United States
Germany
Japan
United Kingdom
China
MANBA (NM_005908) Human Tagged ORF Clone
CAT＃: RC204862
MANBA (Myc-DDK-tagged)-Human mannosidase, beta A, lysosomal (MANBA)
ORF Plasmid: tGFP
"NM_005908" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 6,440.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MANB1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204862 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCCTCCACCTGCTCCTGCTGCTCGCGCTGTGCGGTGCAGGCACCACCGCCGCGGAGCTCAGTTACA GCTTGCGTGGCAACTGGAGCATCTGCAATGGGAACGGCTCGCTGGAGCTGCCCGGGGCGGTCCCTGGCTG CGTGCACAGCGCCTTGTTCCAGCAGGGCCTGATCCAGGATTCTTACTACAGATTTAATGACCTTAACTAC AGATGGGTCTCTTTGGATAACTGGACCTATAGCAAAGAATTTAAAATCCCCTTTGAAATTAGCAAATGGC AAAAAGTAAATTTGATTCTTGAGGGAGTGGATACGGTTTCAAAAATCCTGTTCAATGAAGTCACTATTGG GGAAACAGACAATATGTTCAATAGATATAGCTTTGATATTACCAACGTGGTCAGGGACGTGAACTCCATT GAGCTGCGTTTCCAGTCAGCGGTGTTGTATGCAGCACAGCAGAGCAAAGCTCACACTCGCTACCAGGTTC CCCCAGACTGCCCTCCACTTGTGCAGAAGGGTGAATGCCATGTCAACTTTGTTCGGAAGGAGCAATGTTC CTTTAGTTGGGACTGGGGGCCTTCCTTTCCTACCCAGGGAATCTGGAAAGATGTTAGAATTGAAGCCTAT AATATTTGTCACCTGAACTACTTCACATTTTCCCCAATATATGATAAGAGTGCCCAGGAGTGGAATCTGG AAATAGAGTCTACATTTGATGTTGTCAGCTCAAAGCCAGTTGGTGGTCAAGTGATCGTAGCCATCCCTAA GTTGCAAACACAACAGACATACAGCATTGAACTTCAACCTGGGAAAAGGATTGTTGAGCTATTTGTGAAC ATTAGCAAGAATATTACTGTAGAAACTTGGTGGCCTCATGGACATGGAAACCAGACTGGGTACAACATGA CTGTTCTTTTTGAACTGGATGGAGGCTTAAATATTGAAAAATCAGCTAAGGTTTATTTTAGGACAGTGGA ACTTATAGAAGAGCCTATAAAAGGGTCTCCTGGTTTGAGTTTCTATTTCAAAATTAATGGATTTCCCATA TTTCTAAAAGGCTCAAACTGGATCCCAGCAGATTCATTCCAGGACCGAGTAACCTCTGAGTTGTTACGGC TCCTTTTACAGTCTGTTGTGGATGCTAATATGAATACTCTTCGGGTTTGGGGAGGAGGAATTTATGAGCA GGATGAATTCTATGAACTCTGTGATGAACTAGGAATAATGGTATGGCAGGATTTTATGTTTGCCTGTGCC CTTTATCCAACTGATCAGGGCTTCCTGGATTCAGTGACAGCAGAAGTTGCCTACCAGATCAAGAGACTGA AATCTCATCCTTCTATCATCATATGGAGTGGCAATAATGAAAATGAGGAGGCGCTGATGATGAATTGGTA TCATATCAGTTTCACTGACCGGCCAATCTACATCAAGGACTATGTGACACTCTATGTGAAAAACATCAGA GAGCTCGTACTGGCAGGAGACAAGAGTCGTCCTTTTATTACGTCCAGTCCTACAAATGGGGCTGAAACTG TTGCAGAAGCCTGGGTCTCTCAAAACCCTAATAGCAATTATTTTGGTGATGTACATTTTTATGACTATAT CAGTGATTGCTGGAACTGGAAAGTTTTCCCAAAAGCTCGATTTGCATCTGAATATGGATATCAGTCCTGG CCGTCCTTCAGTACATTAGAAAAGGTCTCGTCTACAGAGGACTGGTCTTTCAATAGCAAGTTTTCACTTC ATCGACAACATCACGAAGGTGGTAACAAACAAATGCTTTATCAGGCTGGACTTCATTTCAAACTCCCCCA AAGCACAGATCCATTACGCACATTTAAAGATACCATCTACCTTACTCAGGTGATGCAGGCCCAGTGTGTC AAAACAGAAACTGAATTCTACCGCCGTAGTCGCAGCGAGATAGTGGATCAGCAAGGGCACACGATGGGGG CACTTTATTGGCAGTTGAATGACATCTGGCAAGCTCCTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG GAAAATGCTTCATTACTTTGCTCAGAATTTCTTTGCTCCACTGTTGCCAGTAGGCTTTGAGAATGAAAAC ATGTTCTATATCTATGGTGTGTCAGATCTTCACTCGGATTATTCGATGACACTCAGTGTGAGAGTCCATA