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SRPK2 (NM_182691) Human Tagged ORF Clone
CAT＃: RC205134
SRPK2 (Myc-DDK-tagged)-Human SRSF protein kinase 2 (SRPK2), transcript variant 2
ORF Plasmid: tGFP
"NM_182691" in other vectors (8)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SFRSK2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205134 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGTTAACTCTGAGAAGTCGTCCTCTTCAGAAAGGCCGGAGCCTCAACAGAAAGCTCCTTTAGTTC CTCCTCCTCCACCGCCACCACCACCACCACCGCCACCTTTGCCAGACCCCACACCCCCGGAGCCAGAGGA GGAGATCCTGGGATCAGATGATGAGGAGCAAGAGGACCCTGCGGACTACTGCAAAGGTGGATATCATCCA GTGAAAATTGGAGACCTCTTCAATGGCCGGTATCATGTTATTAGAAAGCTTGGATGGGGGCACTTCTCTA CTGTCTGGCTGTGCTGGGATATGCAGGGGAAAAGATTTGTTGCAATGAAAGTTGTAAAAAGTGCCCAGCA TTATACGGAGACAGCCTTGGATGAAATAAAATTGCTCAAATGTGTTCGAGAAAGTGATCCCAGTGACCCA AACAAAGACATGGTGGTCCAGCTCATTGACGACTTCAAGATTTCAGGCATGAATGGGATACATGTCTGCA TGGTCTTCGAAGTACTTGGCCACCATCTCCTCAAGTGGATCATCAAATCCAACTATCAAGGCCTCCCAGT ACGTTGTGTGAAGAGTATCATTCGACAGGTCCTTCAAGGGTTAGATTACTTACACAGTAAGTGCAAGATC ATTCATACTGACATAAAGCCGGAAAATATCTTGATGTGTGTGGATGATGCATATGTGAGAAGAATGGCAG CTGAGGCCACTGAGTGGCAGAAAGCAGGTGCTCCTCCTCCTTCAGGGTCTGCAGTGAGTACGGCTCCACA GCAGAAACCTATAGGAAAAATATCTAAAAACAAAAAGAAAAAACTGAAAAAGAAACAGAAGAGGCAGGCT GAGTTATTGGAGAAGCGCCTGCAGGAGATAGAAGAATTGGAGCGAGAAGCTGAAAGGAAAATAATAGAAG AAAACATCACCTCAGCTGCACCTTCCAATGACCAGGATGGCGAATACTGCCCAGAGGTGAAACTAAAAAC AACAGGATTAGAGGAGGCGGCTGAGGCAGAGACTGCAAAGGACAATGGTGAAGCTGAGGACCAGGAAGAG AAAGAAGATGCTGAGAAAGAAAACATTGAAAAAGATGAAGATGATGTAGATCAGGAACTTGCGAACATAG ACCCTACGTGGATAGAATCACCTAAAACCAATGGCCATATTGAGAATGGCCCATTCTCACTGGAGCAGCA ACTGGACGATGAAGATGATGATGAAGAAGACTGCCCAAATCCTGAGGAATATAATCTTGATGAGCCAAAT GCAGAAAGTGATTACACATATAGCAGCTCCTATGAACAATTCAATGGTGAATTGCCAAATGGACGACATA AAATTCCCGAGTCACAGTTCCCAGAGTTTTCCACCTCGTTGTTCTCTGGATCCTTAGAACCTGTGGCCTG CGGCTCTGTGCTTTCTGAGGGATCACCACTTACTGAGCAAGAGGAGAGCAGTCCATCCCATGACAGAAGC AGAACGGTTTCAGCCTCCAGTACTGGGGATTTGCCAAAAGCAAAAACCCGGGCAGCTGACTTGTTGGTGA ATCCCCTGGATCCGCGGAATGCAGATAAAATTAGAGTAAAAATTGCTGACCTGGGAAATGCTTGTTGGGT GCATAAACACTTCACGGAAGACATCCAGACGCGTCAGTACCGCTCCATAGAGGTTTTAATAGGAGCGGGG TACAGCACCCCTGCGGACATCTGGAGCACGGCGTGTATGGCATTTGAGCTGGCAACGGGAGATTATTTGT TTGAACCACATTCTGGGGAAGACTATTCCAGAGACGAAGACCACATAGCCCACATCATAGAGCTGCTAGG CAGTATTCCAAGGCACTTTGCTCTATCTGGAAAATATTCTCGGGAATTCTTCAATCGCAGAGGAGAACTG CGACACATCACCAAGCTGAAGCCCTGGAGCCTCTTTGATGTACTTGTGGAAAAGTATGGCTGGCCCCATG AAGATGCTGCACAGTTTACAGATTTCCTGATCCCGATGTTAGAAATGGTTCCAGAAAAACGAGCCTCAGC TGGCGAATGCCTTCGGCATCCTTGGTTGAATTCT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC205134 protein sequence
