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MAGED1 (NM_001005332) Human Tagged ORF Clone
CAT＃: RC207077
MAGED1 (Myc-DDK-tagged)-Human melanoma antigen family D, 1 (MAGED1), transcript variant 3
ORF Plasmid: tGFP
"NM_001005332" in other vectors (4)
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CNY 5,696.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DLXIN-1; NRAGE |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207077 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGAAAATGGACTGTGGTGCGGGCCTCCTCGGCTTCCAGGCTGAGGCCTCCGTAGAAGACAGCG CCTTGCTTATGCAGACCTTGATGGAGGCCATCCAGATCTCAGAGGCTCCACCTACTAACCAGGCCACCGC AGCTGCTAGTCCCCAGAGTTCACAGCCCCCAACTGCCAATGAGATGGCTGACATTCAGGTTTCAGCAGCT GCCGCTAGGCCTAAGTCAGCCTTTAAAGTCCAGAATGCCACCACAAAAGGCCCAAATGGTGTCTATGATT TCTCTCAGGCTCATAATGCCAAGGATGTGCCCAACACGCAGCCCAAGGCAGCCTTTAAGTCCCAAAATGC TACCCCAAAGGGTCCAAATGCTGCCTATGATTTTTCCCAGGCAGCAACCACTGGTGAGTTAGCTGCTAAC AAGTCTGAGATGGCCTTCAAGGCCCAGAATGCCACTACTAAAGTGGGCCCAAATGCCACCTACAATTTCT CTCAGTCTCTCAATGCCAATGACCTGGCCAACAGCAGGCCTAAGACCCCTTTCAAGGCTTGGAATGATAC CACTAAGGCCCCAACAGCTGATACCCAGACCCAGAATGTAAATCAGGCCAAAATGGCCACTTCCCAGGCT GACATAGAGACCGACCCAGGTATCTCTGAACCTGACGGTGCAACTGCACAGACATCAGCAGATGGTTCCC AGGCTCAGAATCTGGAGTCCCGGACAATAATTCGGGGCAAGAGGACCCGCAAGATTAATAACTTGAATGT TGAAGAGAACAGCAGTGGGGATCAGAGGCGGGCCCCACTGGCTGCAGGGACCTGGAGGTCTGCACCAGTT CCAGTGACCACTCAGAACCCACCTGGCGCACCCCCCAATGTGCTCTGGCAGACGCCATTGGCTTGGCAGA ACCCCTCAGGCTGGCAAAACCAGACAGCCAGGCAGACCCCACCAGCACGTCAGAGCCCTCCAGCTAGGCA GACCCCACCAGCCTGGCAGAACCCAGTCGCTTGGCAGAACCCAGTGATTTGGCCAAACCCAGTAATCTGG CAGAACCCAGTGATCTGGCCAAACCCCATTGTCTGGCCCGGCCCTGTTGTCTGGCCGAATCCACTGGCCT GGCAGAATCCACCTGGATGGCAGACTCCACCTGGATGGCAGACCCCACCGGGCTGGCAGGGTCCTCCAGA CTGGCAAGGTCCTCCTGACTGGCCGCTACCACCCGACTGGCCACTGCCACCTGATTGGCCACTTCCCACT GACTGGCCACTACCACCTGACTGGATCCCCGCTGATTGGCCAATTCCACCTGACTGGCAGAACCTGCGCC CCTCGCCTAACCTGCGCCCTTCTCCCAACTCGCGTGCCTCACAGAACCCAGGTGCTGCACAGCCCCGAGA TGTGGCCCTTCTTCAGGAAAGAGCAAATAAGTTGGTCAAGTACTTGATGCTTAAGGACTACACAAAGGTG CCCATCAAGCGCTCAGAAATGCTGAGAGATATCATCCGTGAATACACTGATGTTTATCCAGAAATCATTG AACGTGCATGCTTTGTCCTAGAGAAGAAATTTGGGATTCAACTGAAAGAAATTGACAAAGAAGAACACCT GTATATTCTCATCAGTACCCCCGAGTCCCTGGCTGGCATACTGGGAACGACCAAAGACACACCCAAGCTC GGTCTCCTCTTGGTGATTCTGGGTGTCATCTTCATGAATGGCAACCGTGCCAGTGAGGCTGTCCTCTGGG AGGCACTACGCAAGATGGGACTGCGTCCTGGGGTGAGACATCCCCTCCTTGGAGATCTAAGGAAACTTCT