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CNOT2 (NM_014515) Human Tagged ORF Clone
CAT＃: RC204121
CNOT2 (Myc-DDK-tagged)-Human CCR4-NOT transcription complex, subunit 2 (CNOT2), transcript variant 2
ORF Plasmid: tGFP
"NM_014515" in other vectors (4)
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CNY 6,032.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CDC36; HSPC131; IDNADFS; NOT2; NOT2H |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204121 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAGGACTGATGGACATACATTATCTGAGAAAAGAAACTACCAGGTGACAAACAGCATGTTTGGTG CTTCAAGAAAGAAGTTTGTAGAGGGGGTCGACAGTGACTACCATGACGAAAACATGTACTACAGCCAGTC TTCTATGTTTCCACATCGGTCAGAAAAAGATATGCTGGCATCACCATCTACATCAGGTCAGCTGTCTCAG TTTGGGGCAAGTTTATACGGGCAACAAAGTGCACTAGGCCTTCCAATGAGGGGGATGAGCAACAATACCC CTCAGTTAAATCGCAGCTTATCACAAGGCACTCAGTTACCGAGCCACGTCACGCCAACAACAGGGGTACC AACAATGTCACTTCACACGCCTCCATCTCCAAGCAGGGGTATTTTGCCTATGAATCCTAGGAATATGATG AACCACTCCCAGGTTGGTCAGGGCATTGGAATTCCTAGCAGGACAAATAGCATGAGCAGTTCAGGGTTAG GTAGCCCCAACAGAAGCTCGCCAAGCATAATATGTATGCCAAAGCAGCAGCCTTCTCGACAGCCTTTTAC TGTGAACAGTATGTCTGGATTTGGAATGAACAGGAATCAGGCATTTGGAATGAATAACTCCTTATCAAGT AACATTTTTAATGGAACAGACGGAAGTGAAAATGTGACAGGATTGGACCTTTCAGATTTCCCAGCATTAG CAGACCGAAACAGGAGGGAAGGAAGTGGTAACCCAACTCCATTAATAAACCCCTTGGCTGGAAGAGCTCC TTATGTTGGAATGGTAACAAAACCAGCAAATGAACAATCCCAGGACTTCTCAATACACAATGAAGATTTT CCAGCATTACCAGGCTCCAGCTATAAAGATCCAACATCAAGTAATGATGACAGTAAATCTAATTTGAATA CATCTGGCAAGACAACTTCAAGTACAGATGGACCCAAATTCCCTGGAGATAAAAGTTCAACAACACAAAA TAATAACCAGCAGAAAAAAGGGATCCAGGTGTTACCTGATGGTCGGGTTACTAACATTCCTCAAGGGATG GTGACGGACCAATTTGGAATGATTGGCCTGTTAACATTTATCAGGGCAGCAGAGACAGACCCAGGAATGG TACATCTTGCATTAGGAAGTGACTTAACAACATTAGGCCTCAATCTGAACTCTCCTGAAAATCTCTACCC CAAATTTGCGTCACCCTGGGCATCTTCACCTTGTCGACCTCAAGACATAGACTTCCATGTTCCATCTGAG TACTTAACGAACATTCACATTAGGGATAAGCTGGCTGCAATAAAACTTGGCCGATATGGTGAAGACCTTC TCTTCTATCTCTATTACATGAATGGAGGAGACGTATTACAACTTTTAGCTGCAGTGGAGCTTTTTAACCG TGATTGGAGATACCACAAAGAAGAACGAGTATGGATTACCAGGGCACCAGGCATGGAGCCAACAATGAAA ACCAATACCTATGAGAGGGGAACATATTACTTCTTTGACTGTCTTAACTGGAGGAAAGTAGCTAAGGAGT TCCATCTGGAATATGACAAATTAGAAGAACGGCCTCACCTGCCATCCACCTTCAACTACAACCCTGCTCA GCAAGCCTTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204121 protein sequence
Red=Cloning site Green=Tags(s) MVRTDGHTLSEKRNYQVTNSMFGASRKKFVEGVDSDYHDENMYYSQSSMFPHRSEKDMLASPSTSGQLSQ FGASLYGQQSALGLPMRGMSNNTPQLNRSLSQGTQLPSHVTPTTGVPTMSLHTPPSPSRGILPMNPRNMM NHSQVGQGIGIPSRTNSMSSSGLGSPNRSSPSIICMPKQQPSRQPFTVNSMSGFGMNRNQAFGMNNSLSS NIFNGTDGSENVTGLDLSDFPALADRNRREGSGNPTPLINPLAGRAPYVGMVTKPANEQSQDFSIHNEDF PALPGSSYKDPTSSNDDSKSNLNTSGKTTSSTDGPKFPGDKSSTTQNNNQQKKGIQVLPDGRVTNIPQGM VTDQFGMIGLLTFIRAAETDPGMVHLALGSDLTTLGLNLNSPENLYPKFASPWASSPCRPQDIDFHVPSE YLTNIHIRDKLAAIKLGRYGEDLLFYLYYMNGGDVLQLLAAVELFNRDWRYHKEERVWITRAPGMEPTMK TNTYERGTYYFFDCLNWRKVAKEFHLEYDKLEERPHLPSTFNYNPAQQAF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014515 |
| ORF Size | 1621 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014515.7 |
| RefSeq Size | 3293 bp |
| RefSeq ORF | 1623 bp |
| Locus ID | 4848 |
| UniProt ID | Q9NZN8 |
| Domains | NOT |
| Protein Families | Transcription Factors |
| Protein Pathways | RNA degradation |
| MW | 59.7 kDa |
| Gene Summary | This gene encodes a subunit of the multi-component CCR4-NOT complex. The CCR4-NOT complex regulates mRNA synthesis and degradation and is also thought to be involved in mRNA splicing, transport and localization. The encoded protein interacts with histone deacetylases and functions as a repressor of polymerase II transcription. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204121L3 | Lenti ORF clone of Human CCR4-NOT transcription complex, subunit 2 (CNOT2), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204121L4 | Lenti ORF clone of Human CCR4-NOT transcription complex, subunit 2 (CNOT2), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG204121 | CNOT2 (tGFP-tagged) - Human CCR4-NOT transcription complex, subunit 2 (CNOT2), transcript variant 2 |
CNY 7,632.00 |
|
| SC321450 | CNOT2 (untagged)-Human CCR4-NOT transcription complex, subunit 2 (CNOT2), transcript variant 2 |
CNY 6,040.00 |
