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GIRK1 (KCNJ3) (NM_002239) Human Tagged ORF Clone
CAT＃: RC205322
KCNJ3 (Myc-DDK-tagged)-Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3)
ORF Plasmid: tGFP
"NM_002239" in other vectors (6)
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CNY 3,728.00
CNY 4,560.00
| Cited in 2 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GIRK1; KGA; KIR3.1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205322 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGCACTCCGAAGGAAATTTGGGGACGATTATCAGGTAGTGACCACATCGTCCAGCGGCTCGGGCT TGCAGCCCCAGGGGCCAGGCCAGGACCCTCAGCAGCAGCTTGTGCCCAAGAAGAAGCGGCAGCGGTTCGT GGACAAGAACGGCCGGTGCAATGTACAGCACGGCAACCTGGGCAGCGAGACAAGCCGCTACCTCTCGGAC CTCTTCACCACGCTGGTGGACCTCAAGTGGCGCTGGAACCTCTTCATCTTCATTCTCACCTACACCGTGG CCTGGCTTTTCATGGCGTCCATGTGGTGGGTGATCGCCTACACTCGGGGCGACCTGAACAAAGCCCACGT CGGTAACTACACGCCTTGCGTGGCCAATGTCTATAACTTCCCTTCTGCCTTCCTCTTCTTCATCGAGACG GAGGCCACCATCGGCTATGGCTACCGATACATCACAGACAAGTGCCCCGAGGGCATCATCCTCTTCCTCT TCCAGTCCATCCTGGGCTCCATCGTGGACGCCTTCCTCATCGGCTGCATGTTCATCAAGATGTCCCAGCC CAAGAAGCGCGCCGAGACCCTCATGTTCAGCGAGCACGCGGTGATCTCCATGAGGGACGGAAAACTCACG CTTATGTTCCGGGTGGGCAACCTGCGCAACAGCCACATGGTCTCCGCGCAGATTCGCTGCAAGCTGCTCA AATCTCGGCAGACACCTGAGGGTGAGTTCCTTCCCCTTGACCAACTTGAACTGGATGTAGGTTTTAGTAC AGGGGCAGATCAACTTTTTCTTGTGTCCCCCCTCACAATTCGCCACGTGATCGATGCCAAAAGCCCCTTT TATGACCTATCCCAGCGAAGCATGCAAACTAAACAGTTCGAGATTGTCGTCATCCTAGAAGGCATTGTGG AAACAACTGGGATGACTTGTCAAGCTCGAACATCATATACTGAAGATGAAGTTCTTTGGGGTCATCGTTT TTTTCCTGTAATTTCCTTAGAAGAGGGATTCTTTAAAGTTGATTACTCCCAGTTCCACGCAACATTTGAA GTCCCCACCCCACCTTACAGTGTGAAAGAGCAGGAGGAAATGCTTCTCATGTCGTCCCCTTTAATAGCAC CAGCCATAACTAACAGCAAAGAAAGACATAATTCTGTGGAATGCTTAGATGGACTAGATGATATTACTAC AAAACTACCATCTAAGCTGCAGAAAATTACTGGAAGAGAAGACTTTCCCAAAAAACTCTTGAGGATGAGT TCTACAACTTCAGAAAAAGCCTACAGCTTGGGAGACTTGCCCATGAAACTTCAACGAATAAGTTCAGTTC CGGGCAACTCAGAAGAAAAACTGGTATCTAAAACCACCAAGATGTTATCTGATCCCATGAGCCAGTCTGT GGCTGATTTGCCACCAAAGCTTCAAAAGATGGCTGGAGGAGCAGCTAGGATGGAAGGGAACCTTCCAGCC AAATTAAGAAAAATGAACTCTGATCGCTTCACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205322 protein sequence
Red=Cloning site Green=Tags(s) MSALRRKFGDDYQVVTTSSSGSGLQPQGPGQDPQQQLVPKKKRQRFVDKNGRCNVQHGNLGSETSRYLSD LFTTLVDLKWRWNLFIFILTYTVAWLFMASMWWVIAYTRGDLNKAHVGNYTPCVANVYNFPSAFLFFIET EATIGYGYRYITDKCPEGIILFLFQSILGSIVDAFLIGCMFIKMSQPKKRAETLMFSEHAVISMRDGKLT LMFRVGNLRNSHMVSAQIRCKLLKSRQTPEGEFLPLDQLELDVGFSTGADQLFLVSPLTIRHVIDAKSPF YDLSQRSMQTKQFEIVVILEGIVETTGMTCQARTSYTEDEVLWGHRFFPVISLEEGFFKVDYSQFHATFE VPTPPYSVKEQEEMLLMSSPLIAPAITNSKERHNSVECLDGLDDITTKLPSKLQKITGREDFPKKLLRMS STTSEKAYSLGDLPMKLQRISSVPGNSEEKLVSKTTKMLSDPMSQSVADLPPKLQKMAGGAARMEGNLPA KLRKMNSDRFT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002239 |
| ORF Size | 1503 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002239.4 |
| RefSeq Size | 4740 bp |
| RefSeq ORF | 1506 bp |
| Locus ID | 3760 |
| UniProt ID | P48549 |
| Domains | IRK |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| MW | 56.7 kDa |
| Gene Summary | Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and plays an important role in regulating heartbeat. It associates with three other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex that also couples to neurotransmitter receptors in the brain and whereby channel activation can inhibit action potential firing by hyperpolarizing the plasma membrane. These multimeric G-protein-gated inwardly-rectifying potassium (GIRK) channels may play a role in the pathophysiology of epilepsy, addiction, Down's syndrome, ataxia, and Parkinson's disease. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, May 2012] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
G protein signaling-biased agonism at the κ-opioid receptor is maintained in striatal neurons
,Ho, JH;Stahl, EL;Schmid, CL;Scarry, SM;Aubé, J;Bohn, LM;,
Sci Signal
,PubMed ID 30087177
[GIRK1]
|
|
Discovery and Characterization of VU0529331, a Synthetic Small-Molecule Activator of Homomeric G Protein-gated, Inwardly-rectifying, Potassium (GIRK) Channels
,Kozek, KA;Du, Y;Sharma, S;Prael, FJ;Spitznagel, BD;Kharade, SV;Denton, JS;Hopkins, CR;Weaver, CD;,
ACS Chem Neurosci
,PubMed ID 30136838
[GIRK1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205322L1 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3), Myc-DDK-tagged |
CNY 6,128.00 |
|
| RC205322L2 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3), mGFP tagged |
CNY 6,460.00 |
|
| RC205322L3 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3), Myc-DDK-tagged |
CNY 6,128.00 |
|
| RC205322L4 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3), mGFP tagged |
CNY 6,460.00 |
|
| RG205322 | KCNJ3 (tGFP-tagged) - Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3) |
CNY 5,040.00 |
|
| SC118769 | KCNJ3 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3) |
CNY 5,608.00 |
