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B3GNT5 (NM_032047) Human Tagged ORF Clone
CAT＃: RC206965
B3GNT5 (Myc-DDK-tagged)-Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5)
ORF Plasmid: tGFP
"NM_032047" in other vectors (7)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | B3GN-T5; beta3Gn-T5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206965 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAATGTTGGTTAGTGGCAGAAGAGTCAAAAAATGGCAGTTAATTATTCAGTTATTTGCTACTTGTT TTTTAGCGAGCCTCATGTTTTTTTGGGAACCAATCGATAATCACATTGTGAGCCATATGAAGTCATATTC TTACAGATACCTCATAAATAGCTATGACTTTGTGAATGATACCCTGTCTCTTAAGCACACCTCAGCGGGG CCTCGCTACCAATACTTGATTAACCACAAGGAAAAGTGTCAAGCTCAAGACGTCCTCCTTTTACTGTTTG TAAAAACTGCTCCTGAAAACTATGATCGACGTTCCGGAATTAGAAGGACGTGGGGCAATGAAAATTATGT TCGGTCTCAGCTGAATGCCAACATCAAAACTCTGTTTGCCTTAGGAACTCCTAATCCACTGGAGGGAGAA GAACTACAAAGAAAACTGGCTTGGGAAGATCAAAGGTACAATGATATAATTCAGCAAGACTTTGTTGATT CTTTCTACAATCTTACTCTGAAATTACTTATGCAGTTCAGTTGGGCAAATACCTATTGTCCACATGCCAA ATTTCTTATGACTGCTGATGATGACATATTTATTCACATGCCAAATCTGATTGAGTACCTTCAAAGTTTA GAACAAATTGGTGTTCAAGACTTTTGGATTGGTCGTGTTCATCGTGGTGCCCCTCCCATTAGAGATAAAA GCAGCAAATACTACGTGTCCTATGAAATGTACCAGTGGCCAGCTTACCCTGACTACACAGCCGGAGCTGC CTATGTAATCTCCGGTGATGTAGCTGCCAAAGTCTATGAGGCATCACAGACACTAAATTCAAGTCTTTAC ATAGACGATGTGTTCATGGGCCTCTGTGCCAATAAAATAGGGATAGTACCGCAGGACCATGTGTTTTTTT CTGGAGAGGGTAAAACTCCTTATCATCCCTGCATCTATGAAAAAATGATGACATCTCATGGACACTTAGA AGATCTCCAGGACCTTTGGAAGAATGCTACAGATCCTAAAGTAAAAACCATTTCCAAAGGTTTTTTTGGT CAAATATACTGCAGATTAATGAAGATAATTCTCCTTTGTAAAATTAGCTATGTGGACACATACCCTTGTA GGGCTGCGTTTATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206965 protein sequence
Red=Cloning site Green=Tags(s) MRMLVSGRRVKKWQLIIQLFATCFLASLMFFWEPIDNHIVSHMKSYSYRYLINSYDFVNDTLSLKHTSAG PRYQYLINHKEKCQAQDVLLLLFVKTAPENYDRRSGIRRTWGNENYVRSQLNANIKTLFALGTPNPLEGE ELQRKLAWEDQRYNDIIQQDFVDSFYNLTLKLLMQFSWANTYCPHAKFLMTADDDIFIHMPNLIEYLQSL EQIGVQDFWIGRVHRGAPPIRDKSSKYYVSYEMYQWPAYPDYTAGAAYVISGDVAAKVYEASQTLNSSLY IDDVFMGLCANKIGIVPQDHVFFSGEGKTPYHPCIYEKMMTSHGHLEDLQDLWKNATDPKVKTISKGFFG QIYCRLMKIILLCKISYVDTYPCRAAFI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032047 |
| ORF Size | 1134 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032047.5 |
| RefSeq Size | 4131 bp |
| RefSeq ORF | 1137 bp |
| Locus ID | 84002 |
| UniProt ID | Q9BYG0 |
| Domains | Galactosyl_T |
| Protein Families | Transmembrane |
| Protein Pathways | Glycosphingolipid biosynthesis - lacto and neolacto series, Metabolic pathways |
| MW | 44.1 kDa |
| Gene Summary | This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II membrane protein. It exhibits strong activity to transfer GlcNAc to glycolipid substrates and is identified as the most likely candidate for lactotriaosylceramide synthase. This enzyme is essential for the expression of Lewis X epitopes on glycolipids. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206965L1 | Lenti ORF clone of Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206965L2 | Lenti ORF clone of Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5), mGFP tagged |
CNY 5,890.00 |
|
| RC206965L3 | Lenti ORF clone of Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206965L4 | Lenti ORF clone of Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5), mGFP tagged |
CNY 5,890.00 |
|
| RG206965 | B3GNT5 (tGFP-tagged) - Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5) |
CNY 4,370.00 |
|
| SC106413 | B3GNT5 (untagged)-Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5) |
CNY 2,950.00 |
|
| SC323946 | B3GNT5 (untagged)-Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5) |
CNY 3,656.00 |
