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Ribophorin II (RPN2) (NM_002951) Human Tagged ORF Clone
CAT＃: RC205852
RPN2 (Myc-DDK-tagged)-Human ribophorin II (RPN2), transcript variant 1
ORF Plasmid: tGFP
"NM_002951" in other vectors (6)
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CNY 4,560.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RIBIIR; RPN-II; RPNII; SWP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205852 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCGCCGGGTTCAAGCACTGTCTTCCTGTTGGCCCTGACAATCATAGCCAGCACCTGGGCTCTGA CGCCCACTCACTACCTCACCAAGCATGACGTGGAGAGACTAAAAGCCTCGCTGGATCGCCCTTTCACAAA TTTGGAATCTGCCTTCTACTCCATCGTGGGACTCAGCAGCCTTGGTGCTCAGGTGCCAGATGCAAAGAAA GCATGTACCTACATCAGATCTAACCTTGATCCCAGCAATGTGGATTCCCTCTTCTACGCTGCCCAGGCCA GCCAGGCCCTCTCAGGATGTGAGATCTCTATTTCAAATGAGACCAAAGATCTGCTTCTGGCAGCTGTCAG TGAGGACTCATCTGTTACCCAGATCTACCATGCAGTTGCAGCTCTAAGTGGCTTTGGCCTTCCCTTGGCA TCCCAAGAAGCACTCAGTGCCCTTACTGCTCGTCTCAGCAAGGAGGAGACTGTGCTGGCAACAGTCCAGG CTCTGCAGACAGCATCCCACCTGTCCCAGCAGGCTGACCTGAGGAGCATCGTGGAGGAGATTGAGGACCT TGTTGCTCGCCTGGATGAACTCGGGGGCGTGTATCTCCAGTTTGAAGAAGGACTGGAAACAACAGCGTTA TTTGTGGCTGCCACCTACAAGCTCATGGATCATGTGGGGACTGAGCCATCCATTAAGGAGGATCAGGTCA TCCAGCTGATGAACGCGATCTTCAGCAAGAAGAACTTTGAGTCCCTCTCCGAAGCCTTCAGCGTGGCCTC TGCAGCTGCTGTGCTCTCGCATAATCGCTACCACGTGCCAGTTGTGGTTGTGCCTGAGGGCTCTGCTTCC GACACTCATGAACAGGCTATCTTGCGGTTGCAAGTCACCAATGTTCTGTCTCAGCCTCTGACTCAGGCCA CTGTTAAACTAGAACATGCTAAATCTGTTGCTTCCAGAGCCACTGTCCTCCAGAAGACATCCTTCACCCC TGTAGGGGATGTTTTTGAACTAAATTTCATGAACGTCAAATTTTCCAGTGGTTATTATGACTTCCTTGTC GAAGTTGAAGGTGACAACCGGTATATTGCAAATACCGTAGAGCTCAGAGTCAAGATCTCCACTGAAGTTG GCATCACAAATGTTGATCTTTCCACCGTGGATAAGGATCAGAGCATTGCACCCAAAACTACCCGGGTGAC ATACCCAGCCAAAGCCAAGGGCACATTCATCGCAGACAGCCACCAGAACTTCGCCTTGTTCTTCCAGCTG GTAGATGTGAACACTGGTGCTGAACTCACTCCTCACCAGACATTTGTCCGACTCCATAACCAGAAGACTG GCCAGGAAGTGGTGTTTGTTGCCGAGCCAGACAACAAGAACGTGTACAAGTTTGAACTGGATACCTCTGA AAGAAAGATTGAATTTGACTCTGCCTCTGGCACCTACACTCTCTACTTAATCATTGGAGATGCCACTTTG AAGAACCCAATCCTCTGGAATGTGGCTGATGTGGTCATCAAGTTCCCTGAGGAAGAAGCTCCCTCGACTG TCTTGTCCCAGAACCTTTTCACTCCAAAACAGGAAATTCAGCACCTGTTCCGCGAGCCTGAGAAGAGGCC CCCCACCGTGGTGTCCAATACATTCACTGCCCTGATCCTCTCGCCGTTGCTTCTGCTCTTCGCTCTGTGG ATCCGGATTGGTGCCAATGTCTCCAACTTCACTTTTGCTCCTAGCACGATTATATTTCACCTGGGACATG CTGCTATGCTGGGACTCATGTATGTCTACTGGACTCAGCTCAACATGTTCCAGACCTTGAAGTACCTGGC CATCTTGGGCAGTGTGACGTTTCTGGCTGGCAATCGGATGCTGGCCCAGCAGGCAGTCAAGAGAACAGCA CAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205852 protein sequence
Red=Cloning site Green=Tags(s) MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSLGAQVPDAKK ACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSSVTQIYHAVAALSGFGLPLA SQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSIVEEIEDLVARLDELGGVYLQFEEGLETTAL FVAATYKLMDHVGTEPSIKEDQVIQLMNAIFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSAS DTHEQAILRLQVTNVLSQPLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLV EVEGDNRYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFALFFQL VDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDSASGTYTLYLIIGDATL KNPILWNVADVVIKFPEEEAPSTVLSQNLFTPKQEIQHLFREPEKRPPTVVSNTFTALILSPLLLLFALW IRIGANVSNFTFAPSTIIFHLGHAAMLGLMYVYWTQLNMFQTLKYLAILGSVTFLAGNRMLAQQAVKRTA H TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002951 |
| ORF Size | 1893 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002951.5 |
| RefSeq Size | 2538 bp |
| RefSeq ORF | 1896 bp |
| Locus ID | 6185 |
| UniProt ID | P04844 |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
| MW | 69.3 kDa |
| Gene Summary | This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205852L1 | Lenti ORF clone of Human ribophorin II (RPN2), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC205852L2 | Lenti ORF clone of Human ribophorin II (RPN2), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RC205852L3 | Lenti ORF clone of Human ribophorin II (RPN2), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC205852L4 | Lenti ORF clone of Human ribophorin II (RPN2), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG205852 | RPN2 (tGFP-tagged) - Human ribophorin II (RPN2), transcript variant 1 |
CNY 5,040.00 |
|
| SC321630 | RPN2 (untagged)-Human ribophorin II (RPN2), transcript variant 1 |
CNY 4,704.00 |
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