United States
Germany
Japan
United Kingdom
China
PRPF4B (NM_003913) Human Tagged ORF Clone
CAT＃: RC205508
PRPF4B (Myc-DDK-tagged)-Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B)
ORF Plasmid: tGFP
"NM_003913" in other vectors (7)
Need custom modification / cloning service?
Get a free quote
CNY 7,016.00
CNY 7,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | dJ1013A10.1; PR4H; PRP4; PRP4H; PRP4K |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205508 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCCGCGGAGACCCAGTCGCTACGGGAGCAGCCAGAGATGGAAGATGCTAATTCTGAAAAGAGTA TAAATGAAGAAAATGGAGAAGTATCAGAAGACCAGTCTCAAAATAAGCACAGTCGTCACAAAAAAAAGAA GCATAAACACAGAAGTAAACATAAGAAACATAAACATTCCTCAGAAGAAGACAAGGATAAAAAACATAAA CATAAGCATAAACATAAGAAACACAAAAGAAAAGAGGTTATTGATGCTTCTGATAAAGAGGGTATGTCTC CAGCAAAAAGAACTAAACTTGATGATTTAGCTTTGCTAGAAGACTTGGAAAAACAGAGAGCCTTGATTAA GGCCGAACTTGATAATGAGTTAATGGAAGGAAAGGTCCAGTCTGGTATGGGGCTCATTTTGCAAGGTTAT GAGTCTGGCTCTGAAGAAGAGGGGGAAATTCATGAAAAGGCAAGAAATGGAAATAGGTCTAGTACTAGAT CTTCAAGTACAAAGGGGAAACTTGAACTTGTGGACAATAAAATTACTACAAAGAAACGAAGTAAAAGCAG ATCCAAAGAACGGACTAGACATAGGTCTGATAAAAAGAAAAGTAAGGGGGGTATTGAAATCGTTAAAGAG AAAACAACTAGGAGCAAGTCAAAGGAGAGGAAAAAATCTAAAAGCCCATCCAAAAGAAGTAAGTCTCAAG ATCAAGCAAGGAAATCAAAATCCCCTACCCTTAGAAGGCGATCTCAAGAGAAAATTGGTAAGGCCAGATC TCCTACTGATGATAAGGTTAAAATTGAAGATAAAAGTAAATCAAAAGATAGGAAAAAATCCCCAATTATA AATGAAAGTAGAAGTCGCGATCGAGGTAAAAAATCCAGATCCCCAGTTGATTTAAGAGGTAAATCCAAAG ACAGAAGGTCACGGTCCAAAGAGAGAAAATCAAAACGGTCTGAAACTGATAAAGAAAAGAAGCCAATTAA ATCTCCCTCTAAAGATGCTTCATCTGGGAAAGAAAATAGGTCACCCAGCAGAAGACCTGGTCGTAGTCCT AAAAGAAGAAGTTTGTCTCCAAAACCACGTGATAAATCAAGAAGAAGCAGGTCTCCACTTTTGAATGATA GAAGATCTAAGCAGAGCAAATCCCCCTCGCGGACACTGTCTCCTGGGAGAAGAGCCAAGAGCCGATCCTT AGAAAGAAAACGACGAGAACCAGAGAGGAGACGACTTTCTTCTCCAAGAACACGACCTCGAGATGATATC CTCAGTAGACGTGAAAGATCAAAAGATGCCAGCCCCATCAATAGATGGTCTCCAACCCGAAGAAGAAGTA GATCTCCCATTAGAAGGAGGTCTCGTTCCCCACTCAGACGTAGCAGGTCTCCAAGAAGAAGAAGCAGATC TACTCGGAGAAGGGACAGAGGTCGGAGGAGCAGATCACGCTTGCGAAGGCGGTCTCGATCACGCGGTGGT CGTAGACGAAGGAGCAGAAGCAAAGTAAAGGAAGATAAATTTAAAGGAAGTCTTTCTGAAGGAATGAAAG TTGAGCAGGAATCTTCGTCTGATGATAACCTTGAAGACTTTGATGTAGAGGAAGAAGATGAAGAAGCCCT AATAGAACAGAGAAGAATCCAAAGGCAGGCAATTGTTCAGAAATATAAATACCTTGCTGAAGATAGCAAC ATGTCTGTGCCATCTGAACCAAGCAGCCCCCAGAGCAGTACGAGAACACGATCACCATCTCCAGATGACA TTCTGGAGCGAGTAGCTGCTGATGTTAAAGAGTATGAACGGGAAAATGTTGATACATTTGAGGCCTCAGT GAAAGCCAAGCATAATCTAATGACAGTTGAACAGAATAATGGTTCATCTCAGAAGAAGTTGTTGGCACCT GATATGTTTACAGAATCTGATGATATGTTTGCTGCGTATTTTGATAGTGCTCGTCTTCGGGCCGCTGGCA TTGGAAAAGATTTCAAAGAGAATCCCAACCTCAGAGATAACTGGACCGATGCAGAAGGCTATTATCGTGT GAACATAGGTGAAGTCCTAGATAAACGTTACAATGTGTATGGCTACACTGGGCAAGGTGTATTCAGTAAT GTTGTACGAGCCAGAGATAATGCAAGAGCCAACCAAGAAGTGGCTGTAAAGATCATCAGAAACAATGAGC TCATGCAAAAGACTGGTTTAAAAGAATTAGAGTTCTTGAAAAAACTTAATGATGCTGATCCTGATGACAA ATTTCATTGTCTGAGACTCTTAAGGCACTTCTATCACAAGCAGCATCTTTGTCTGGTATTCGAGCCTCTC AGCATGAACTTACGAGAGGTGTTAAAAAAATATGGTAAAGATGTTGGTCTTCATATTAAAGCTGTAAGAT CCTATAGTCAGCAGTTGTTCCTGGCATTGAAACTCCTTAAAAGATGCAATATCCTACATGCAGATATCAA GCCAGACAATATCCTGGTTAATGAATCCAAAACTATTTTAAAGCTTTGCGATTTTGGGTCGGCTTCACAT