C7orf16 (PPP1R17) (NM_006658) Human Tagged ORF Clone

CAT#: RC207007

PPP1R17 (Myc-DDK-tagged)-Human chromosome 7 open reading frame 16 (C7orf16), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_006658" in other vectors (4)

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CNY 1,200.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


PPP1R17 mouse monoclonal antibody,clone OTI2E5
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms C7orf16; GSBS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC207007 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGTCCACTGAGCAAATGCAGCCACTGGAAGTCTCAGAAGACAGACTGGACAAGCTAGACCCTCGTT
GCAGCCACTTAGATGATCTTTCAGACCAGTTCATTAAGGACTGTGATCTCAAAAAGAAGCCTAGAAAGGG
AAAAAATGTACAGGCCACCCTGAATGTTGAGTCAGACCAAAAAAAACCAAGGAGGAAAGATACACCGGCG
CTGCACATCCCACCTTTCATACCAGGTGTGTTTTCAGAACATTTAATTAAAAGATACGATGTTCAAGAGA
GACATCCAAAGGGCAAAATGATCCCTGTTCTTCATAACACTGACCTGGAACAGAAAAAGCCAAGGAGAAA
AGACACACCTGCCCTGCACATGTCCCCCTTTGCAGCAGGTGTGACATTGCTCAGGGATGAGAGACCCAAA
GCAATCGTGGAAGATGACGAAAAGGATGGTGACAAGATAGCTATT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC207007 protein sequence
Red=Cloning site Green=Tags(s)

MMSTEQMQPLEVSEDRLDKLDPRCSHLDDLSDQFIKDCDLKKKPRKGKNVQATLNVESDQKKPRRKDTPA
LHIPPFIPGVFSEHLIKRYDVQERHPKGKMIPVLHNTDLEQKKPRRKDTPALHMSPFAAGVTLLRDERPK
AIVEDDEKDGDKIAI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006658
ORF Size 465 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006658.2, NP_006649.1
RefSeq Size 1966 bp
RefSeq ORF 468 bp
Locus ID 10842
UniProt ID O96001
Protein Pathways Long-term depression
MW 17.9 kDa
Gene Summary The protein encoded by this gene is found primarily in cerebellar Purkinje cells, where it functions as a protein phosphatase inhibitor. The encoded protein is a substrate for cGMP-dependent protein kinase. An allele of this gene was discovered that increases susceptibility to hypercholesterolemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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