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PIGN (NM_176787) Human Tagged ORF Clone
CAT＃: RC205334
PIGN (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 1
ORF Plasmid: tGFP
"NM_176787" in other vectors (6)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MCAHS; MCAHS1; MCD4; MDC4; PIG-N |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205334 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTGTTCTTTACTTTGGGATTGCTTATACATTTTGTGTTCTTCGCCTCCATCTTTGACATTTATT TTACATCTCCTTTGGTTCATGGAATGACTCCTCAGTTTACACCATTGCCTCCTCCAGCGAGAAGATTAGT GTTGTTTGTTGCTGATGGCCTTCGAGCAGATGCACTTTACGAATTAGATGAAAATGGAAACTCTAGAGCA CCGTTTATTAGGAATATCATAATGCATGAAGGCAGCTGGGGCATATCTCATACACGTGTGCCAACAGAAT CTCGGCCAGGTCATGTAGCTCTGATAGCTGGGTTTTATGAAGATGTCAGTGCAGTTGCCAAAGGATGGAA GGAAAATCCTGTAGAGTTTGATTCTCTTTTTAATGAAAGTAAATACACATGGAGCTGGGGAAGCCCAGAT ATCCTGCCTATGTTTGCCAAAGGTGCTAGTGGAGACCACGTTTATACATATAGTTATGATGCTAAAAGAG AGGATTTTGGTGCTCAAGATGCAACAAAACTGGATACGTGGGTTTTTGATAATGTTAAGGACTTCTTTCA TCATGCCAGAAACAACCAGTCTTTGTTTTCTAAAATAAATGAAGAGAAAATAGTTTTTTTCTTACATTTA TTAGGAATAGATACAAACGGACATGCTCATCGACCATCCTCGAGAGACTACAAGGACAATATTAAAAAAG TTGATGATGGAGTTAAAGAAATCGTGTCTATGTTTAACCATTTCTATGGAAATGATGGGAAAACAACATT TATCTTTACCTCTGACCATGGAATGACAGACTGGGGTTCCCATGGGGCTGGTCATCCTTCAGAGACTTTA ACTCCTTTAGTCACTTGGGGAGCTGGAATCAAGTATCCCCAAAGAGTATCAGCTCAGCAATTTGATGATG CATTTTTGAAAGAGTGGAGATTGGAGAATTGGAAGAGGCTAGATGTCAATCAGGCTGATATTGCACCATT GATGACTTCCCTTATTGGAGTTCCCTTTCCTCTTAACTCAGTGGGAATCCTTCCTGTGGATTATCTTAAC AACACTGATCTCTTCAAAGCAGAGAGCATGTTTACAAATGCAGTACAGATTCTTGAACAGTTCAAGGTGA AAATGACTCAGAAGAAAGAAGTTACTTTACCATTTTTGTTTACACCATTTAAACTGCTTTCTGATTCCAA ACAGTTCAACATTTTAAGAAAAGCAAGATCTTATATAAAACACAGAAAGTTTGATGAAGTGGTCTCCCTT TGCAAGGAGCTAATTCATCTTGCATTGAAAGGATTGTCCTATTATCACACATATGACAGATTCTTTTTGG GCGTCAATGTTGTTATTGGTTTTGTGGGATGGATATCTTATGCCTCTTTGTTGATCATCAAGTCTCATTC CAACCTTATAAAAGGTGTTAGTAAAGAAGTGAAGAAACCAAGCCATCTCCTGCCTTGTAGTTTTGTAGCT ATTGGCATTTTAGTAGCATTTTTTCTGCTGATTCAAGCCTGTCCCTGGACATATTATGTATATGGTTTGT TGCCACTGCCAATATGGTATGCGGTTCTAAGAGAATTTCAAGTTATTCAGGACCTTGTTGTATCAGTGTT GACCTATCCTCTGAGCCATTTTGTTGGGTACCTGTTAGCCTTTACCCTGGGAATTGAAGTATTAGTTCTC AGTTTTTTCTACCGCTATATGCTTACCGCTGGACTTACTGCCTTTGCAGCTTGGCCATTTCTCACTCGGC TGTGGACTCGAGCAAAGATGACCTCACTGAGTTGGACTTTCTTCTCTTTGCTCCTGGCAGTGTTCCCACT GATGCCGGTTGTAGGTCGAAAGCCAGACATCTCTCTAGTGATGGGTGCAGGCTTGCTGGTTCTTCTGTTA TCCCTGTGTGTTGTAACATCTCTCATGAAAAGAAAAGATAGCTTTATAAAGGAAGAGCTATTGGTACATC TGTTACAGGTGCTGAGCACAGTGCTCTCCATGTATGTTGTGTATAGCACTCAGAGTAGTCTACTCAGGAA GCAAGGACTGCCTCTCATGAATCAAATTATTAGCTGGGCAACATTAGCCTCTTCCTTGGTTGTGCCACTA CTGAGTTCTCCAGTTCTCTTTCAGCGATTGTTCAGCATACTTCTTTCATTGATGTCAACCTACCTACTTC TAAGCACAGGGTATGAAGCTCTCTTTCCACTAGTGTTGTCTTGTTTGATGTTTGTCTGGATAAACATAGA ACAAGAAACTCTACAACAATCTGGTGTTTGCTGTAAACAAAAGCTCACCAGTATCCAGTTCTCTTATAAT