GJA1 (NM_000165) Human Tagged ORF Clone
CAT#: RC205303
GJA1 (Myc-DDK-tagged)-Human gap junction protein, alpha 1, 43kDa (GJA1)
ORF Plasmid: tGFP
"NM_000165" in other vectors (6)
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CNY 5,488.00
Cited in 4 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AVSD3; CMDR; CX43; EKVP; EKVP3; GJAL; HLHS1; HSS; ODDD; PPKCA |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC205303 representing NM_000165
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGACTGGAGCGCCTTAGGCAAACTCCTTGACAAGGTTCAAGCCTACTCAACTGCTGGAGGGAAGG TGTGGCTGTCAGTACTTTTCATTTTCCGAATCCTGCTGCTGGGGACAGCGGTTGAGTCAGCCTGGGGAGA TGAGCAGTCTGCCTTTCGTTGTAACACTCAGCAACCTGGTTGTGAAAATGTCTGCTATGACAAGTCTTTC CCAATCTCTCATGTGCGCTTCTGGGTCCTGCAGATCATATTTGTGTCTGTACCCACACTCTTGTACCTGG CTCATGTGTTCTATGTGATGCGAAAGGAAGAGAAACTGAACAAGAAAGAGGAAGAACTCAAGGTTGCCCA AACTGATGGTGTCAATGTGGACATGCACTTGAAGCAGATTGAGATAAAGAAGTTCAAGTACGGTATTGAA GAGCATGGTAAGGTGAAAATGCGAGGGGGGTTGCTGCGAACCTACATCATCAGTATCCTCTTCAAGTCTA TCTTTGAGGTGGCCTTCTTGCTGATCCAGTGGTACATCTATGGATTCAGCTTGAGTGCTGTTTACACTTG CAAAAGAGATCCCTGCCCACATCAGGTGGACTGTTTCCTCTCTCGCCCCACGGAGAAAACCATCTTCATC ATCTTCATGCTGGTGGTGTCCTTGGTGTCCCTGGCCTTGAATATCATTGAACTCTTCTATGTTTTCTTCA AGGGCGTTAAGGATCGGGTTAAGGGAAAGAGCGACCCTTACCATGCGACCAGTGGTGCGCTGAGCCCTGC CAAAGACTGTGGGTCTCAAAAATATGCTTATTTCAATGGCTGCTCCTCACCAACCGCTCCCCTCTCGCCT ATGTCTCCTCCTGGGTACAAGCTGGTTACTGGCGACAGAAACAATTCTTCTTGCCGCAATTACAACAAGC AAGCAAGTGAGCAAAACTGGGCTAATTACAGTGCAGAACAAAATCGAATGGGGCAGGCGGGAAGCACCAT CTCTAACTCCCATGCACAGCCTTTTGATTTCCCCGATGATAACCAGAATTCAAAAAAACTAGCTGCTGGA CATGAATTACAGCCACTAGCCATTGTGGACCAGCGACCTTCAAGCAGAGCCAGCAGTCGTGCCAGCAGCA GACCTCGGCCTGATGACCTGGAGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205303 representing NM_000165
Red=Cloning site Green=Tags(s) MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAWGDEQSAFRCNTQQPGCENVCYDKSF PISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEELKVAQTDGVNVDMHLKQIEIKKFKYGIE EHGKVKMRGGLLRTYIISILFKSIFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFI IFMLVVSLVSLALNIIELFYVFFKGVKDRVKGKSDPYHATSGALSPAKDCGSQKYAYFNGCSSPTAPLSP MSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLAAG HELQPLAIVDQRPSSRASSRASSRPRPDDLEI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_000165 |
ORF Size | 1146 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000165.5 |
RefSeq Size | 3130 bp |
RefSeq ORF | 1149 bp |
Locus ID | 2697 |
UniProt ID | P17302 |
Domains | CNX, Connexin43 |
Protein Families | Druggable Genome, Ion Channels: Other, Transmembrane |
Protein Pathways | Arrhythmogenic right ventricular cardiomyopathy (ARVC), Gap junction |
MW | 42.8 kDa |
Gene Summary | This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations. [provided by RefSeq, May 2014] |
Citations (4)
The use of this cDNA Clones has been cited in the following citations: |
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4-Hydroxynonenal induces Cx46 hemichannel inhibition through its carbonylation
,Retamal, M;Fiori, M;Fernandez-Olivares, A;Linsambarth, S;Peña, F;Quintana, D;Stehberg, J;Altenberg, G;,
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
,PubMed ID 32244060
[GJA1]
|
S-nitrosylation of Connexin43 hemichannels elicits cardiac stress induced arrhythmias in Duchenne Muscular Dystrophy mice
,Lillo, MA;Himelman, E;Shirokova, N;Xie, LH;Fraidenraich, D;Contreras, JE;,
JCI Insight
,PubMed ID 31751316
[GJA1]
|
Carcinoma-astrocyte gap junctions promote brain metastasis by cGAMP transfer
,Chen, Q;Boire, A;Jin, X;Valiente, M;Er, EE;Lopez-Soto, A;Jacob, LS;Patwa, R;Shah, H;Xu, K;Cross, JR;Massagué, J;,
Nature
,PubMed ID 27225120
[GJA1]
|
Homo sapiens Systemic RNA Interference-defective-1 Transmembrane Family Member 1 (SIDT1) Protein Mediates Contact-dependent Small RNA Transfer and MicroRNA-21-driven Chemoresistance
,Mohamed O. Elhassan, Jennifer Christie, and Mark S. Duxbury,
J. Biol. Chem., Feb 2012; 287: 5267 - 5277.
[GJA1]
|
Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205303L1 | Lenti ORF clone of Human gap junction protein, alpha 1, 43kDa (GJA1), Myc-DDK-tagged |
CNY 7,888.00 |
|
RC205303L2 | Lenti ORF clone of Human gap junction protein, alpha 1, 43kDa (GJA1), mGFP tagged |
CNY 7,888.00 |
|
RC205303L3 | Lenti ORF clone of Human gap junction protein, alpha 1, 43kDa (GJA1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC205303L4 | Lenti ORF clone of Human gap junction protein, alpha 1, 43kDa (GJA1), mGFP tagged |
CNY 7,888.00 |
|
RG205303 | GJA1 (tGFP-tagged) - Human gap junction protein, alpha 1, 43kDa (GJA1) |
CNY 7,088.00 |
|
SC125548 | GJA1 (untagged)-Human gap junction protein, alpha 1, 43kDa (GJA1) |
CNY 5,488.00 |