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GABA A Receptor alpha 3 (GABRA3) (NM_000808) Human Tagged ORF Clone
CAT＃: RC206286
GABRA3 (Myc-DDK-tagged)-Human gamma-aminobutyric acid (GABA) A receptor, alpha 3 (GABRA3)
ORF Plasmid: tGFP
"NM_000808" in other vectors (4)
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CNY 5,488.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206286 representing NM_000808
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATAATCACACAAACAAGTCACTGTTACATGACCAGCCTTGGGATTCTTTTCCTGATTAATATTCTCC CTGGAACCACTGGTCAAGGGGAATCAAGACGACAAGAACCCGGGGACTTTGTGAAGCAGGACATTGGCGG GCTGTCTCCTAAGCATGCCCCAGATATTCCTGATGACAGCACTGACAACATCACTATCTTCACCAGAATC TTGGATCGTCTTCTGGACGGCTATGACAACCGGCTGCGACCTGGGCTTGGAGATGCAGTGACTGAAGTGA AGACTGACATCTACGTGACCAGTTTTGGCCCTGTGTCAGACACTGACATGGAGTACACTATTGATGTATT TTTTCGGCAGACATGGCATGATGAAAGACTGAAATTTGATGGCCCCATGAAGATCCTTCCACTGAACAAT CTCCTGGCTAGTAAGATCTGGACACCGGACACCTTCTTCCACAATGGCAAGAAATCAGTGGCTCATAACA TGACCACGCCCAACAAGCTGCTCAGATTGGTGGACAACGGAACCCTCCTCTATACAATGAGGTTAACAAT TCATGCTGAGTGTCCCATGCATTTGGAAGATTTTCCCATGGATGTGCATGCCTGCCCACTGAAGTTTGGA AGCTATGCCTATACAACAGCTGAAGTGGTTTATTCTTGGACTCTCGGAAAGAACAAATCCGTGGAAGTGG CACAGGATGGTTCTCGCTTGAACCAGTATGACCTTTTGGGCCATGTTGTTGGGACAGAGATAATCCGGTC TAGTACAGGAGAATATGTCGTCATGACAACCCACTTCCATCTCAAGCGAAAAATTGGCTACTTTGTGATC CAGACCTACTTGCCATGTATCATGACTGTCATTCTGTCACAAGTGTCGTTCTGGCTCAACAGAGAGTCTG TTCCTGCCCGTACAGTCTTTGGTGTCACCACTGTGCTTACCATGACCACCTTGAGTATCAGTGCCAGAAA TTCCTTACCTAAAGTGGCATATGCGACGGCCATGGACTGGTTCATAGCCGTCTGTTATGCCTTTGTATTT TCTGCACTGATTGAATTTGCCACTGTCAACTATTTCACCAAGCGGAGTTGGGCTTGGGAAGGCAAGAAGG TGCCAGAGGCCCTGGAGATGAAGAAGAAAACACCAGCAGCCCCAGCAAAGAAAACCAGCACTACCTTCAA CATCGTGGGGACCACCTATCCCATCAACCTGGCCAAGGACACTGAATTTTCCACCATCTCCAAGGGCGCT GCTCCCAGTGCCTCCTCAACCCCAACAATCATTGCTTCACCCAAGGCCACCTACGTGCAGGACAGCCCGA CTGAGACCAAGACCTACAACAGTGTCAGCAAGGTTGACAAAATTTCCCGCATCATCTTTCCTGTGCTCTT TGCCATATTCAATCTGGTCTATTGGGCCACATATGTCAACCGGGAGTCAGCTATCAAGGGCATGATCCGC AAACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206286 representing NM_000808
Red=Cloning site Green=Tags(s) MIITQTSHCYMTSLGILFLINILPGTTGQGESRRQEPGDFVKQDIGGLSPKHAPDIPDDSTDNITIFTRI LDRLLDGYDNRLRPGLGDAVTEVKTDIYVTSFGPVSDTDMEYTIDVFFRQTWHDERLKFDGPMKILPLNN LLASKIWTPDTFFHNGKKSVAHNMTTPNKLLRLVDNGTLLYTMRLTIHAECPMHLEDFPMDVHACPLKFG SYAYTTAEVVYSWTLGKNKSVEVAQDGSRLNQYDLLGHVVGTEIIRSSTGEYVVMTTHFHLKRKIGYFVI QTYLPCIMTVILSQVSFWLNRESVPARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVF SALIEFATVNYFTKRSWAWEGKKVPEALEMKKKTPAAPAKKTSTTFNIVGTTYPINLAKDTEFSTISKGA APSASSTPTIIASPKATYVQDSPTETKTYNSVSKVDKISRIIFPVLFAIFNLVYWATYVNRESAIKGMIR KQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000808 |
| ORF Size | 1476 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000808.4 |
| RefSeq Size | 1901 bp |
| RefSeq ORF | 1479 bp |
| Locus ID | 2556 |
| UniProt ID | P34903 |
| Domains | Neur_chan_memb, Neur_chan_LBD |
| Protein Families | Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| MW | 55 kDa |
| Gene Summary | GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Global RNA editome landscape discovers reduced RNA editing in glioma: loss of editing of gamma-amino butyric acid receptor alpha subunit 3 (GABRA3) favors glioma migration and invasion
,null,
PeerJ
,PubMed ID 33062411
[GABA A Receptor alpha 3]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206286L3 | Lenti-ORF clone of GABRA3 (Myc-DDK-tagged)-Human gamma-aminobutyric acid (GABA) A receptor, alpha 3 (GABRA3) |
CNY 5,890.00 |
|
| RC206286L4 | Lenti-ORF clone of GABRA3 (mGFP-tagged)-Human gamma-aminobutyric acid (GABA) A receptor, alpha 3 (GABRA3) |
CNY 5,890.00 |
|
| RG206286 | GABRA3 (tGFP-tagged) - Human gamma-aminobutyric acid (GABA) A receptor, alpha 3 (GABRA3) |
CNY 7,088.00 |
|
| SC119670 | GABRA3 (untagged)-Human gamma-aminobutyric acid (GABA) A receptor, alpha 3 (GABRA3) |
CNY 5,488.00 |
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