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CH25H (NM_003956) Human Tagged ORF Clone
CAT＃: RC204716
CH25H (Myc-DDK-tagged)-Human cholesterol 25-hydroxylase (CH25H)
ORF Plasmid: tGFP
"NM_003956" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C25H |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204716 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTGCCACAACTGCTCCGACCCCCAGGTCCTTTGCAGCTCCGGGCAGCTGTTCCTGCAGCCCCTCT GGGACCACCTGAGGAGCTGGGAGGCCCTCCTACAGTCGCCCTTCTTCCCGGTCATCTTCTCCATCACCAC ATACGTGGGCTTTTGCCTGCCCTTCGTGGTCCTGGATATCCTGTGCTCCTGGGTGCCCGCCCTGCGGCGC TACAAGATCCACCCTGACTTCTCGCCATCCGCGCAGCAGCTGCTACCTTGCCTGGGGCAGACCCTCTACC AGCATGTGATGTTTGTGTTCCCCGTGACGCTGCTGCATTGGGCCCGCAGCCCGGCCCTCCTGCCCCACGA AGCTCCCGAGCTGCTCCTGCTGCTGCACCACATCCTGTTCTGCCTGCTACTCTTCGACATGGAGTTCTTC GTGTGGCACCTGCTGCACCACAAGGTGCCCTGGCTGTACCGCACCTTCCACAAGGTGCACCACCAGAACT CGTCCTCGTTCGCGCTGGCAACGCAGTATATGAGCGTCTGGGAACTGTTTTCTTTGGGCTTCTTCGACAT GATGAACGTCACACTGCTCGGGTGCCACCCGCTCACCACCCTGACCTTCCACGTGGTCAACATCTGGCTT TCCGTGGAGGACCACTCCGGCTACAACTTCCCTTGGTCCACTCACAGACTGGTGCCCTTCGGGTGGTACG GGGGTGTGGTGCACCACGACCTGCATCACTCTCACTTTAACTGCAACTTCGCTCCGTACTTTACACACTG GGACAAAATACTGGGAACGCTGCGGACTGCATCTGTCCCAGCGCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204716 protein sequence
Red=Cloning site Green=Tags(s) MSCHNCSDPQVLCSSGQLFLQPLWDHLRSWEALLQSPFFPVIFSITTYVGFCLPFVVLDILCSWVPALRR YKIHPDFSPSAQQLLPCLGQTLYQHVMFVFPVTLLHWARSPALLPHEAPELLLLLHHILFCLLLFDMEFF VWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELFSLGFFDMMNVTLLGCHPLTTLTFHVVNIWL SVEDHSGYNFPWSTHRLVPFGWYGGVVHHDLHHSHFNCNFAPYFTHWDKILGTLRTASVPAR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003956 |
| ORF Size | 816 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003956.4 |
| RefSeq Size | 1378 bp |
| RefSeq ORF | 819 bp |
| Locus ID | 9023 |
| UniProt ID | O95992 |
| Protein Families | Transmembrane |
| Protein Pathways | Primary bile acid biosynthesis |
| MW | 31.7 kDa |
| Gene Summary | This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
25-hydroxycholesterol Production by Interferon Stimulated Gene Cholesterol-25-hydroxylase Restricts Mammalian Reovirus Infection
,Doms, A;Sanabria, T;Hansen, JN;Altan-Bonnet, N;Holm, GH;,
J. Virol.
,PubMed ID 29950420
[CH25H]
|
|
25-Hydroxycholesterol Inhibition of Lassa Virus Infection through Aberrant GP1 Glycosylation
,Shrivastava-Ranjan, P;Bergeron, É;Chakrabarti, AK;Albariño, CG;Flint, M;Nichol, ST;Spiropoulou, CF;,
MBio
,PubMed ID 27999160
[CH25H]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204716L1 | Lenti ORF clone of Human cholesterol 25-hydroxylase (CH25H), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC204716L2 | Lenti ORF clone of Human cholesterol 25-hydroxylase (CH25H), mGFP tagged |
CNY 5,890.00 |
|
| RC204716L3 | Lenti ORF clone of Human cholesterol 25-hydroxylase (CH25H), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204716L4 | Lenti ORF clone of Human cholesterol 25-hydroxylase (CH25H), mGFP tagged |
CNY 4,800.00 |
|
| RG204716 | CH25H (tGFP-tagged) - Human cholesterol 25-hydroxylase (CH25H) |
CNY 4,000.00 |
|
| SC125616 | CH25H (untagged)-Human cholesterol 25-hydroxylase (CH25H) |
CNY 2,400.00 |