CATGGAGCTCCCTGGAGCCCGTGTGCTCTCGTGTGACTGAACGTTTTGTGATGAAAGGAGGAGAGGCTGT CTGCCTTTATGAGGAGCCAGTGTCTGAATTGCTGAGGAGATGTGGGAATTGCACACGGGAAAGCTGTGTG GTTTCCTTTTACCTTTCAGCTGACCATGAACTCCTGAGCCCGACCAACTACCACTTCCTGTCCTCACCGA AGGAGGCCGTGGGGCTCTGCAAGGCGCAGATCACTGCCATCATCTCTCAGCAAGGTGACATATTTGTTTT TGACCTGGAGACCTCAGCTGTCGCTCCCTTTGTTTGGTTGGATGTAGGAAGCATCCCAGGGAGATTTAGT GACAATGGTTTCCTCATGACTGAGAAGACACGAACTATATTATTTTACCCTTGGGAGCCCACCAGCAAGA ATGAGTTGGAGCAATCTTTTCATGTGACCTCCTTAACAGATATTTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204862 protein sequence
Red=Cloning site Green=Tags(s) MRLHLLLLLALCGAGTTAAELSYSLRGNWSICNGNGSLELPGAVPGCVHSALFQQGLIQDSYYRFNDLNY RWVSLDNWTYSKEFKIPFEISKWQKVNLILEGVDTVSKILFNEVTIGETDNMFNRYSFDITNVVRDVNSI ELRFQSAVLYAAQQSKAHTRYQVPPDCPPLVQKGECHVNFVRKEQCSFSWDWGPSFPTQGIWKDVRIEAY NICHLNYFTFSPIYDKSAQEWNLEIESTFDVVSSKPVGGQVIVAIPKLQTQQTYSIELQPGKRIVELFVN ISKNITVETWWPHGHGNQTGYNMTVLFELDGGLNIEKSAKVYFRTVELIEEPIKGSPGLSFYFKINGFPI FLKGSNWIPADSFQDRVTSELLRLLLQSVVDANMNTLRVWGGGIYEQDEFYELCDELGIMVWQDFMFACA LYPTDQGFLDSVTAEVAYQIKRLKSHPSIIIWSGNNENEEALMMNWYHISFTDRPIYIKDYVTLYVKNIR ELVLAGDKSRPFITSSPTNGAETVAEAWVSQNPNSNYFGDVHFYDYISDCWNWKVFPKARFASEYGYQSW PSFSTLEKVSSTEDWSFNSKFSLHRQHHEGGNKQMLYQAGLHFKLPQSTDPLRTFKDTIYLTQVMQAQCV KTETEFYRRSRSEIVDQQGHTMGALYWQLNDIWQAPSWASLEYGGKWKMLHYFAQNFFAPLLPVGFENEN MFYIYGVSDLHSDYSMTLSVRVHTWSSLEPVCSRVTERFVMKGGEAVCLYEEPVSELLRRCGNCTRESCV VSFYLSADHELLSPTNYHFLSSPKEAVGLCKAQITAIISQQGDIFVFDLETSAVAPFVWLDVGSIPGRFS DNGFLMTEKTRTILFYPWEPTSKNELEQSFHVTSLTDIY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005908 |
| ORF Size | 2637 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005908.4 |
| RefSeq Size | 3311 bp |
| RefSeq ORF | 2640 bp |
| Locus ID | 4126 |
| UniProt ID | O00462 |
| Domains | Glyco_hydro_2_C |
| Protein Pathways | Lysosome, Other glycan degradation |
| MW | 100.9 kDa |
| Gene Summary | This gene encodes a member of the glycosyl hydrolase 2 family. The encoded protein localizes to the lysosome where it is the final exoglycosidase in the pathway for N-linked glycoprotein oligosaccharide catabolism. Mutations in this gene are associated with beta-mannosidosis, a lysosomal storage disease that has a wide spectrum of neurological involvement. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204862L3 | Lenti ORF clone of Human mannosidase, beta A, lysosomal (MANBA), Myc-DDK-tagged |
CNY 8,840.00 |
|
| RC204862L4 | Lenti ORF clone of Human mannosidase, beta A, lysosomal (MANBA), mGFP tagged |
CNY 9,030.00 |
|
| RG204862 | MANBA (tGFP-tagged) - Human mannosidase, beta A, lysosomal (MANBA) |
CNY 7,890.00 |
|
| SC116441 | MANBA (untagged)-Human mannosidase, beta A, lysosomal (MANBA) |
CNY 2,950.00 |
