VAMP5 (NM_006634) Human Tagged ORF Clone
CAT#: RC204973
VAMP5 (Myc-DDK-tagged)-Human vesicle-associated membrane protein 5 (myobrevin) (VAMP5)
ORF Plasmid: tGFP
"NM_006634" in other vectors (4)
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CNY 1,200.00
CNY 3,705.00
Cited in 1 publication. |
CNY 300.00
CNY 1,999.00
CNY 3,340.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC204973 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGAATAGAGTTGGAGCGGTGCCAGCAGCAGGCGAACGAGGTGACGGAAATTATGCGTAACAACT TCGGCAAGGTCCTGGAGCGTGGTGTGAAGCTGGCCGAACTGCAGCAGCGTTCAGACCAACTCCTGGATAT GAGCTCAACCTTCAACAAGACTACACAGAACCTGGCCCAGAAGAAGTGCTGGGAGAACATCCGTTACCGG ATCTGCGTGGGGCTGGTGGTGGTTGGTGTCCTGCTCATCATCCTGATTGTGCTGCTGGTCGTCTTTCTCC CTCAGAGCAGTGACAGCAGTAGTGCCCCACGGACCCAGGATGCAGGCATTGCCTCAGGGCCTGGGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204973 protein sequence
Red=Cloning site Green=Tags(s) MAGIELERCQQQANEVTEIMRNNFGKVLERGVKLAELQQRSDQLLDMSSTFNKTTQNLAQKKCWENIRYR ICVGLVVVGVLLIILIVLLVVFLPQSSDSSSAPRTQDAGIASGPGN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_006634 |
ORF Size | 348 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_006634.3 |
RefSeq Size | 694 bp |
RefSeq ORF | 351 bp |
Locus ID | 10791 |
UniProt ID | O95183 |
Protein Families | Transmembrane |
Protein Pathways | SNARE interactions in vesicular transport |
MW | 12.8 kDa |
Gene Summary | Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of vesicles and cell membranes. The VAMP5 gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. This VAMP family member may participate in vesicle trafficking events that are associated with myogenesis. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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A reference library for assigning protein subcellular localizations by image-based machine learning
,Schormann, W;Hariharan, S;Andrews, DW;,
J. Cell Biol.
,PubMed ID 31968357
[VAMP5]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204973L3 | Lenti ORF clone of Human vesicle-associated membrane protein 5 (myobrevin) (VAMP5), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC204973L4 | Lenti ORF clone of Human vesicle-associated membrane protein 5 (myobrevin) (VAMP5), mGFP tagged |
CNY 5,890.00 |
|
RG204973 | VAMP5 (tGFP-tagged) - Human vesicle-associated membrane protein 5 (myobrevin) (VAMP5) |
CNY 2,800.00 |
|
SC122751 | VAMP5 (untagged)-Human vesicle-associated membrane protein 5 (myobrevin) (VAMP5) |
CNY 1,200.00 |