GTTGCGGATAATGACATAACACCTTATCTTTTCAGTAGATTTTATCGTGCTCCTGAAATCATTATAGGTA AAAGCTATGACTATGGTATAGATATGTGGTCTGTAGGTTGCACCTTATACGAACTCTATACTGGAAAAAT TTTATTCCCTGGCAAAACCAATAACCATATGCTGAAGCTTGCAATGGATCTCAAAGGAAAGATGCCAAAT AAGATGATTCGAAAAGGTGTGTTCAAAGATCAGCATTTTGATCAAAATCTCAACTTCATGTACATAGAAG TTGATAAAGTAACAGAGAGGGAGAAAGTTACTGTTATGAGCACCATTAATCCAACTAAGGACCTGTTGGC TGACTTGATTGGGTGCCAGAGACTTCCTGAAGACCAACGTAAGAAAGTACACCAGCTAAAGGACTTGTTG GACCAGATTCTGATGTTGGACCCAGCTAAACGAATTAGCATCAACCAGGCCCTACAGCACGCCTTCATCC AGGAAAAAATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205508 protein sequence
Red=Cloning site Green=Tags(s) MAAAETQSLREQPEMEDANSEKSINEENGEVSEDQSQNKHSRHKKKKHKHRSKHKKHKHSSEEDKDKKHK HKHKHKKHKRKEVIDASDKEGMSPAKRTKLDDLALLEDLEKQRALIKAELDNELMEGKVQSGMGLILQGY ESGSEEEGEIHEKARNGNRSSTRSSSTKGKLELVDNKITTKKRSKSRSKERTRHRSDKKKSKGGIEIVKE KTTRSKSKERKKSKSPSKRSKSQDQARKSKSPTLRRRSQEKIGKARSPTDDKVKIEDKSKSKDRKKSPII NESRSRDRGKKSRSPVDLRGKSKDRRSRSKERKSKRSETDKEKKPIKSPSKDASSGKENRSPSRRPGRSP KRRSLSPKPRDKSRRSRSPLLNDRRSKQSKSPSRTLSPGRRAKSRSLERKRREPERRRLSSPRTRPRDDI LSRRERSKDASPINRWSPTRRRSRSPIRRRSRSPLRRSRSPRRRSRSTRRRDRGRRSRSRLRRRSRSRGG RRRRSRSKVKEDKFKGSLSEGMKVEQESSSDDNLEDFDVEEEDEEALIEQRRIQRQAIVQKYKYLAEDSN MSVPSEPSSPQSSTRTRSPSPDDILERVAADVKEYERENVDTFEASVKAKHNLMTVEQNNGSSQKKLLAP DMFTESDDMFAAYFDSARLRAAGIGKDFKENPNLRDNWTDAEGYYRVNIGEVLDKRYNVYGYTGQGVFSN VVRARDNARANQEVAVKIIRNNELMQKTGLKELEFLKKLNDADPDDKFHCLRLLRHFYHKQHLCLVFEPL SMNLREVLKKYGKDVGLHIKAVRSYSQQLFLALKLLKRCNILHADIKPDNILVNESKTILKLCDFGSASH VADNDITPYLFSRFYRAPEIIIGKSYDYGIDMWSVGCTLYELYTGKILFPGKTNNHMLKLAMDLKGKMPN KMIRKGVFKDQHFDQNLNFMYIEVDKVTEREKVTVMSTINPTKDLLADLIGCQRLPEDQRKKVHQLKDLL DQILMLDPAKRISINQALQHAFIQEKI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003913 |
| ORF Size | 3021 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003913.5 |
| RefSeq Size | 7482 bp |
| RefSeq ORF | 3024 bp |
| Locus ID | 8899 |
| UniProt ID | Q13523 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 117 kDa |
| Gene Summary | Pre-mRNA splicing occurs in two sequential transesterification steps, and the protein encoded by this gene is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs). [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205508L1 | Lenti ORF clone of Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B), Myc-DDK-tagged |
CNY 9,416.00 |
|
| RC205508L2 | Lenti ORF clone of Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B), mGFP tagged |
CNY 10,550.00 |
|
| RC205508L3 | Lenti ORF clone of Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B), Myc-DDK-tagged |
CNY 10,550.00 |
|
| RC205508L4 | Lenti ORF clone of Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B), mGFP tagged |
CNY 10,550.00 |
|
| RG205508 | PRPF4B (tGFP-tagged) - Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B) |
CNY 8,616.00 |
|
| SC107962 | PRPF4B (untagged)-Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B) |
CNY 7,016.00 |
|
| SC323624 | PRPF4B (untagged)-Kinase deficient mutant (K717M) of Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B) |
CNY 7,016.00 |
