPSDTAKPAAPGGGRAAQLKLSSCPSGRQPASLLDV RSVPGLDGSGWEVFDIWKLFRNFKNSAQLCLELEAWERGRAVDLRGLGFDRAARQVHEKALFLVFGRTKK RDLFFNEIKARSGQDDKTVYEYLFSQRRKRRAPLATRQGKRPSKNLKARCSRKALHVNFKDMGWDDWIIA PLEYEAFHCEGLCEFPLRSHLEPTNHAVIQTLMNSMDPESTPPTCCVPTRLSPISILFIDSANNVVYKQY EDMVVESCGCR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000557 |
| ORF Size | 1503 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000557.5 |
| RefSeq Size | 2344 bp |
| RefSeq ORF | 1506 bp |
| Locus ID | 8200 |
| UniProt ID | P43026 |
| Protein Families | Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - TGFb/BMP signaling pathway |
| Protein Pathways | Cytokine-cytokine receptor interaction, TGF-beta signaling pathway |
| MW | 55.4 kDa |
| Gene Summary | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates the development of numerous tissue and cell types, including cartilage, joints, brown fat, teeth, and the growth of neuronal axons and dendrites. Mutations in this gene are associated with acromesomelic dysplasia, brachydactyly, chondrodysplasia, multiple synostoses syndrome, proximal symphalangism, and susceptibility to osteoarthritis. [provided by RefSeq, Aug 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207105L1 | Lenti ORF clone of Human growth differentiation factor 5 (GDF5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207105L2 | Lenti ORF clone of Human growth differentiation factor 5 (GDF5), mGFP tagged |
CNY 5,890.00 |
|
| RC207105L3 | Lenti ORF clone of Human growth differentiation factor 5 (GDF5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207105L4 | Lenti ORF clone of Human growth differentiation factor 5 (GDF5), mGFP tagged |
CNY 5,890.00 |
|
| RG207105 | GDF5 (tGFP-tagged) - Human growth differentiation factor 5 (GDF5) |
CNY 4,370.00 |
|
| SC125956 | GDF5 (untagged)-Human growth differentiation factor 5 (GDF5) |
CNY 2,950.00 |
