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SCNN1B (NM_000336) Human Tagged ORF Clone
CAT＃: RC206580
SCNN1B (Myc-DDK-tagged)-Human sodium channel, nonvoltage-gated 1, beta (SCNN1B)
ORF Plasmid: tGFP
"NM_000336" in other vectors (4)
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CNY 4,560.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BESC1; ENaCb; ENaCbeta; LIDLS1; SCNEB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206580 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACGTGAAGAAGTACCTGCTGAAGGGCCTGCATCGGCTGCAGAAGGGCCCCGGCTACACGTACAAGG AGCTGCTGGTGTGGTACTGCGACAACACCAACACCCACGGCCCCAAGCGCACCATCTGTGAGGGGCCCAA GAAGAAAGCCATGTGGTTCCTGCTCACCCTGCTCTTCGCCGCCCTCGTCTGCTGGCAGTGGGGCATCTTC ATCAGGACCTACTTGAGCTGGGAGGTCAGCGTCTCCCTCTCCGTAGGCTTCAAGACCATGGACTTCCCTG CCGTCACCATCTGCAATGCTAGCCCCTTCAAGTATTCCAAAATCAAGCATTTGCTGAAGGACCTGGATGA GCTGATGGAAGCTGTCCTGGAGAGAATCCTGGCTCCTGAGCTAAGCCATGCCAATGCCACCAGGAACCTG AACTTCTCCATCTGGAACCACACACCCCTGGTCCTTATTGATGAACGGAACCCCCACCACCCCATGGTCC TTGATCTCTTTGGAGACAACCACAATGGCTTAACAAGCAGCTCAGCATCAGAAAAGATCTGTAATGCCCA CGGGTGCAAAATGGCCATGAGACTATGTAGCCTCAACAGGACCCAGTGTACCTTCCGGAACTTCACCAGT GCTACCCAGGCATTGACAGAGTGGTACATCCTGCAGGCCACCAACATCTTTGCACAGGTGCCACAGCAGG AGCTAGTAGAGATGAGCTACCCCGGCGAGCAGATGATCCTGGCCTGCCTATTCGGAGCTGAGCCCTGCAA CTACCGGAACTTCACGTCCATCTTCTACCCTCACTATGGCAACTGTTACATCTTCAACTGGGGCATGACA GAGAAGGCACTTCCTTCGGCCAACCCTGGAACTGAATTCGGCCTGAAGTTGATCCTGGACATAGGCCAGG AAGACTACGTCCCCTTCCTTGCGTCCACGGCCGGGGTCAGGCTGATGCTTCACGAGCAGAGGTCATACCC CTTCATCAGAGATGAGGGCATCTACGCCATGTCGGGGACAGAGACGTCCATCGGGGTACTCGTGGACAAG CTTCAGCGCATGGGGGAGCCCTACAGCCCGTGCACCGTGAATGGTTCTGAGGTCCCCGTCCAAAACTTCT ACAGTGACTACAACACGACCTACTCCATCCAGGCCTGTCTTCGCTCCTGCTTCCAAGACCACATGATCCG TAACTGCAACTGTGGCCACTACCTGTACCCACTGCCCCGTGGGGAGAAATACTGCAACAACCGGGACTTC CCAGACTGGGCCCATTGCTACTCAGATCTACAGATGAGCGTGGCGCAGAGAGAGACCTGCATTGGCATGT GCAAGGAGTCCTGCAATGACACCCAGTACAAGATGACCATCTCCATGGCTGACTGGCCTTCTGAGGCCTC CGAGGACTGGATTTTCCACGTCTTGTCTCAGGAGCGGGACCAAAGCACCAATATCACCCTGAGCAGGAAG GGAATTGTCAAGCTCAACATCTACTTCCAAGAATTTAACTATCGCACCATTGAAGAATCAGCAGCCAATA ACATCGTCTGGCTGCTCTCGAATCTGGGTGGCCAGTTTGGCTTCTGGATGGGGGGCTCTGTGCTGTGCCT CATCGAGTTTGGGGAGATCATCATCGACTTTGTGTGGATCACCATCATCAAGCTGGTGGCCTTGGCCAAG AGCCTACGGCAGCGGCGAGCCCAAGCCAGCTACGCTGGCCCACCGCCCACCGTGGCCGAGCTGGTGGAGG CCCACACCAACTTTGGCTTCCAGCCTGACACGGCCCCCCGCAGCCCCAACACTGGGCCCTACCCCAGTGA GCAGGCCCTGCCCATCCCAGGCACCCCGCCCCCCAACTATGACTCCCTGCGTCTGCAGCCGCTGGACGTC ATCGAGTCTGACAGTGAGGGTGATGCCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206580 protein sequence
Red=Cloning site Green=Tags(s) MHVKKYLLKGLHRLQKGPGYTYKELLVWYCDNTNTHGPKRTICEGPKKKAMWFLLTLLFAALVCWQWGIF IRTYLSWEVSVSLSVGFKTMDFPAVTICNASPFKYSKIKHLLKDLDELMEAVLERILAPELSHANATRNL NFSIWNHTPLVLIDERNPHHPMVLDLFGDNHNGLTSSSASEKICNAHGCKMAMRLCSLNRTQCTFRNFTS ATQALTEWYILQATNIFAQVPQQELVEMSYPGEQMILACLFGAEPCNYRNFTSIFYPHYGNCYIFNWGMT EKALPSANPGTEFGLKLILDIGQEDYVPFLASTAGVRLMLHEQRSYPFIRDEGIYAMSGTETSIGVLVDK LQRMGEPYSPCTVNGSEVPVQNFYSDYNTTYSIQACLRSCFQDHMIRNCNCGHYLYPLPRGEKYCNNRDF PDWAHCYSDLQMSVAQRETCIGMCKESCNDTQYKMTISMADWPSEASEDWIFHVLSQERDQSTNITLSRK GIVKLNIYFQEFNYRTIEESAANNIVWLLSNLGGQFGFWMGGSVLCLIEFGEIIIDFVWITIIKLVALAK SLRQRRAQASYAGPPPTVAELVEAHTNFGFQPDTAPRSPNTGPYPSEQALPIPGTPPPNYDSLRLQPLDV IESDSEGDAI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000336 |
| ORF Size | 1920 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000336.3 |
| RefSeq Size | 2597 bp |
| RefSeq ORF | 1923 bp |
| Locus ID | 6338 |
| UniProt ID | P51168 |
| Domains | ASC |
| Protein Families | Druggable Genome, Ion Channels: Other, Transmembrane |
| Protein Pathways | Taste transduction |
| MW | 72.6 kDa |
| Gene Summary | Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the beta subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), and Liddle syndrome. [provided by RefSeq, Apr 2009] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
High resolution measurement of membrane receptor endocytosis
,Zhang, Z;Heidary, DK;Richards, CI;,
Journal of Biological Methods
[SCNN1B]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206580L3 | Lenti ORF clone of Human sodium channel, nonvoltage-gated 1, beta (SCNN1B), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC206580L4 | Lenti ORF clone of Human sodium channel, nonvoltage-gated 1, beta (SCNN1B), mGFP tagged |
CNY 6,460.00 |
|
| RG206580 | SCNN1B (tGFP-tagged) - Human sodium channel, nonvoltage-gated 1, beta (SCNN1B) |
CNY 5,040.00 |
|
| SC119979 | SCNN1B (untagged)-Human sodium channel, nonvoltage-gated 1, beta (SCNN1B) |
CNY 2,950.00 |
