United States
Germany
Japan
United Kingdom
China
ALG1 (NM_019109) Human Tagged ORF Clone
CAT＃: RC206343
ALG1 (Myc-DDK-tagged)-Human asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae) (ALG1)
ORF Plasmid: tGFP
"NM_019109" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CDG1K; hMat-1; HMAT1; HMT-1; HMT1; Mat-1; MT-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206343 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCTCATGCTTGGTCCTGCTGGCGCTGTGTCTGCTGCTGCCGCTGCTGCTGCTGGGAGGATGGA AGCGCTGGCGCCGGGGGCGGGCGGCCCGGCATGTAGTAGCGGTGGTGCTGGGCGACGTGGGCCGCAGCCC CCGTATGCAGTACCACGCGCTGTCGTTGGCCATGCACGGCTTCTCGGTGACCCTCCTGGGGTTCTGCAAC TCCAAACCCCATGATGAGCTCTTGCAGAACAACAGAATTCAGATTGTGGGGTTGACAGAACTTCAGAGTC TTGCAGTTGGGCCCCGAGTTTTCCAGTACGGAGTCAAAGTTGTACTTCAGGCTATGTACTTGCTGTGGAA GTTGATGTGGAGGGAGCCAGGTGCCTATATCTTTCTCCAGAACCCCCCAGGTCTGCCTAGCATTGCTGTC TGCTGGTTCGTGGGCTGCCTTTGTGGAAGCAAGCTCGTCATTGACTGGCACAACTATGGCTACTCCATCA TGGGTCTGGTGCATGGCCCCAACCATCCCCTCGTTCTGCTGGCCAAGTGGTACGAGAAGTTCTTTGGGCG CCTGTCCCACCTGAACCTGTGTGTTACCAATGCTATGCGAGAAGACCTGGCGGATAACTGGCACATCAGG GCTGTGACCGTCTACGACAAGCCCGCATCTTTCTTTAAAGAGACACCTCTGGACCTGCAGCACCGGCTCT TCATGAAGCTGGGCAGCATGCACTCTCCGTTCAGGGCCCGCTCAGAACCTGAGGACCCAGTCACGGAGCG GTCGGCCTTCACGGAGCGGGATGCTGGGAGCGGGCTGGTGACGCGTCTCCGTGAGCGGCCAGCCCTGCTG GTCAGCAGCACGAGCTGGACAGAGGACGAAGACTTCTCCATCCTGCTGGCAGCTTTAGAAAAGTTTGAAC AACTGACTCTTGATGGACACAACCTTCCTTCTCTCGTCTGTGTGATAACAGGCAAAGGGCCTATGAGGGA GTATTATAGCCGCCTCATCCACCAGAAGCACTTCCAGCACATCCAGGTCTGCACCCCCTGGCTGGAGGCC GAGGACTACCCCCTGCTTCTAGGGTCGGCGGACCTGGGTGTCTGTCTGCACACGTCCTCCAGTGGCCTGG ACCTGCCCATGAAGGTGGTGGACATGTTCGGGTGCTGTTTGCCTGTGTGTGCTGTGAACTTCAAGTGTTT ACATGAGCTGGTGAAACATGAAGAAAATGGCCTGGTCTTTGAGGACTCAGAGGAACTGGCAGCTCAGCTG CAGATGCTTTTCTCAAACTTTCCTGATCCTGCGGGCAAGCTAAACCAGTTCCGGAAGAACCTGCGGGAGT CGCAGCAGCTCCGATGGGATGAGAGCTGGGTGCGGACTGTGCTCCCTTTGGTTATGGACACA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC206343 protein sequence
Red=Cloning site Green=Tags(s) MAASCLVLLALCLLLPLLLLGGWKRWRRGRAARHVVAVVLGDVGRSPRMQYHALSLAMHGFSVTLLGFCN SKPHDELLQNNRIQIVGLTELQSLAVGPRVFQYGVKVVLQAMYLLWKLMWREPGAYIFLQNPPGLPSIAV CWFVGCLCGSKLVIDWHNYGYSIMGLVHGPNHPLVLLAKWYEKFFGRLSHLNLCVTNAMREDLADNWHIR AVTVYDKPASFFKETPLDLQHRLFMKLGSMHSPFRARSEPEDPVTERSAFTERDAGSGLVTRLRERPALL VSSTSWTEDEDFSILLAALEKFEQLTLDGHNLPSLVCVITGKGPMREYYSRLIHQKHFQHIQVCTPWLEA EDYPLLLGSADLGVCLHTSSSGLDLPMKVVDMFGCCLPVCAVNFKCLHELVKHEENGLVFEDSEELAAQL QMLFSNFPDPAGKLNQFRKNLRESQQLRWDESWVRTVLPLVMDT SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019109 |
| ORF Size | 1392 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019109.2 |
| RefSeq Size | 3946 bp |
| RefSeq ORF | 1395 bp |
| Locus ID | 56052 |
| UniProt ID | Q9BT22 |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
| MW | 52.6 kDa |
| Gene Summary | The enzyme encoded by this gene catalyzes the first mannosylation step in the biosynthesis of lipid-linked oligosaccharides. This gene is mutated in congenital disorder of glycosylation type Ik. [provided by RefSeq, Dec 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
ALG1-CDG: Clinical and Molecular Characterization of 39 Unreported Patients
,Ng, BG;Shiryaev, SA;Rymen, D;Eklund, EA;Raymond, K;Kircher, M;Abdenur, JE;Alehan, F;Midro, AT;Bamshad, MJ;Barone, R;Berry, GT;Brumbaugh, JE;Buckingham, KJ;Clarkson, K;Cole, FS;O'Connor, S;Cooper, GM;Van Coster, R;Demmer, LA;Diogo, L;Fay, AJ;Ficicioglu, C;Fiumara, A;Gahl, WA;Ganetzky, R;Goel, H;Harshman, LA;He, M;Jaeken, J;James, PM;Katz, D;Keldermans, L;Kibaek, M;Kornberg, AJ;Lachlan, K;Lam, C;Yaplito-Lee, J;Nickerson, DA;Peters, HL;Race, V;Régal, L;Rush, JS;Rutledge, SL;Shendure, J;Souche, E;Sparks, SE;Trapane, P;Sanchez-Valle, A;Vilain, E;Vøllo, A;Waechter, CJ;Wang, RY;Wolfe, LA;Wong, DA;Wood, T;Yang, AC;University of Washington Center for Mendelian Genomics, ;Matthijs, G;Freeze, HH;,
Hum. Mutat.
,PubMed ID 26931382
[ALG1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206343L3 | Lenti ORF clone of Human asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae) (ALG1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206343L4 | Lenti ORF clone of Human asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae) (ALG1), mGFP tagged |
CNY 5,890.00 |
|
| RG206343 | ALG1 (tGFP-tagged) - Human asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae) (ALG1) |
CNY 4,370.00 |
|
| SC122883 | ALG1 (untagged)-Human asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae) (ALG1) |
CNY 2,950.00 |
