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E3 SUMO protein ligase PIAS4 (PIAS4) (NM_015897) Human Tagged ORF Clone
CAT＃: RC206748
PIAS4 (Myc-DDK-tagged)-Human protein inhibitor of activated STAT, 4 (PIAS4)
ORF Plasmid: tGFP
"NM_015897" in other vectors (5)
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CNY 3,800.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PIAS-gamma; Piasg; PIASY; ZMIZ6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206748 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGAGCTGGTGGAGGCCAAAAACATGGTGATGAGTTTTCGAGTCTCCGACCTTCAGATGCTCC TGGGTTTCGTGGGCCGGAGTAAGAGTGGACTGAAGCACGAGCTCGTCACCAGGGCCCTCCAGCTGGTGCA GTTTGACTGTAGCCCTGAGCTGTTCAAGAAGATCAAGGAGCTGTACGAGACCCGCTACGCCAAGAAGAAC TCGGAGCCTGCCCCACAGCCGCACCGGCCCCTGGACCCCCTGACCATGCACTCCACCTACGACCGGGCCG GCGCTGTGCCCAGGACTCCGCTGGCAGGCCCCAATATTGACTACCCCGTGCTCTACGGAAAGTACTTAAA CGGACTGGGACGGTTGCCCGCCAAGACCCTCAAGCCAGAAGTCCGCCTGGTGAAGCTGCCGTTCTTTAAT ATGCTGGATGAGCTGCTGAAGCCCACCGAATTAGTCCCACAGAACAACGAGAAGCTTCAGGAGAGCCCGT GCATCTTCGCATTGACGCCAAGACAGGTGGAGTTGATCCGGAACTCCAGGGAACTGCAGCCCGGAGTTAA AGCCGTGCAGGTCGTCCTGAGAATCTGTTACTCAGACACCAGCTGCCCTCAGGAGGACCAGTACCCGCCC AACATCGCTGTGAAGGTCAACCACAGCTACTGCTCCGTCCCGGGCTACTACCCCTCCAATAAGCCCGGGG TGGAGCCCAAGAGGCCGTGCCGCCCCATCAACCTCACTCACCTCATGTACCTGTCCTCGGCCACCAACCG CATCACTGTCACCTGGGGGAACTACGGCAAGAGCTACTCGGTGGCCCTGTACCTGGTGCGGCAGCTGACC TCATCGGAGCTGCTGCAGAGGCTGAAGACCATTGGGGTAAAGCACCCGGAGCTGTGCAAGGCACTGGTCA AGGAGAAGCTGCGCCTTGATCCTGACAGCGAGATCGCCACCACCGGTGTGCGGGTGTCCCTCATCTGTCC GCTGGTGAAGATGCGGCTCTCCGTGCCCTGCCGGGCAGAGACCTGCGCCCACCTGCAGTGCTTCGACGCC GTCTTCTACCTGCAGATGAACGAGAAGAAGCCCACCTGGATGTGCCCCGTGTGCGACAAGCCAGCCCCCT ACGACCAGCTCATCATCGACGGGCTCCTCTCGAAGATCCTGAGCGAGTGTGAGGACGCCGACGAGATCGA GTACCTGGTGGACGGCTCGTGGTGCCCGATCCGCGCCGAAAAGGAGCGCAGCTGCAGCCCGCAGGGCGCC ATCCTCGTGCTGGGCCCCTCGGACGCCAATGGGCTCCTGCCCGCCCCCAGCGTCAACGGGAGCGGTGCCC TGGGCAGCACGGGTGGCGGCGGCCCGGTGGGCAGCATGGAGAATGGGAAGCCGGGCGCCGATGTGGTGGA CCTCACGCTGGACAGCTCATCGTCCTCGGAGGATGAGGAGGAGGAGGAAGAGGAGGAGGAAGACGAGGAC GAAGAGGGGCCCCGGCCCAAGCGCCGCTGCCCCTTCCAGAAGGGCCTGGTGCCGGCCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206748 protein sequence
Red=Cloning site Green=Tags(s) MAAELVEAKNMVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLVQFDCSPELFKKIKELYETRYAKKN SEPAPQPHRPLDPLTMHSTYDRAGAVPRTPLAGPNIDYPVLYGKYLNGLGRLPAKTLKPEVRLVKLPFFN MLDELLKPTELVPQNNEKLQESPCIFALTPRQVELIRNSRELQPGVKAVQVVLRICYSDTSCPQEDQYPP NIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRITVTWGNYGKSYSVALYLVRQLT SSELLQRLKTIGVKHPELCKALVKEKLRLDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDA VFYLQMNEKKPTWMCPVCDKPAPYDQLIIDGLLSKILSECEDADEIEYLVDGSWCPIRAEKERSCSPQGA ILVLGPSDANGLLPAPSVNGSGALGSTGGGGPVGSMENGKPGADVVDLTLDSSSSSEDEEEEEEEEEDED EEGPRPKRRCPFQKGLVPAC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015897 |
| ORF Size | 1530 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015897.3 |
| RefSeq Size | 1835 bp |
| RefSeq ORF | 1533 bp |
| Locus ID | 51588 |
| UniProt ID | Q8N2W9 |
| Domains | SAP, zf-MIZ |
| Protein Families | Transcription Factors |
| Protein Pathways | Jak-STAT signaling pathway, Pathways in cancer, Small cell lung cancer, Ubiquitin mediated proteolysis |
| MW | 56.5 kDa |
| Gene Summary | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206748L3 | Lenti ORF clone of Human protein inhibitor of activated STAT, 4 (PIAS4), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206748L4 | Lenti ORF clone of Human protein inhibitor of activated STAT, 4 (PIAS4), mGFP tagged |
CNY 5,890.00 |
|
| RG206748 | PIAS4 (tGFP-tagged) - Human protein inhibitor of activated STAT, 4 (PIAS4) |
CNY 4,370.00 |
|
| SC114580 | PIAS4 (untagged)-Human protein inhibitor of activated STAT, 4 (PIAS4) |
CNY 2,950.00 |
|
| SC324286 | PIAS4 (untagged)-Human protein inhibitor of activated STAT, 4 (PIAS4) |
CNY 2,950.00 |
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