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DHX58 (NM_024119) Human Tagged ORF Clone
CAT＃: RC204837
DHX58 (Myc-DDK-tagged)-Human DEXH (Asp-Glu-X-His) box polypeptide 58 (DHX58)
ORF Plasmid: tGFP
"NM_024119" in other vectors (6)
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CNY 7,448.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | D11LGP2; D11lgp2e; LGP2; RLR-3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204837 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTTCGGTCCTACCAATGGGAGGTGATCATGCCTGCCCTGGAGGGCAAGAATATCATCATCTGGC TGCCCACGGGTGCCGGGAAGACCCGGGCGGCTGCTTATGTGGCCAAGCGGCACCTAGAGACTGTGGATGG AGCCAAGGTGGTTGTATTGGTCAACAGGGTGCACCTGGTGACCCAGCATGGTGAAGAGTTCAGGCGCATG CTGGATGGACGCTGGACCGTGACAACCCTGAGTGGGGACATGGGACCACGTGCTGGCTTTGGCCACCTGG CCCGGTGCCATGACCTGCTCATCTGCACAGCAGAGCTTCTGCAGATGGCACTGACCAGCCCCGAGGAGGA GGAGCACGTGGAGCTCACTGTCTTCTCCCTGATCGTGGTGGATGAGTGCCACCACACGCACAAGGACACC GTCTACAACGTCATCATGAGCCAGTACCTAGAACTTAAACTCCAGAGGGCACAGCCGCTACCCCAGGTGC TGGGTCTCACAGCCTCCCCAGGCACTGGCGGGGCCTCCAAACTCGATGGGGCCATCAACCACGTCCTGCA GCTCTGTGCCAACTTGGACACGTGGTGCATCATGTCACCCCAGAACTGCTGCCCCCAGCTGCAGGAGCAC AGCCAACAGCCTTGCAAACAGTACAACCTCTGCCACAGGCGCAGCCAGGATCCGTTTGGGGACTTGCTGA AGAAGCTCATGGACCAAATCCATGACCACCTGGAGATGCCTGAGTTGAGCCGGAAATTTGGGACGCAAAT GTATGAGCAGCAGGTGGTGAAGCTGAGTGAGGCTGCGGCTTTGGCTGGGCTTCAGGAGCAACGGGTGTAT GCGCTTCACCTGAGGCGCTACAATGACGCGCTGCTCATCCATGACACCGTCCGCGCCGTGGATGCCTTGG CTGCGCTGCAGGATTTCTATCACAGGGAGCACGTCACTAAAACCCAGATCCTGTGTGCCGAGCGCCGGCT GCTGGCCCTGTTCGATGACCGCAAGAATGAGCTGGCCCACTTGGCAACTCATGGCCCAGAGAATCCAAAA CTGGAGATGCTGGAAAAGATCCTGCAAAGGCAGTTCAGTAGCTCTAACAGCCCTCGGGGTATCATCTTCA CCCGCACCCGCCAAAGCGCACACTCCCTCCTGCTCTGGCTCCAGCAGCAGCAGGGCCTGCAGACTGTGGA CATCCGGGCCCAGCTACTGATTGGGGCTGGGAACAGCAGCCAGAGCACCCACATGACCCAGAGGGACCAG CAAGAAGTGATCCAGAAGTTCCAAGATGGAACCCTGAACCTTCTGGTGGCCACGAGTGTGGCGGAGGAGG GGCTGGACATCCCACATTGCAATGTGGTGGTGCGTTATGGGCTCTTGACCAATGAAATCTCCATGGTCCA GGCCAGGGGCCGTGCCCGGGCCGATCAGAGTGTATACGCGTTTGTAGCAACTGAAGGTAGCCGGGAGCTG AAGCGGGAGCTGATCAACGAGGCGCTGGAGACGCTGATGGAGCAGGCAGTGGCTGCTGTGCAGAAAATGG ACCAGGCCGAGTACCAGGCCAAGATCCGGGATCTGCAGCAGGCAGCCTTGACCAAGCGGGCGGCCCAGGC AGCCCAGCGGGAGAACCAGCGGCAGCAGTTCCCAGTGGAGCACGTGCAGCTACTCTGCATCAACTGCATG GTGGCTGTGGGCCATGGCAGCGACCTGCGGAAGGTGGAGGGCACCCACCATGTCAATGTGAACCCCAACT TCTCGAACTACTATAATGTCTCCAGGGATCCTGTGGTCATCAACAAAGTCTTCAAGGACTGGAAGCCTGG GGGTGTCATCAGCTGCAGGAACTGTGGGGAGGTCTGGGGTCTGCAGATGATCTACAAGTCAGTGAAGCTG CCAGTGCTCAAAGTCCGCAGCATGCTGCTGGAGACCCCTCAGGGGCGGATCCAGGCCAAAAAGTGGTCCC GCGTGCCCTTCTCCGTGCCTGACTTTGACTTCCTGCAGCATTGTGCCGAGAACTTGTCGGACCTCTCCCT GGAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC204837 protein sequence
