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BUB1 (NM_004336) Human Tagged ORF Clone
CAT＃: RC207013
BUB1 (Myc-DDK-tagged)-Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1)
ORF Plasmid: tGFP
"NM_004336" in other vectors (7)
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CNY 7,552.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BUB1A; BUB1L; hBUB1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207013 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACACCCCGGAAAATGTCCTTCAGATGCTTGAAGCCCACATGCAGAGCTACAAGGGCAATGACCCTC TTGGTGAATGGGAAAGATACATACAGTGGGTAGAAGAGAATTTTCCTGAGAATAAAGAATACTTGATAAC TTTACTAGAACATTTAATGAAGGAATTTTTAGATAAGAAGAAATACCACAATGACCCAAGATTCATCAGT TATTGTTTAAAATTTGCTGAGTACAACAGTGACCTCCATCAATTTTTTGAGTTTCTGTACAACCATGGGA TTGGAACCCTGTCATCCCCTCTGTACATTGCCTGGGCGGGGCATCTGGAAGCCCAAGGAGAGCTGCAGCA TGCCAGTGCTGTCCTTCAGAGAGGAATTCAAAACCAGGCTGAACCCAGAGAGTTCCTGCAACAACAATAC AGGTTATTTCAGACACGCCTCACTGAAACCCATTTGCCAGCTCAAGCTAGAACCTCAGAACCTCTGCATA ATGTTCAGGTTTTAAATCAAATGATAACATCAAAATCAAATCCAGGAAATAACATGGCCTGCATTTCTAA GAATCAGGGTTCAGAGCTTTCTGGAGTGATATCTTCAGCTTGTGATAAAGAGTCAAATATGGAACGAAGA GTGATCACGATTTCTAAATCAGAATATTCTGTGCACTCATCTTTGGCATCCAAAGTTGATGTTGAGCAGG TTGTTATGTATTGCAAGGAGAAGCTTATTCGTGGGGAATCAGAATTTTCCTTTGAAGAATTGAGAGCCCA GAAATACAATCAACGGAGAAAGCATGAGCAATGGGTAAATGAAGACAGACATTATATGAAAAGGAAAGAA GCAAATGCTTTTGAAGAACAGCTATTAAAACAGAAAATGGATGAACTTCATAAGAAGTTGCATCAGGTGG TGGAGACATCCCATGAGGATCTGCCCGCTTCCCAGGAAAGGTCCGAGGTTAATCCAGCACGTATGGGGCC AAGTGTAGGCTCCCAGCAGGAACTGAGAGCGCCATGTCTTCCAGTAACCTATCAGCAGACACCAGTGAAC ATGGAAAAGAACCCAAGAGAGGCACCTCCTGTTGTTCCTCCTTTGGCAAATGCTATTTCTGCAGCTTTGG TGTCCCCAGCCACCAGCCAGAGCATTGCTCCTCCTGTTCCTTTGAAAGCCCAGACAGTAACAGACTCCAT GTTTGCAGTGGCCAGCAAAGATGCTGGATGTGTGAATAAGAGTACTCATGAATTCAAGCCACAGAGTGGA GCAGAGATCAAAGAAGGGTGTGAAACACATAAGGTTGCCAACACAAGTTCTTTTCACACAACTCCAAACA CATCACTGGGAATGGTTCAGGCAACGCCATCCAAAGTGCAGCCATCACCCACCGTGCACACAAAAGAAGC ATTAGGTTTCATCATGAATATGTTTCAGGCTCCTACACTTCCTGATATTTCTGATGACAAAGATGAATGG CAATCTCTAGATCAAAATGAAGATGCATTTGAAGCCCAGTTTCAAAAAAATGTAAGGTCATCTGGGGCTT GGGGAGTCAATAAGATCATCTCTTCTTTGTCATCTGCTTTTCATGTGTTTGAAGATGGAAACAAAGAAAA TTATGGATTACCACAGCCTAAAAATAAACCCACAGGAGCCAGGACCTTTGGAGAACGCTCTGTCAGCAGA CTTCCTTCAAAACCAAAGGAGGAAGTGCCTCATGCTGAAGAGTTTTTGGATGACTCAACTGTATGGGGTA TTCGCTGCAACAAAACCCTGGCACCCAGTCCTAAGAGCCCAGGAGACTTCACATCTGCTGCACAACTTGC GTCTACACCATTCCACAAGCTTCCAGTGGAGTCAGTGCACATTTTAGAAGATAAAGAAAATGTGGTAGCA AAACAGTGTACCCAGGCGACTTTGGATTCTTGTGAGGAAAACATGGTGGTGCCTTCAAGGGATGGAAAAT TCAGTCCAATTCAAGAGAAAAGCCCAAAACAGGCCTTGTCGTCTCACATGTATTCAGCATCCTTACTTCG TCTGAGCCAGCCTGCTGCAGGTGGGGTACTTACCTGTGAGGCAGAGTTGGGCGTTGAGGCTTGCAGACTC ACAGACACTGACGCTGCCATTGCAGAAGATCCACCAGATGCTATTGCTGGGCTCCAAGCAGAATGGATGC AGATGAGTTCACTTGGGACTGTTGATGCTCCAAACTTCATTGTTGGGAACCCATGGGATGATAAGCTGAT TTTCAAACTTTTATCTGGGCTTTCTAAACCAGTGAGTTCCTATCCAAATACTTTTGAATGGCAATGTAAA CTTCCAGCCATCAAGCCCAAGACTGAATTTCAATTGGGTTCTAAGCTGGTCTATGTCCATCACCTTCTTG GAGAAGGAGCCTTTGCCCAGGTGTACGAAGCTACCCAGGGAGATCTGAATGATGCTAAAAATAAACAGAA ATTTGTTTTAAAGGTCCAAAAGCCTGCCAACCCCTGGGAATTCTACATTGGGACCCAGTTGATGGAAAGA CTAAAGCCATCTATGCAGCACATGTTTATGAAGTTCTATTCTGCCCACTTATTCCAGAATGGCAGTGTAT