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GUCY1A1 (NM_000856) Human Tagged ORF Clone
CAT＃: RC206063
GUCY1A3 (Myc-DDK-tagged)-Human guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant 1
ORF Plasmid: tGFP
"NM_000856" in other vectors (6)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3; GUCA3; GUCSA3; GUCY1A3; MYMY6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206063 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTGCACGAAGCTCAAGGATCTCAAGATCACAGGAGAGTGTCCTTTCTCCTTACTGGCACCAGGTC AAGTTCCTAACGAGTCTTCAGAGGAGGCAGCAGGAAGCTCAGAGAGCTGCAAAGCAACCATGCCCATCTG TCAAGACATTCCTGAGAAGAACATACAAGAAAGTCTTCCTCAAAGAAAAACCAGTCGGAGCCGAGTCTAT CTTCACACTTTGGCAGAGAGTATTTGCAAACTGATTTTCCCAGAGTTTGAACGGCTGAATGTTGCACTTC AGAGAACATTGGCAAAGCACAAAATAAAAGAAAGCAGGAAATCTTTGGAAAGAGAAGACTTTGAAAAAAC AATTGCAGAGCAAGCAGTTGCAGCAGGAGTTCCAGTGGAGGTTATCAAAGAATCTCTTGGTGAAGAGGTT TTTAAAATATGTTACGAGGAAGATGAAAACATCCTTGGGGTGGTTGGAGGCACCCTTAAAGATTTTTTAA ACAGCTTCAGTACCCTTCTGAAACAGAGCAGCCATTGCCAAGAAGCAGGAAAAAGGGGCAGGCTTGAGGA CGCCTCCATTCTATGCCTGGATAAGGAGGATGATTTTCTACATGTTTACTACTTCTTCCCTAAGAGAACC ACCTCCCTGATTCTTCCCGGCATCATAAAGGCAGCTGCTCACGTATTATATGAAACGGAAGTGGAAGTGT CGTTAATGCCTCCCTGCTTCCATAATGATTGCAGCGAGTTTGTGAATCAGCCCTACTTGTTGTACTCCGT TCACATGAAAAGCACCAAGCCATCCCTGTCCCCCAGCAAACCCCAGTCCTCGCTGGTGATTCCCACATCG CTATTCTGCAAGACATTTCCATTCCATTTCATGTTTGACAAAGATATGACAATTCTGCAATTTGGCAATG GCATCAGAAGGCTGATGAACAGGAGAGACTTTCAAGGAAAGCCTAATTTTGAAGAATACTTTGAAATTCT GACTCCAAAAATCAACCAGACGTTTAGCGGGATCATGACTATGTTGAATATGCAGTTTGTTGTACGAGTG AGGAGATGGGACAACTCTGTGAAGAAATCTTCAAGGGTTATGGACCTCAAAGGCCAAATGATCTACATTG TTGAATCCAGTGCAATCTTGTTTTTGGGGTCACCCTGTGTGGACAGATTAGAAGATTTTACAGGACGAGG GCTCTACCTCTCAGACATCCCAATTCACAATGCACTGAGGGATGTGGTCTTAATAGGGGAACAAGCCCGA GCTCAAGATGGCCTGAAGAAGAGGCTGGGGAAGCTGAAGGCTACCCTTGAGCAAGCCCACCAAGCCCTGG AGGAGGAGAAGAAAAAGACAGTAGACCTTCTGTGCTCCATATTTCCCTGTGAGGTTGCTCAGCAGCTGTG GCAAGGGCAAGTTGTGCAAGCCAAGAAGTTCAGTAATGTCACCATGCTCTTCTCAGACATCGTTGGGTTC ACTGCCATCTGCTCCCAGTGCTCACCGCTGCAGGTCATCACCATGCTCAATGCACTGTACACTCGCTTCG ACCAGCAGTGTGGAGAGCTGGATGTCTACAAGGTGGAGACCATTGGCGATGCCTATTGTGTAGCTGGGGG ATTACACAAAGAGAGTGATACTCATGCTGTTCAGATAGCGCTGATGGCCGTGAAGATGATGGAGCTCTCT GATGAAGTTATGTCTCCCCATGGAGAACCTATCAAGATGCGAATTGGACTGCACTCTGGATCAGTTTTTG CTGGCGTCGTTGGAGTTAAAATGCCCCGTTACTGTCTTTTTGGAAACAATGTCACTCTGGCTAACAAATT TGAGTCCTGCAGTGTACCACGAAAAATCAATGTCAGCCCAACAACTTACAGATTACTCAAAGACTGTCCT GGTTTCGTGTTTACCCCTCGATCAAGGGAGGAACTTCCACCAAACTTCCCTAGTGAAATCCCCGGAATCT GCCATTTTCTGGATGCTTACCAACAAGGAACAAACTCAAAACCATGCTTCCAAAAGAAAGATGTGGAAGA TGGCAATGCCAATTTTTTAGGCAAAGCATCAGGAATAGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206063 protein sequence
Red=Cloning site Green=Tags(s) MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATMPICQDIPEKNIQESLPQRKTSRSRVY LHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEV FKICYEEDENILGVVGGTLKDFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRT TSLILPGIIKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTS LFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRV RRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQAR AQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGF TAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMAVKMMELS DEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCP GFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000856 |
| ORF Size | 2070 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000856.6 |
| RefSeq Size | 9454 bp |
| RefSeq ORF | 2073 bp |
| Locus ID | 2982 |
| UniProt ID | Q02108 |
| Domains | CYCc |
| Protein Families | Druggable Genome |
| Protein Pathways | Gap junction, Long-term depression, Purine metabolism, Vascular smooth muscle contraction |
| MW | 77.5 kDa |
| Gene Summary | Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206063L1 | Lenti ORF clone of Human guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206063L2 | Lenti ORF clone of Human guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC206063L3 | Lenti ORF clone of Human guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206063L4 | Lenti ORF clone of Human guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG206063 | GUCY1A3 (tGFP-tagged) - Human guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant 1 |
CNY 4,370.00 |
|
| SC119658 | GUCY1A3 (untagged)-Human guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant 1 |
CNY 5,064.00 |
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