Red=Cloning site Green=Tags(s) MELRSYQWEVIMPALEGKNIIIWLPTGAGKTRAAAYVAKRHLETVDGAKVVVLVNRVHLVTQHGEEFRRM LDGRWTVTTLSGDMGPRAGFGHLARCHDLLICTAELLQMALTSPEEEEHVELTVFSLIVVDECHHTHKDT VYNVIMSQYLELKLQRAQPLPQVLGLTASPGTGGASKLDGAINHVLQLCANLDTWCIMSPQNCCPQLQEH SQQPCKQYNLCHRRSQDPFGDLLKKLMDQIHDHLEMPELSRKFGTQMYEQQVVKLSEAAALAGLQEQRVY ALHLRRYNDALLIHDTVRAVDALAALQDFYHREHVTKTQILCAERRLLALFDDRKNELAHLATHGPENPK LEMLEKILQRQFSSSNSPRGIIFTRTRQSAHSLLLWLQQQQGLQTVDIRAQLLIGAGNSSQSTHMTQRDQ QEVIQKFQDGTLNLLVATSVAEEGLDIPHCNVVVRYGLLTNEISMVQARGRARADQSVYAFVATEGSREL KRELINEALETLMEQAVAAVQKMDQAEYQAKIRDLQQAALTKRAAQAAQRENQRQQFPVEHVQLLCINCM VAVGHGSDLRKVEGTHHVNVNPNFSNYYNVSRDPVVINKVFKDWKPGGVISCRNCGEVWGLQMIYKSVKL PVLKVRSMLLETPQGRIQAKKWSRVPFSVPDFDFLQHCAENLSDLSLD SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024119 |
| ORF Size | 2034 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024119.3 |
| RefSeq Size | 2631 bp |
| RefSeq ORF | 2037 bp |
| Locus ID | 79132 |
| UniProt ID | Q96C10 |
| Protein Pathways | RIG-I-like receptor signaling pathway |
| MW | 76.6 kDa |
| Gene Summary | Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1-dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Quantitative Micro-Spectroscopic Imaging Reveals Viral and Cellular RNA Helicase Interactions in Live Cells
,Corby, MJ;Stoneman, MR;Biener, G;Paprocki, JD;Kolli, R;Raicu, V;Frick, DN;,
J. Biol. Chem.
,PubMed ID 28483922
[DHX58]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204837L1 | Lenti ORF clone of Human DEXH (Asp-Glu-X-His) box polypeptide 58 (DHX58), Myc-DDK-tagged |
CNY 9,848.00 |
|
| RC204837L2 | Lenti ORF clone of Human DEXH (Asp-Glu-X-His) box polypeptide 58 (DHX58), mGFP tagged |
CNY 7,700.00 |
|
| RC204837L3 | Lenti ORF clone of Human DEXH (Asp-Glu-X-His) box polypeptide 58 (DHX58), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC204837L4 | Lenti ORF clone of Human DEXH (Asp-Glu-X-His) box polypeptide 58 (DHX58), mGFP tagged |
CNY 9,848.00 |
|
| RG204837 | DHX58 (tGFP-tagged) - Human DEXH (Asp-Glu-X-His) box polypeptide 58 (DHX58) |
CNY 9,048.00 |
|
| SC122954 | DHX58 (untagged)-Human DEXH (Asp-Glu-X-His) box polypeptide 58 (DHX58) |
CNY 7,456.00 |