ACTGATATAACTCAGTTTCGACAGCTATATCTGGATGACATCCGTAGGGCCTTTTTCCTTGTTTTCTTCT TAGTGACAGCATTTTTTGGAACTGGAAATATAGCTTCTATTAACAGCTTTGATCTTGCCTCTGTCTATTG CTTTCTGACTGTGTTCAGTCCTTTTATGATGGGAGCCCTGATGATGTGGAAGATTTTAATCCCCTTTGTT CTTGTTATGTGTGCTTTTGAAGCAGTTCAGTTGACTACTCAGTTATCGTCAAAAAGCCTTTTTCTCATTG TTCTCGTCATATCAGACATTATGGCTTTGCATTTTTTCTTCTTGGTCAAGGATTATGGCAGCTGGCTTGA TATTGGGACAAGCATCAGCCACTATGTGATTGTCATGTCCATGACCATCTTTTTGGTGTTCCTCAATGGC CTGGCCCAGCTGCTCACAACGAAGAAACTCAGACTATGTGGCAAACCCAAAAGTCACTTCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205334 protein sequence
Red=Cloning site Green=Tags(s) MLLFFTLGLLIHFVFFASIFDIYFTSPLVHGMTPQFTPLPPPARRLVLFVADGLRADALYELDENGNSRA PFIRNIIMHEGSWGISHTRVPTESRPGHVALIAGFYEDVSAVAKGWKENPVEFDSLFNESKYTWSWGSPD ILPMFAKGASGDHVYTYSYDAKREDFGAQDATKLDTWVFDNVKDFFHHARNNQSLFSKINEEKIVFFLHL LGIDTNGHAHRPSSRDYKDNIKKVDDGVKEIVSMFNHFYGNDGKTTFIFTSDHGMTDWGSHGAGHPSETL TPLVTWGAGIKYPQRVSAQQFDDAFLKEWRLENWKRLDVNQADIAPLMTSLIGVPFPLNSVGILPVDYLN NTDLFKAESMFTNAVQILEQFKVKMTQKKEVTLPFLFTPFKLLSDSKQFNILRKARSYIKHRKFDEVVSL CKELIHLALKGLSYYHTYDRFFLGVNVVIGFVGWISYASLLIIKSHSNLIKGVSKEVKKPSHLLPCSFVA IGILVAFFLLIQACPWTYYVYGLLPLPIWYAVLREFQVIQDLVVSVLTYPLSHFVGYLLAFTLGIEVLVL SFFYRYMLTAGLTAFAAWPFLTRLWTRAKMTSLSWTFFSLLLAVFPLMPVVGRKPDISLVMGAGLLVLLL SLCVVTSLMKRKDSFIKEELLVHLLQVLSTVLSMYVVYSTQSSLLRKQGLPLMNQIISWATLASSLVVPL LSSPVLFQRLFSILLSLMSTYLLLSTGYEALFPLVLSCLMFVWINIEQETLQQSGVCCKQKLTSIQFSYN TDITQFRQLYLDDIRRAFFLVFFLVTAFFGTGNIASINSFDLASVYCFLTVFSPFMMGALMMWKILIPFV LVMCAFEAVQLTTQLSSKSLFLIVLVISDIMALHFFFLVKDYGSWLDIGTSISHYVIVMSMTIFLVFLNG LAQLLTTKKLRLCGKPKSHFM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_176787 |
| ORF Size | 2793 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_176787.5 |
| RefSeq Size | 4893 bp |
| RefSeq ORF | 2796 bp |
| Locus ID | 23556 |
| UniProt ID | O95427 |
| Protein Families | Transmembrane |
| Protein Pathways | Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways |
| MW | 105.8 kDa |
| Gene Summary | This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is expressed in the endoplasmic reticulum and transfers phosphoethanolamine (EtNP) to the first mannose of the GPI anchor. Two alternatively spliced variants, which encode an identical isoform, have been reported. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205334L1 | Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205334L2 | Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC205334L3 | Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205334L4 | Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG205334 | PIGN (tGFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 1 |
CNY 4,370.00 |
|
| SC310041 | PIGN (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 1 |
CNY 7,220.00 |