Red=Cloning site Green=Tags(s) MSVNSEKSSSSERPEPQQKAPLVPPPPPPPPPPPPPLPDPTPPEPEEEILGSDDEEQEDPADYCKGGYHP VKIGDLFNGRYHVIRKLGWGHFSTVWLCWDMQGKRFVAMKVVKSAQHYTETALDEIKLLKCVRESDPSDP NKDMVVQLIDDFKISGMNGIHVCMVFEVLGHHLLKWIIKSNYQGLPVRCVKSIIRQVLQGLDYLHSKCKI IHTDIKPENILMCVDDAYVRRMAAEATEWQKAGAPPPSGSAVSTAPQQKPIGKISKNKKKKLKKKQKRQA ELLEKRLQEIEELEREAERKIIEENITSAAPSNDQDGEYCPEVKLKTTGLEEAAEAETAKDNGEAEDQEE KEDAEKENIEKDEDDVDQELANIDPTWIESPKTNGHIENGPFSLEQQLDDEDDDEEDCPNPEEYNLDEPN AESDYTYSSSYEQFNGELPNGRHKIPESQFPEFSTSLFSGSLEPVACGSVLSEGSPLTEQEESSPSHDRS RTVSASSTGDLPKAKTRAADLLVNPLDPRNADKIRVKIADLGNACWVHKHFTEDIQTRQYRSIEVLIGAG YSTPADIWSTACMAFELATGDYLFEPHSGEDYSRDEDHIAHIIELLGSIPRHFALSGKYSREFFNRRGEL RHITKLKPWSLFDVLVEKYGWPHEDAAQFTDFLIPMLEMVPEKRASAGECLRHPWLNS SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_182691 |
| ORF Size | 2064 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_182691.2 |
| RefSeq Size | 3780 bp |
| RefSeq ORF | 2067 bp |
| Locus ID | 6733 |
| UniProt ID | P78362 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 77.5 kDa |
| Gene Summary | Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation. Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28. Can mediate hepatitis B virus (HBV) core protein phosphorylation. Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205134L1 | Lenti ORF clone of Human SRSF protein kinase 2 (SRPK2), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205134L2 | Lenti ORF clone of Human SRSF protein kinase 2 (SRPK2), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC205134L3 | Lenti ORF clone of Human SRSF protein kinase 2 (SRPK2), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205134L4 | Lenti ORF clone of Human SRSF protein kinase 2 (SRPK2), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG205134 | SRPK2 (tGFP-tagged) - Human SRSF protein kinase 2 (SRPK2), transcript variant 2 |
CNY 4,370.00 |
|
| SC107542 | SRPK2 (untagged)-Human SRSF protein kinase 2 (SRPK2), transcript variant 2 |
CNY 2,950.00 |
|
| SC322514 | SRPK2 (untagged)-Human SRSF protein kinase 2 (SRPK2), transcript variant 2 |
CNY 2,950.00 |
|
| SC323336 | SRPK2 (untagged)-Kinase deficient mutant (K121M) of Human SFRS protein kinase 2 (SRPK2), transcript variant 2 |
CNY 5,048.00 |