CACCTATGAGTTTGTAAAGCAGAAATACCTGGACTACAGACGAGTGCCCAACAGCAACCCCCCGGAGTAT GAGTTCCTCTGGGGCCTCCGTTCCTACCATGAGACTAGCAAGATGAAAGTGCTGAGATTCATTGCAGAGG TTCAGAAAAGAGACCCTCGTGACTGGACTGCACAGTTCATGGAGGCTGCAGATGAGGCCTTGGATGCTCT GGATGCTGCTGCAGCTGAGGCCGAAGCCCGGGCTGAAGCAAGAACCCGCATGGGAATTGGAGATGAGGCT GTGTCTGGGCCCTGGAGCTGGGATGACATTGAGTTTGAGCTGCTGACCTGGGATGAGGAAGGAGATTTTG GAGATCCCTGGTCCAGAATTCCATTTACCTTCTGGGCCAGATACCACCAGAATGCCCGCTCCAGATTCCC TCAGACCTTTGCCGGTCCCATTATTGGTCCTGGTGGTACAGCCAGTGCCAACTTCGCTGCCAACTTTGGT GCCATTGGTTTCTTCTGGGTTGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207077 protein sequence
Red=Cloning site Green=Tags(s) MAQKMDCGAGLLGFQAEASVEDSALLMQTLMEAIQISEAPPTNQATAAASPQSSQPPTANEMADIQVSAA AARPKSAFKVQNATTKGPNGVYDFSQAHNAKDVPNTQPKAAFKSQNATPKGPNAAYDFSQAATTGELAAN KSEMAFKAQNATTKVGPNATYNFSQSLNANDLANSRPKTPFKAWNDTTKAPTADTQTQNVNQAKMATSQA DIETDPGISEPDGATAQTSADGSQAQNLESRTIIRGKRTRKINNLNVEENSSGDQRRAPLAAGTWRSAPV PVTTQNPPGAPPNVLWQTPLAWQNPSGWQNQTARQTPPARQSPPARQTPPAWQNPVAWQNPVIWPNPVIW QNPVIWPNPIVWPGPVVWPNPLAWQNPPGWQTPPGWQTPPGWQGPPDWQGPPDWPLPPDWPLPPDWPLPT DWPLPPDWIPADWPIPPDWQNLRPSPNLRPSPNSRASQNPGAAQPRDVALLQERANKLVKYLMLKDYTKV PIKRSEMLRDIIREYTDVYPEIIERACFVLEKKFGIQLKEIDKEEHLYILISTPESLAGILGTTKDTPKL GLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPLLGDLRKLLTYEFVKQKYLDYRRVPNSNPPEY EFLWGLRSYHETSKMKVLRFIAEVQKRDPRDWTAQFMEAADEALDALDAAAAEAEARAEARTRMGIGDEA VSGPWSWDDIEFELLTWDEEGDFGDPWSRIPFTFWARYHQNARSRFPQTFAGPIIGPGGTASANFAANFG AIGFFWVE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001005332 |
| ORF Size | 2334 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001005332.2 |
| RefSeq Size | 2665 bp |
| RefSeq ORF | 2337 bp |
| Locus ID | 9500 |
| UniProt ID | Q9Y5V3 |
| Protein Families | Druggable Genome |
| Protein Pathways | Neurotrophin signaling pathway |
| MW | 86.2 kDa |
| Gene Summary | This gene is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207077L3 | Lenti ORF clone of Human melanoma antigen family D, 1 (MAGED1), transcript variant 3, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC207077L4 | Lenti ORF clone of Human melanoma antigen family D, 1 (MAGED1), transcript variant 3, mGFP tagged |
CNY 7,700.00 |
|
| RG207077 | MAGED1 (tGFP-tagged) - Human melanoma antigen family D, 1 (MAGED1), transcript variant 3 |
CNY 6,370.00 |
|
| SC300905 | MAGED1 (untagged)-Human melanoma antigen family D, 1 (MAGED1), transcript variant 3 |
CNY 7,220.00 |