TAGTAGGAGAGCTCTACAGCTATGGAACATTATTAAATGCCATTAACCTCTATAAAAATACCCCTGAAAA AGTGATGCCTCAAGGTCTTGTCATCTCTTTTGCTATGAGAATGCTTTACATGATTGAGCAAGTGCATGAC TGTGAAATCATTCATGGAGACATTAAACCAGACAATTTCATACTTGGAAACGGATTTTTGGAACAGGATG ATGAAGATGATTTATCTGCTGGCTTGGCACTGATTGACCTGGGTCAGAGTATAGATATGAAACTTTTTCC AAAAGGAACTATATTCACAGCAAAGTGTGAAACATCTGGTTTTCAGTGTGTTGAGATGCTCAGCAACAAA CCATGGAACTACCAGATCGATTACTTTGGGGTTGCTGCAACAGTATATTGCATGCTCTTTGGCACTTACA TGAAAGTGAAAAATGAAGGAGGAGAGTGTAAGCCTGAAGGTCTTTTTAGAAGGCTTCCTCATTTGGATAT GTGGAATGAATTTTTTCATGTTATGTTGAATATTCCAGATTGTCATCATCTTCCATCTTTGGATTTGTTA AGGCAAAAGCTGAAGAAAGTATTTCAACAACACTATACTAACAAGATTAGGGCCCTACGTAATAGGCTAA TTGTACTGCTCTTAGAATGTAAGCGTTCACGAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207013 protein sequence
Red=Cloning site Green=Tags(s) MDTPENVLQMLEAHMQSYKGNDPLGEWERYIQWVEENFPENKEYLITLLEHLMKEFLDKKKYHNDPRFIS YCLKFAEYNSDLHQFFEFLYNHGIGTLSSPLYIAWAGHLEAQGELQHASAVLQRGIQNQAEPREFLQQQY RLFQTRLTETHLPAQARTSEPLHNVQVLNQMITSKSNPGNNMACISKNQGSELSGVISSACDKESNMERR VITISKSEYSVHSSLASKVDVEQVVMYCKEKLIRGESEFSFEELRAQKYNQRRKHEQWVNEDRHYMKRKE ANAFEEQLLKQKMDELHKKLHQVVETSHEDLPASQERSEVNPARMGPSVGSQQELRAPCLPVTYQQTPVN MEKNPREAPPVVPPLANAISAALVSPATSQSIAPPVPLKAQTVTDSMFAVASKDAGCVNKSTHEFKPQSG AEIKEGCETHKVANTSSFHTTPNTSLGMVQATPSKVQPSPTVHTKEALGFIMNMFQAPTLPDISDDKDEW QSLDQNEDAFEAQFQKNVRSSGAWGVNKIISSLSSAFHVFEDGNKENYGLPQPKNKPTGARTFGERSVSR LPSKPKEEVPHAEEFLDDSTVWGIRCNKTLAPSPKSPGDFTSAAQLASTPFHKLPVESVHILEDKENVVA KQCTQATLDSCEENMVVPSRDGKFSPIQEKSPKQALSSHMYSASLLRLSQPAAGGVLTCEAELGVEACRL TDTDAAIAEDPPDAIAGLQAEWMQMSSLGTVDAPNFIVGNPWDDKLIFKLLSGLSKPVSSYPNTFEWQCK LPAIKPKTEFQLGSKLVYVHHLLGEGAFAQVYEATQGDLNDAKNKQKFVLKVQKPANPWEFYIGTQLMER LKPSMQHMFMKFYSAHLFQNGSVLVGELYSYGTLLNAINLYKNTPEKVMPQGLVISFAMRMLYMIEQVHD CEIIHGDIKPDNFILGNGFLEQDDEDDLSAGLALIDLGQSIDMKLFPKGTIFTAKCETSGFQCVEMLSNK PWNYQIDYFGVAATVYCMLFGTYMKVKNEGGECKPEGLFRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLL RQKLKKVFQQHYTNKIRALRNRLIVLLLECKRSRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004336 |
| ORF Size | 3255 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004336.5 |
| RefSeq Size | 3636 bp |
| RefSeq ORF | 3258 bp |
| Locus ID | 699 |
| UniProt ID | O43683 |
| Domains | pkinase, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation |
| MW | 122.4 kDa |
| Gene Summary | This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207013L1 | Lenti ORF clone of Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1), Myc-DDK-tagged |
CNY 9,952.00 |
|
| RC207013L2 | Lenti ORF clone of Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1), mGFP tagged |
CNY 9,952.00 |
|
| RC207013L3 | Lenti ORF clone of Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1), Myc-DDK-tagged |
CNY 10,260.00 |
|
| RC207013L4 | Lenti ORF clone of Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1), mGFP tagged |
CNY 10,260.00 |
|
| RG207013 | BUB1 (tGFP-tagged) - Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1) |
CNY 9,152.00 |
|
| SC110841 | BUB1 (untagged)-Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1) |
CNY 7,560.00 |
|
| SC323526 | BUB1 (untagged)-Kinase deficient mutant (K821M) of Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1) |
CNY 7,560.00 |
