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PAPOLA (NM_032632) Human Tagged ORF Clone
CAT＃: RC205956
PAPOLA (Myc-DDK-tagged)-Human poly(A) polymerase alpha (PAPOLA)
ORF Plasmid: tGFP
"NM_032632" in other vectors (6)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PAP; PAP-alpha |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205956 representing NM_032632
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGTTTCCAGTTACAACACAGGGATCACAACAAACACAACCGCCACAGAAGCACTATGGCATTACTT CTCCTATCAGCTTAGCAGCCCCCAAGGAGACTGACTGCGTACTTACACAGAAACTAATTGAGACATTGAA ACCCTTTGGGGTTTTTGAAGAGGAAGAGGAACTGCAGCGCAGGATTTTAATTTTGGGAAAACTAAATAAC CTGGTAAAAGAGTGGATACGAGAAATCAGTGAAAGCAAGAATCTTCCACAATCTGTAATTGAAAATGTTG GAGGAAAAATTTTTACATTTGGATCTTACAGATTAGGAGTGCATACAAAAGGTGCTGATATTGATGCGTT GTGTGTTGCACCAAGACATGTTGATCGAAGTGACTTTTTCACCTCATTCTATGATAAGTTGAAATTACAG GAAGAAGTAAAAGATTTAAGAGCTGTTGAAGAGGCATTCGTACCAGTTATTAAACTCTGTTTTGATGGGA TAGAGATTGATATTTTGTTTGCAAGATTAGCACTGCAGACAATTCCTGAAGATTTGGATCTACGAGATGA CAGTCTGCTAAAAAATTTAGATATAAGATGTATAAGAAGTCTTAACGGTTGCAGGGTAACCGATGAAATT TTACATCTAGTACCAAACATTGACAACTTCAGGTTAACTCTGAGAGCTATCAAACTATGGGCCAAACGCC ACAACATCTATTCCAATATATTAGGTTTCCTCGGTGGTGTTTCCTGGGCTATGCTAGTAGCAAGAACTTG CCAGCTTTATCCAAATGCAATAGCATCAACTCTTGTACATAAATTTTTCTTGGTATTTTCTAAATGGGAA TGGCCAAATCCAGTGCTATTGAAACAGCCTGAAGAATGCAATCTTAATTTGCCTGTATGGGACCCAAGGG TAAACCCCAGTGATAGGTACCATCTTATGCCTATAATTACACCAGCATACCCACAACAGAACTCCACGTA CAATGTGTCCGTTTCAACACGGATGGTCATGGTTGAGGAGTTTAAACAAGGTCTTGCTATCACAGATGAA ATTTTGCTGAGTAAGGCAGAGTGGTCCAAACTTTTTGAAGCTCCAAACTTCTTTCAAAAGTACAAGCATT ATATTGTACTTCTAGCAAGTGCACCAACAGAAAAACAACGCCTGGAATGGGTGGGCTTGGTGGAATCAAA AATCCGAATCCTGGTTGGAAGCTTGGAGAAGAATGAATTTATTACACTGGCTCATGTGAATCCCCAGTCA TTTCCAGCACCCAAAGAAAATCCCGACAAGGAAGAATTTCGCACGATGTGGGTGATTGGGTTAGTGTTTA AAAAAACAGAAAACTCTGAAAACCTCAGTGTTGATCTCACCTATGATATTCAGTCTTTCACAGATACAGT TTATAGGCAAGCAATAAACAGCAAGATGTTTGAGGTGGATATGAAAATTGCTGCAATGCATGTAAAAAGA AAGCAACTCCATCAACTACTACCTAATCATGTGCTTCAGAAAAAGAAAAAGCATTCAACAGAAGGTGTCA AATTGACAGCTCTCAATGACAGCAGCCTCGACTTGTCTATGGACAGTGATAACAGCATGTCTGTGCCTTC ACCTACTAGTGCTACGAAGACCAGTCCATTGAACAGTTCTGGCAGCTCTCAGGGCAGAAACAGTCCTGCT CCAGCTGTAACAGCAGCATCTGTGACCAACATACAGGCTACTGAAGTTTCTGTGCCACAAGTAAATTCCA GTGAAAGCTCAGGGGGTACATCGAGTGAAAGCATTCCTCAAACTGCCACACAACCAGCCATTTCTCCACC ACCAAAGCCTACGGTCTCCAGAGTTGTTTCTTCAACACGTCTGGTAAACCCACCACCTAGATCTTCAGGA AATGCAGCAACTTCAGGAAATGCAGCAACAAAAATACCTACTCCTATAGTAGGAGTCAAGAGGACATCCT CACCTCATAAAGAAGAGAGTCCCAAGAAAACCAAAACAGAAGAGGATGAAACAAGTGAAGATGCTAACTG TCTTGCTTTGAGTGGACATGATAAAACAGAAGCAAAGGAACAACTTGATACAGAGACAAGTACAACTCAA TCAGAAACTATTCAGACAGCGGCTTCTCTGTTGGCCTCTCAGAAAACATCCAGTACAGACCTTTCTGATA TCCCTGCTCTCCCTGCAAATCCTATTCCTGTTATCAAGAATTCAATAAAACTGAGATTGAATCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205956 representing NM_032632
Red=Cloning site Green=Tags(s) MPFPVTTQGSQQTQPPQKHYGITSPISLAAPKETDCVLTQKLIETLKPFGVFEEEEELQRRILILGKLNN LVKEWIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRSDFFTSFYDKLKLQ EEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEI LHLVPNIDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWE WPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDE ILLSKAEWSKLFEAPNFFQKYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQS FPAPKENPDKEEFRTMWVIGLVFKKTENSENLSVDLTYDIQSFTDTVYRQAINSKMFEVDMKIAAMHVKR KQLHQLLPNHVLQKKKKHSTEGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGRNSPA PAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSG NAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEDETSEDANCLALSGHDKTEAKEQLDTETSTTQ SETIQTAASLLASQKTSSTDLSDIPALPANPIPVIKNSIKLRLNR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032632 |
| ORF Size | 2235 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032632.4 |
| RefSeq Size | 4539 bp |
| RefSeq ORF | 2238 bp |
| Locus ID | 10914 |
| UniProt ID | P51003 |
| Domains | NTP_transf_2, PAP_central |
| Protein Families | Transcription Factors |
| Protein Pathways | RNA degradation |
| MW | 82.7 kDa |
| Gene Summary | The protein encoded by this gene belongs to the poly(A) polymerase family. It is required for the addition of adenosine residues for the creation of the 3'-poly(A) tail of mRNAs. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205956L1 | Lenti ORF clone of Human poly(A) polymerase alpha (PAPOLA), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205956L2 | Lenti ORF clone of Human poly(A) polymerase alpha (PAPOLA), mGFP tagged |
CNY 5,890.00 |
|
| RC205956L3 | Lenti ORF clone of Human poly(A) polymerase alpha (PAPOLA), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205956L4 | Lenti ORF clone of Human poly(A) polymerase alpha (PAPOLA), mGFP tagged |
CNY 5,890.00 |
|
| RG205956 | PAPOLA (tGFP-tagged) - Human poly(A) polymerase alpha (PAPOLA) |
CNY 4,370.00 |
|
| SC127314 | PAPOLA (untagged)-Human poly(A) polymerase alpha (PAPOLA) |
CNY 2,950.00 |
